ShEEP Request for A Metabolic and Behavioral Phenotyping System

ShEEP 请求建立代谢和行为表型系统

• 批准号: 9795306
• 负责人: CHRISTOPHER P CARDOZO
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795306
• 关键词: Acoustics; Affect; Aging; Alzheimer' s Disease; Animal Behavior; Animal Experimentation; Animal Testing; Animals; Awareness; Behavior; Behavioral; Biomedical Research; Blood Pressure Monitors; Body Weight; Cognition; Color; Computer software; Computers; Core Facility; Data; Diabetes Mellitus; Disease; Engineering; Environment; Equipment; Equipment Malfunction; Evaluation; Funding; Genetic; Health; Home environment; Indirect Calorimetry; Investigation; Knowledge; Laboratories; Learning; Link; Lipids; Martes zibellina; Measurement; Measures; Medical; Medical center; Memory; Metabolic; Metabolic syndrome; Metabolism; Mind; Modality; Modernization; Mus; Neurodegenerative Disorders; Neurologic; Operative Surgical Procedures; Pharmaceutical Preparations; Phenotype; Physical Function; Physiological; Population; Quality of life; Radial; Rattus; Request for Applications; Research; Research Personnel; Research Project Grants; Research Support; Resolution; Rodent; Running; Scientist; Sheep; Shipping; Signal Transduction; Socialization; Spinal cord injury; Stress; System; Testing; Therapeutic Intervention; Training; Trauma; Traumatic Brain Injury; United States National Institutes of Health; Update; Veterans; Work; aging population; arm; behavior test; conditioned fear; diabetes risk; disability; efficacy testing; functional decline; instrument; interest; man; metabolic phenotype; mild traumatic brain injury; morris water maze; prepulse inhibition; programs; repaired; trait

Background: The equipment requested in this application will provide the capability to comprehensively evaluate metabolic and behavioral phenotypes in rats and mice. Our investigators presently use behavioral testing as an endpoint in ongoing, VA-funded research on spinal cord injury (SCI), Alzheimer's disease and mild traumatic brain injury (mTBI). Behavioral testing is also an important endpoint in ongoing NIH funded studies on aging related vulnerability to functional decline after surgery. Because of links between mTBI, Alzheimer's disease or SCI and diabetes and metabolic syndrome, there is an intense interest in having the ability to evaluate metabolic function among many of our investigators. Equipment Requested: This application requests funds to purchase additional equipment needed to update and expand our current rodent phenotyping core: 1) Fear conditioning; 2) Acoustic startle/prepulse inhibition; 3) Open field; 4) Morris water maze; 5) Escape hole radial 8-arm maze; 6) Sociability; 7) Rotorods; 8) Blood pressure monitoring; 9) Ethovision XT13 tracking software and Color Gig-E camera which supports some of the above behavioral tests; 10) Promethion high-resolution metabolic phenotyping system. Justification: These purchases will modernize and update an already established phenotyping facility that is no longer has the capacity to support internal demand. It will also add the capability to run multiple tests simultaneously and to test mice and rats in separate equipment located in separate spaces. It will also add the ability to perform more sophisticated tests that refine and enhance our investigators understanding of effects of mTBI and other neurological conditions on cognition, memory and overall behavior, such as the 8-arm maze test and sociability tests; the later has been included because of the growing awareness that difficulties in socialization is a consequence of recurring mTBI. Also added will be the ability to capture in great detail information on metabolism by indirect calorimetry in the animals home cage together with data on activity, body weight and other physiologic parameters. Impact: If funded, this application would give VA researchers at the JJPVA a modernized, cutting-edge rodent phenotyping core with an expanded capability to perform comprehensive behavioral and metabolic phenotyping analysis. to measure at high-resolution the metabolic health of an animal after genetic or experimental These instruments will be used in VA-funded projects in the fields of Alzheimer's disease, traumatic brain injury, and spinal cord injury. The instruments would be a key component to test the efficacy at the whole animal level of many therapeutic interventions and substantially broaden and enhance the understanding of medical conditions that affect the health and quality of life of veterans impacted by these diseases and disabilities.

背景：本申请中要求的设备将提供以下能力： 综合评估大鼠和小鼠的代谢和行为表型。我们的调查人员目前 使用行为测试作为正在进行的，VA资助的研究脊髓损伤（SCI），阿尔茨海默氏症的终点， 疾病和轻度创伤性脑损伤（mTBI）。行为测试也是正在进行的NIH的一个重要终点 资助了与手术后功能衰退相关的衰老脆弱性研究。由于联系在 mTBI、阿尔茨海默病或SCI以及糖尿病和代谢综合征，人们对具有以下特征的疾病有强烈的兴趣： 评估代谢功能的能力。 申请设备：此申请要求资金购买所需的额外设备， 更新和扩展我们目前的啮齿动物表型核心：1）恐惧条件反射; 2）声音惊吓/前脉冲 抑制; 3）旷场; 4）Morris水迷宫; 5）逃逸孔放射状8臂迷宫; 6）社交性; 7）旋转棒; 8)血压监测; 9）Ethovision XT 13跟踪软件和Color Gig-E摄像头，支持 上述行为测试中的一些; 10）Promethion高分辨率代谢表型系统。 理由：这些采购将使已经建立的表型鉴定设施现代化和更新 它不再有能力支持国内需求。它还将增加运行多个测试的能力 同时，并在位于不同空间的不同设备中测试小鼠和大鼠。它还将添加 能够进行更复杂的测试，完善和提高我们的研究人员对影响的理解， mTBI和其他神经系统疾病对认知、记忆和整体行为的影响，如8臂迷宫 测试和社交能力测试;后者已被包括在内，因为越来越多的人意识到， 社会化是复发性mTBI的结果。还将增加的能力，捕捉在非常详细的 动物饲养笼中通过间接热量测定法获得的代谢信息以及活动、身体 体重和其他生理参数。 影响：如果获得资助，该应用程序将为JJPVA的VA研究人员提供一个现代化的，尖端的 啮齿类动物表型核心，具有扩展的能力， 表型分析以高分辨率测量动物的代谢健康， 这些仪器将用于VA资助的阿尔茨海默病领域的项目， 创伤性脑损伤和脊髓损伤。这些工具将是测试在以下情况下的功效的关键组成部分： 整个动物水平的许多治疗干预，并大大扩大和提高 了解影响退伍军人健康和生活质量的医疗条件， 疾病和残疾。