Feasibility of novel Fluorine Non-thermal plasma for dental caries control

新型氟非热等离子体控制龋齿的可行性

• 批准号: 10739640
• 负责人: Liang Hong
• 金额: $ 23.78万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10739640
• 关键词: Acids; Adult; Animal Model; Area; Argon; Atmosphere; Caries prevention; Cells; Childhood; Chronic Disease; Communicable Diseases; Custom; Data; Dental; Dental Enamel; Dental caries; Dentistry; Development; Devices; Disease; Early treatment; Environment; Epithelial Cells; Exhibits; Female; Fluorides; Fluorine; Foundations; Free Radicals; Funding; Future; Gases; Generations; Gingiva; Goals; Grant; Growth; Health; Human; Hydroxyapatites; In Vitro; Interdisciplinary Study; Ions; Knowledge; Literature; Metals; Microbial Biofilms; Modeling; Odontogenesis; Oral; Oxidative Stress; Penetration; Persons; Physiological; Plasma; Predisposition; Prevention approach; Process; Production; Protocols documentation; Public Health; Publishing; Rattus; Recovery; Reporting; Research; Research Project Grants; Selection Criteria; Solid; Sprague-Dawley Rats; Streptococcus mutans; Streptococcus sanguis; Structure; Technology; Temperature; Testing; Tooth Remineralization; Tooth structure; Water fluoridation; Work; aged; biomaterial compatibility; burden of illness; cariogenic bacteria; cytotoxicity; deciduous tooth; demineralization; dental biofilm; design; experimental study; hazard; human disease; human old age (65+); in vitro Model; in vivo; innovation; novel; oral biofilm; permanent tooth; prevent; prototype; remineralization; tooth surface

Dental caries represents a multifactorial disease that results from interactions among dental biofilms, the oral environment, and tooth structure. Mounting reports in the literature demonstrated that fluoride could decrease enamel demineralization and enhance enamel remineralization, and at sufficient concentrations, could inhibit acid production by cariogenic bacteria. Fluoride is the cornerstone for dental caries prevention and remains its first line of defense. Yet despite the extensive use of fluoride in dentistry for decades, dental caries remains a serious public health problem worldwide. Limitations of current fluoride delivery technologies include low penetration of delivered fluoride into sub-surface areas and short-term retention of delivered fluoride on teeth, which prevent the full utilization of fluoride. However, few advancements in F technologies have been made in past decades to overcome these limitations. We were inspired by reports that fluorine nonthermal plasmas (FNTPs) generated from F-containing feed gases could produce free F atoms (F- ions and F free radicals) and that FNTPs have been used to synthesize various metal fluorides. Our pioneering work in this area has demonstrated that FNTPs can deliver fluoride into tooth enamel and enhance its remineralization using an in vitro pH cycling experiment. Moreover, our previous in vitro work demonstrated that both argon (Ar) and Ar/O2 nonthermal plasmas (NTPs) efficiently decrease S. mutans cells and S. mutans biofilm formation. Our in vivo study in female Sprague–Dawley rats showed that rat molars treated with 2-minute Ar/O2 plasmas exhibited less decayed tooth surfaces 6 months after the plasma treatment, 62.5% less on the upper molars and 31.6% less on the lower molars relative to untreated molars. In this application, we propose to generate biocompatible FNTPs and investigate their efficiencies on tooth enamel remineralization, fluoride delivery into enamel, and inhibition of dental biofilm recovery with the ultimate goal of developing FNTPs for control of dental caries. Our central hypothesis is that biocompatible FNTPs generated from a plasma feed gas comprised of argon (Ar) gas and a nontoxic and nonirritating F-containing gaseous compound will have the dual effects of enhancing remineralization via effective fluoridation and inhibiting biofilm recovery. Two specific aims are designed to test this hypothesis. Specific Aim 1, to generate biocompatible FNTPs with desirable fluoride delivery capabilities, and Specific Aim 2, to assess the effect and efficiency of FNTP treatment on enhanced remineralization of enamel, fluoride delivery into enamel, and inhibition of dental biofilm recovery in vitro. This proposed research project will not only contribute to our fundamental knowledge of FNTP generation and their interaction with tooth enamel and dental biofilms, but also facilitate the development of FNTPs as an innovative approach for prevention and early treatment of dental caries, thus producing a major impact in controlling this prominent hazard to human health.

牙科车是一种多因素疾病，该疾病是由牙科生物膜之间相互作用（口服 环境和牙齿结构。文献中的安装报告表明氟化物可能会减少 搪瓷脱矿化并增强牙釉质提醒，并以足够的浓度抑制 致癌细菌产生酸。氟化物是预防牙齿的基石，仍然是 第一道防线。然而，尽管数十年来广泛使用了氟化物，但牙科车仍然是 全球严重的公共卫生问题。当前氟化物输送技术的局限性包括低 将液化剂渗透到地下区域，并在牙齿上递送的液化物的短期保留率， 可以防止氟化物的全面利用。但是，F技术的进步很少 过去几十年来克服这些局限性。 我们的灵感来自报道说，含含F的Feed产生的氟非热等离子体（FNTP） 气体可以产生自由F原子（F-离子和F自由基），并且FNTP已用于合成 各种金属氟化物。我们在这一领域的开创性工作表明，FNTP可以将氟化物输送到 牙釉质并使用体外pH循环实验增强其先生化。而且，我们以前的 体外工作表明，氩（AR）和AR/O2非热等离子体（NTPS）都有效地减少了S. Mutans细胞和S. mutans生物膜形成。我们在女性Sprague -Dawley大鼠中的体内研究表明 血浆后6个月，用2分钟的AR/O2等离子体处理的磨牙暴露于衰减的牙齿表面较少 相对于未经处理的磨牙，在上层磨牙上的治疗量减少了62.5％，下层磨牙的31.6％降低了33.6％。 在此应用中，我们建议生成生物相容性的FNTP，并研究其效率 牙釉质提醒，氟化物递送到搪瓷以及抑制牙齿生物膜回收的最终 开发用于控制牙齿携带的FNTP的目标。我们的中心假设是生物相容性的FNTP 由血浆进料气体完成的氩气（AR）气体和无毒的F含F含F的血浆进料气体产生 气态化合物将通过有效氟化和 抑制生物膜恢复。两个具体的目标旨在检验这一假设。特定目标1，生成 具有理想氟化物递送功能的生物相容性FNTP和特定目标2，以评估 FNTP处理的效果和效率对增强搪瓷，氟化物递送的提醒 搪瓷，并抑制体外牙齿生物膜恢复。这个拟议的研究项目不仅将 有助于我们对FNTP生成的基本知识以及它们与牙齿搪瓷和牙齿的互动 生物膜，但也支持FNTP作为预防和早期的创新方法的发展 治疗龋齿，从而在控制这种对人类健康的巨大危害方面产生了重大影响。