权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Washington University Chronic KidneyDisease National Resource Center

华盛顿大学慢性肾病国家资源中心

基本信息

批准号：
10747719
负责人：
BENJAMIN D. HUMPHREYS
金额：
$ 90.48万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10747719
关键词：
Acceleration Address Adopted Affect Area Bar Codes Benign Biological Assay CRISPR/Cas technology Cardiovascular system Cell Culture Techniques Cell Nucleus Cells Chronic Chronic Kidney Failure Code Communication Communities Consultations Data Analyses Development Disease Diverse Workforce Doctor of Medicine Doctor of Philosophy Education Equipment Evolution Family Fibrosis Functional disorder Funding Genes Genetic Genetic Techniques Genomic medicine Genus Hippocampus Goals In Vitro Incubators Injury Injury to Kidney Kidney Lead Leadership Metabolic Metabolism Modeling Morbidity - disease rate Mus Pathogenicity Pathologic Patients Population Pre-Clinical Model Proteins Protocols documentation RNA Splicing Radioactive Research Research Personnel Resolution Resource Development Resource Sharing Resources Scientific Advances and Accomplishments Services Students Techniques Technology Testing Time Tissues Tracer Training Tubular formation Underrepresented Minority United States National Institutes of Health Universities Validation Variant Washington Work bioinformatics pipeline biomedical resource computational pipelines cost genetic testing genetic variant hands on instruction improved in silico in situ sequencing in vitro Assay in vivo kidney biopsy kidney metabolism metabolomics mortality multiple omics novel therapeutic intervention oxidation programs recruit response to injury single cell technology stable isotope summer student technological innovation tool transcriptomics variant of unknown significance

项目摘要

Project Summary/Abstract: Overall Component The Washington University Chronic Kidney Disease National Resource Center is focused on the significant problem of chronic kidney disease (CKD), which affects almost 15% of the US population and carries significant morbidity and mortality. Several scientific advances have the promise to accelerate CKD research, but many of these advances are not accessible to the kidney research community due to limited expertise and/or the need for expensive equipment. Thus, this NRC will address the fundamental challenge of providing better access to cutting-edge techniques in single cell omics, genetics, and metabolism to the kidney research community to facilitate advances in CKD research. Dr. Ben Humphreys is the overall program director, and this Center will consist of four Cores: an Administrative Core, two Biomedical Resource Cores, and a Resource Development Core. The Administrative Core will oversee distribution of funds across the Cores, communicate with the National O’Brien Consortium, manage the Summer Student Enrichment Program, and promote the involvement of early stage investigators and a diverse workforce. The Variant Validation Core, one of the Biomedical Resource Cores, investigates the pathogenicity of genetic variants of uncertain significance using CRISPR/Cas9-gene editing, in silico approaches, and tailored in vitro assays. The Metabolism Core, the other Biomedical Resource Core, provides consultation for users and access to a number of metabolic assays to interrogate changes in kidney metabolism relevant to CKD. These assays include Seahorse bioflux analysis, radioactive substrate oxidation assays of tissue ex vivo, untargeted metabolomics, and stable isotope tracer studies. The Metabolism Core will also work with the O’Brien Consortium to provide validated protocols for assays commonly performed (e.g. Seahorse assays on primary cells) and provide hands’ on training using these protocols. The Single Cell Omics Research Evolution (SCORE) Core is the Resource Development Core, which develops protocols and bioinformatics pipelines for cutting-edge techniques like split pool barcoding for single nuclei multi-omics and high resolution in-situ sequencing-based spatially resolved transcriptomics. All four Cores will work together and with the network of O’Brien National Resource Centers to make scientific advancements more accessible to the kidney research community with particular emphasis on junior investigators and development of a diverse biomedical workforce.

项目摘要/摘要：总体构成