EBV Genomics and Bioinformatics

EBV 基因组学和生物信息学

• 批准号: 10627696
• 负责人: Italo Tempera
• 金额: $ 21.25万
• 依托单位: WISTAR INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-11 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627696
• 关键词: ATAC-seq; Bioinformatics; Carcinoma; Cell Culture Techniques; ChIP-seq; Chromatin Conformation Capture and Sequencing; Clustered Regularly Interspaced Short Palindromic Repeats; DNA Methylation; Data; Data Analyses; Data Security; Deposition; Development; Ensure; Environment; Epigenetic Process; Funding; Future; Generations; Genome; Genomic approach; Genomics; Goals; Human Herpesvirus 4; Hybrids; Instruction; Libraries; Malignant Neoplasms; Manuscripts; Metabolic Control; Methodology; Methods; Modeling; Mus; Oncology; Organoids; Preparation; Procedures; Process; Protocols documentation; Quality Control; RNA; Reagent; Reporting; Research; Research Personnel; Research Project Grants; Role; Sampling; Services; Technology; Testing; Viral; Visualization; Work; Writing; Xenograft procedure; bisulfite sequencing; data exchange; data harmonization; data management; epigenome; experimental study; functional genomics; genome analysis; insight; mRNA Expression; member; next generation sequencing; programs; public database; repository; screening; small molecule; transcriptome sequencing; tumor

CORE D – PROJECT SUMMARY The goal of the EBV Genomics and Bioinformatics Core D will be to provide unified, EBV-centric functional genomic methodologies and integrative bioinformatics support to all research Projects within the Program. Core D proposes two Specific Aims: 1) develop and implement functional genomic approaches to study viral and host genomes and epigenomes using diverse Next Generation Sequencing (NGS) approaches; and 2) provide bioinformatic support for each Project through a centralize hub responsible for data management, quality control, analysis, and transfer within the program. The scope of service will include: 1) providing expertise and capabilities in genomic approaches including mRNA expression by RNA-seq, CHIP- and ATAC-seq library preparations, DNA methylation studies by RBBS, and HiC ; 2) ensuring seamless coordination of ‘omics’ efforts across projects as they develop new insights about EBV and performing integrative analyses to combine data from different experiments across the various projects; and 3) assisting with final reports, abstracts, manuscripts, and future funding proposals. Core D’s broad, long-term objective is: to provide leading-edge functional genomics and comprehensive bioinformatics and data management support for the Program members, and proper deposit in public database repositories.

核心D -项目总结 EBV基因组学和生物信息学核心D的目标是提供统一的、以EBV为中心的功能性 基因组学方法和综合生物信息学支持计划内的所有研究项目。核心 D提出了两个具体目标：1）开发和实施功能基因组方法来研究病毒和 宿主基因组和表观基因组，使用不同的下一代测序（NGS）方法;和2）提供 通过一个负责数据管理、质量控制 分析，并在程序中传输。服务范围将包括：1）提供专业知识和能力 在包括通过RNA-seq、CHIP-和ATAC-seq文库制备的mRNA表达的基因组方法中， RBBS和HiC的DNA甲基化研究; 2）确保项目间“组学”工作的无缝协调 因为他们开发了关于EBV的新见解，并进行综合分析，将来自不同组织的联合收割机数据结合起来， 在不同的项目实验;和3）协助最终报告，摘要，手稿，和未来 供资建议。核心D的广泛，长期的目标是：提供领先的功能基因组学和 为计划成员提供全面的生物信息学和数据管理支持，并适当存款 公共数据库仓库。