ShEEP Request for a Multimodal Plate Reader

ShEEP 请求多模式读板仪

• 批准号: 10738976
• 负责人: CHRISTOPHER P CARDOZO
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10738976
• 关键词: Acceleration; Aging; Antibodies; Biochemical Reaction; Biological; Biological Assay; Biomedical Research; Cancer Biology; Chemicals; DNA; Dementia; Devices; Diagnosis; Disease; Enzyme-Linked Immunosorbent Assay; Equipment; Fluorescence; Fluorescence Polarization; Funding; Growth Factor; Health; Hormones; Kidney Diseases; Kinetics; Laboratories; Life; Light; Luciferases; Measurement; Measures; Molecular; Persian Gulf Syndrome; Proteins; Quality of life; RNA; Reader; Research; Research Design; Scanning; Services; Sheep; Specific qualifier value; Spectrophotometry; Spinal cord injury; Temperature; Testing; Time; Traumatic Brain Injury; Veterans; anticancer research; blood pressure control; bone loss; detector; improved; instrument; instrumentation; laptop; light emission; luminescence; molecular dynamics; multimodality; protein protein interaction

The requested instrument is a Molecular Dynamics Spectramax id5 multi-mode Microplate reader with dual injectors. It is used to perform assays based on spectrophotometry, fluorimetry, time- resolved fluorescence and luminescence. This type of instrument is widely used in biomedical research for measuring amounts of certain chemicals such as ATP and of biological substances such as hormones, antibodies and growth factors. It can also be used to test for specific types of protein- protein interactions. It will replace existing though aging equipment that is reaching the end of its service life. It will also be adding capabilities our current instrumentation lacks, such as dual injectors for use in flash assays such as measurement of ATP levels, and the capacity to make measurements using 384-well plates. Its availability will enhance ongoing VA-funded projects focused on reducing bone loss due to spinal cord injury, advancing understanding of the diagnosis and treatment of dementia, greater understanding of Gulf War syndrome and other disorders related to miliary service, traumatic brain injury, renal disease and blood pressure control. By doing so, the equipment will accelerate the pace of VA research aimed at improving health and quality of life of Veterans.

所需仪器为Molecular Dynamics Spectramax id 5多模式酶标仪 双喷射器。它用于进行基于分光光度法、荧光法、时间- 分辨荧光和发光。这类仪器广泛应用于生物医学领域 测量某些化学物质（如ATP）和生物物质（如 激素抗体和生长因子它也可以用来测试特定类型的蛋白质- 蛋白质相互作用它将取代现有的，虽然老化的设备，这是达到其年底， 使用寿命它还将增加我们目前的仪器所缺乏的功能，例如双喷射器 用于快速测定，例如测量ATP水平，以及进行测量的能力 使用384孔板。它的提供将加强正在进行的VA资助的项目，重点是减少 脊髓损伤引起的骨丢失，提高对脊髓损伤的诊断和治疗的认识， 痴呆症，对海湾战争综合症和其他与粟粒性疾病有关的疾病的更多了解， 创伤性脑损伤、肾病和血压控制。通过这样做，设备将 加快旨在改善退伍军人健康和生活质量的退伍军人事务部研究的步伐。