ShEEP Request for A Metabolic and Behavioral Phenotyping System

ShEEP 请求建立代谢和行为表型系统

• 批准号: 9795306
• 负责人: CHRISTOPHER P CARDOZO
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795306
• 关键词: Acoustics; Affect; Aging; Alzheimer' s Disease; Animal Behavior; Animal Experimentation; Animal Testing; Animals; Awareness; Behavior; Behavioral; Biomedical Research; Blood Pressure Monitors; Body Weight; Cognition; Color; Computer software; Computers; Core Facility; Data; Diabetes Mellitus; Disease; Engineering; Environment; Equipment; Equipment Malfunction; Evaluation; Funding; Genetic; Health; Home environment; Indirect Calorimetry; Investigation; Knowledge; Laboratories; Learning; Link; Lipids; Martes zibellina; Measurement; Measures; Medical; Medical center; Memory; Metabolic; Metabolic syndrome; Metabolism; Mind; Modality; Modernization; Mus; Neurodegenerative Disorders; Neurologic; Operative Surgical Procedures; Pharmaceutical Preparations; Phenotype; Physical Function; Physiological; Population; Quality of life; Radial; Rattus; Request for Applications; Research; Research Personnel; Research Project Grants; Research Support; Resolution; Rodent; Running; Scientist; Sheep; Shipping; Signal Transduction; Socialization; Spinal cord injury; Stress; System; Testing; Therapeutic Intervention; Training; Trauma; Traumatic Brain Injury; United States National Institutes of Health; Update; Veterans; Work; aging population; arm; behavior test; conditioned fear; diabetes risk; disability; efficacy testing; functional decline; instrument; interest; man; metabolic phenotype; mild traumatic brain injury; morris water maze; prepulse inhibition; programs; repaired; trait

Background: The equipment requested in this application will provide the capability to comprehensively evaluate metabolic and behavioral phenotypes in rats and mice. Our investigators presently use behavioral testing as an endpoint in ongoing, VA-funded research on spinal cord injury (SCI), Alzheimer's disease and mild traumatic brain injury (mTBI). Behavioral testing is also an important endpoint in ongoing NIH funded studies on aging related vulnerability to functional decline after surgery. Because of links between mTBI, Alzheimer's disease or SCI and diabetes and metabolic syndrome, there is an intense interest in having the ability to evaluate metabolic function among many of our investigators. Equipment Requested: This application requests funds to purchase additional equipment needed to update and expand our current rodent phenotyping core: 1) Fear conditioning; 2) Acoustic startle/prepulse inhibition; 3) Open field; 4) Morris water maze; 5) Escape hole radial 8-arm maze; 6) Sociability; 7) Rotorods; 8) Blood pressure monitoring; 9) Ethovision XT13 tracking software and Color Gig-E camera which supports some of the above behavioral tests; 10) Promethion high-resolution metabolic phenotyping system. Justification: These purchases will modernize and update an already established phenotyping facility that is no longer has the capacity to support internal demand. It will also add the capability to run multiple tests simultaneously and to test mice and rats in separate equipment located in separate spaces. It will also add the ability to perform more sophisticated tests that refine and enhance our investigators understanding of effects of mTBI and other neurological conditions on cognition, memory and overall behavior, such as the 8-arm maze test and sociability tests; the later has been included because of the growing awareness that difficulties in socialization is a consequence of recurring mTBI. Also added will be the ability to capture in great detail information on metabolism by indirect calorimetry in the animals home cage together with data on activity, body weight and other physiologic parameters. Impact: If funded, this application would give VA researchers at the JJPVA a modernized, cutting-edge rodent phenotyping core with an expanded capability to perform comprehensive behavioral and metabolic phenotyping analysis. to measure at high-resolution the metabolic health of an animal after genetic or experimental These instruments will be used in VA-funded projects in the fields of Alzheimer's disease, traumatic brain injury, and spinal cord injury. The instruments would be a key component to test the efficacy at the whole animal level of many therapeutic interventions and substantially broaden and enhance the understanding of medical conditions that affect the health and quality of life of veterans impacted by these diseases and disabilities.

背景：本应用程序所要求的设备将提供以下能力