ShEEP Request for A Metabolic and Behavioral Phenotyping System

ShEEP 请求建立代谢和行为表型系统

• 批准号: 9795306
• 负责人: CHRISTOPHER P CARDOZO
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795306
• 关键词: Acoustics; Affect; Aging; Alzheimer' s Disease; Animal Behavior; Animal Experimentation; Animal Testing; Animals; Awareness; Behavior; Behavioral; Biomedical Research; Blood Pressure Monitors; Body Weight; Cognition; Color; Computer software; Computers; Core Facility; Data; Diabetes Mellitus; Disease; Engineering; Environment; Equipment; Equipment Malfunction; Evaluation; Funding; Genetic; Health; Home environment; Indirect Calorimetry; Investigation; Knowledge; Laboratories; Learning; Link; Lipids; Martes zibellina; Measurement; Measures; Medical; Medical center; Memory; Metabolic; Metabolic syndrome; Metabolism; Mind; Modality; Modernization; Mus; Neurodegenerative Disorders; Neurologic; Operative Surgical Procedures; Pharmaceutical Preparations; Phenotype; Physical Function; Physiological; Population; Quality of life; Radial; Rattus; Request for Applications; Research; Research Personnel; Research Project Grants; Research Support; Resolution; Rodent; Running; Scientist; Sheep; Shipping; Signal Transduction; Socialization; Spinal cord injury; Stress; System; Testing; Therapeutic Intervention; Training; Trauma; Traumatic Brain Injury; United States National Institutes of Health; Update; Veterans; Work; aging population; arm; behavior test; conditioned fear; diabetes risk; disability; efficacy testing; functional decline; instrument; interest; man; metabolic phenotype; mild traumatic brain injury; morris water maze; prepulse inhibition; programs; repaired; trait

Background: The equipment requested in this application will provide the capability to comprehensively evaluate metabolic and behavioral phenotypes in rats and mice. Our investigators presently use behavioral testing as an endpoint in ongoing, VA-funded research on spinal cord injury (SCI), Alzheimer's disease and mild traumatic brain injury (mTBI). Behavioral testing is also an important endpoint in ongoing NIH funded studies on aging related vulnerability to functional decline after surgery. Because of links between mTBI, Alzheimer's disease or SCI and diabetes and metabolic syndrome, there is an intense interest in having the ability to evaluate metabolic function among many of our investigators. Equipment Requested: This application requests funds to purchase additional equipment needed to update and expand our current rodent phenotyping core: 1) Fear conditioning; 2) Acoustic startle/prepulse inhibition; 3) Open field; 4) Morris water maze; 5) Escape hole radial 8-arm maze; 6) Sociability; 7) Rotorods; 8) Blood pressure monitoring; 9) Ethovision XT13 tracking software and Color Gig-E camera which supports some of the above behavioral tests; 10) Promethion high-resolution metabolic phenotyping system. Justification: These purchases will modernize and update an already established phenotyping facility that is no longer has the capacity to support internal demand. It will also add the capability to run multiple tests simultaneously and to test mice and rats in separate equipment located in separate spaces. It will also add the ability to perform more sophisticated tests that refine and enhance our investigators understanding of effects of mTBI and other neurological conditions on cognition, memory and overall behavior, such as the 8-arm maze test and sociability tests; the later has been included because of the growing awareness that difficulties in socialization is a consequence of recurring mTBI. Also added will be the ability to capture in great detail information on metabolism by indirect calorimetry in the animals home cage together with data on activity, body weight and other physiologic parameters. Impact: If funded, this application would give VA researchers at the JJPVA a modernized, cutting-edge rodent phenotyping core with an expanded capability to perform comprehensive behavioral and metabolic phenotyping analysis. to measure at high-resolution the metabolic health of an animal after genetic or experimental These instruments will be used in VA-funded projects in the fields of Alzheimer's disease, traumatic brain injury, and spinal cord injury. The instruments would be a key component to test the efficacy at the whole animal level of many therapeutic interventions and substantially broaden and enhance the understanding of medical conditions that affect the health and quality of life of veterans impacted by these diseases and disabilities.

背景：本申请中要求的设备将提供以下功能 综合评价大鼠和小鼠的代谢和行为表型。我们的调查人员目前 使用行为测试作为正在进行的、由退伍军人事务部资助的脊髓损伤(SCI)、阿尔茨海默氏症研究的终点 疾病和轻度创伤性脑损伤(MTBI)。行为测试也是NIH正在进行的一个重要终点 资助了关于手术后与衰老相关的功能衰退易感性的研究。因为两者之间的联系 MTBI、阿尔茨海默病或脊髓损伤以及糖尿病和代谢综合征，人们对 我们许多研究人员评估代谢功能的能力。 请求的设备：此应用程序请求资金以购买所需的其他设备 更新和扩展我们目前的啮齿动物表型核心：1)恐惧条件作用；2)声学惊吓/预脉冲 抑制；3)开阔场地；4)Morris水迷宫；5)逃逸孔径向8臂迷宫；6)社交；7)机器人； 8)血压监测；9)ethovision XT13跟踪软件和支持以下功能的彩色Gig-E摄像头 上述部分行为学测试；10)灭蚁灵高分辨代谢表型系统。 理由：这些采购将使已经建立的表型鉴定设施现代化和更新 那就是不再有能力支持内需。它还将添加运行多个测试的功能 同时，在位于不同空间的不同设备中测试小鼠和大鼠。它还将添加 能够执行更复杂的测试，以改进和增强我们的调查人员对 MTBI和其他神经疾病对认知、记忆和整体行为的影响，如8臂迷宫 测试和社交能力测试；后者之所以被包括在内，是因为人们越来越意识到 社会化是mTBI反复发生的结果。此外，还将添加捕获详细信息的能力 通过间接量热法了解动物家庭笼子中的新陈代谢信息以及活动、身体的数据 体重和其他生理参数。 影响：如果获得资金，这项应用将为JJPVA的退伍军人管理局研究人员提供一个现代化的、尖端的 具有扩展的执行综合行为和代谢能力的啮齿动物表型核心 表型分析。以高分辨率测量动物在遗传或繁殖后的代谢健康状况 这些实验仪器将用于退伍军人管理局资助的阿尔茨海默病领域的项目， 创伤性脑损伤和脊髓损伤。这些仪器将是测试疗效的关键组件 整个动物水平的许多治疗干预措施，并大大拓宽和提高 了解影响退伍军人健康和生活质量的医疗条件 疾病和残疾。