Washington University Chronic KidneyDisease National Resource Center

华盛顿大学慢性肾病国家资源中心

• 批准号: 10747719
• 负责人: BENJAMIN D. HUMPHREYS
• 金额: $ 90.48万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10747719
• 关键词: Acceleration; Address; Adopted; Affect; Area; Bar Codes; Benign; Biological Assay; CRISPR/Cas technology; Cardiovascular system; Cell Culture Techniques; Cell Nucleus; Cells; Chronic; Chronic Kidney Failure; Code; Communication; Communities; Consultations; Data Analyses; Development; Disease; Diverse Workforce; Doctor of Medicine; Doctor of Philosophy; Education; Equipment; Evolution; Family; Fibrosis; Functional disorder; Funding; Genes; Genetic; Genetic Techniques; Genomic medicine; Genus Hippocampus; Goals; In Vitro; Incubators; Injury; Injury to Kidney; Kidney; Lead; Leadership; Metabolic; Metabolism; Modeling; Morbidity - disease rate; Mus; Pathogenicity; Pathologic; Patients; Population; Pre-Clinical Model; Proteins; Protocols documentation; RNA Splicing; Radioactive; Research; Research Personnel; Resolution; Resource Development; Resource Sharing; Resources; Scientific Advances and Accomplishments; Services; Students; Techniques; Technology; Testing; Time; Tissues; Tracer; Training; Tubular formation; Underrepresented Minority; United States National Institutes of Health; Universities; Validation; Variant; Washington; Work; bioinformatics pipeline; biomedical resource; computational pipelines; cost; genetic testing; genetic variant; hands on instruction; improved; in silico; in situ sequencing; in vitro Assay; in vivo; kidney biopsy; kidney metabolism; metabolomics; mortality; multiple omics; novel therapeutic intervention; oxidation; programs; recruit; response to injury; single cell technology; stable isotope; summer student; technological innovation; tool; transcriptomics; variant of unknown significance

Project Summary/Abstract: Overall Component The Washington University Chronic Kidney Disease National Resource Center is focused on the significant problem of chronic kidney disease (CKD), which affects almost 15% of the US population and carries significant morbidity and mortality. Several scientific advances have the promise to accelerate CKD research, but many of these advances are not accessible to the kidney research community due to limited expertise and/or the need for expensive equipment. Thus, this NRC will address the fundamental challenge of providing better access to cutting-edge techniques in single cell omics, genetics, and metabolism to the kidney research community to facilitate advances in CKD research. Dr. Ben Humphreys is the overall program director, and this Center will consist of four Cores: an Administrative Core, two Biomedical Resource Cores, and a Resource Development Core. The Administrative Core will oversee distribution of funds across the Cores, communicate with the National O’Brien Consortium, manage the Summer Student Enrichment Program, and promote the involvement of early stage investigators and a diverse workforce. The Variant Validation Core, one of the Biomedical Resource Cores, investigates the pathogenicity of genetic variants of uncertain significance using CRISPR/Cas9-gene editing, in silico approaches, and tailored in vitro assays. The Metabolism Core, the other Biomedical Resource Core, provides consultation for users and access to a number of metabolic assays to interrogate changes in kidney metabolism relevant to CKD. These assays include Seahorse bioflux analysis, radioactive substrate oxidation assays of tissue ex vivo, untargeted metabolomics, and stable isotope tracer studies. The Metabolism Core will also work with the O’Brien Consortium to provide validated protocols for assays commonly performed (e.g. Seahorse assays on primary cells) and provide hands’ on training using these protocols. The Single Cell Omics Research Evolution (SCORE) Core is the Resource Development Core, which develops protocols and bioinformatics pipelines for cutting-edge techniques like split pool barcoding for single nuclei multi-omics and high resolution in-situ sequencing-based spatially resolved transcriptomics. All four Cores will work together and with the network of O’Brien National Resource Centers to make scientific advancements more accessible to the kidney research community with particular emphasis on junior investigators and development of a diverse biomedical workforce.

项目摘要/摘要：总体组成部分 华盛顿大学慢性肾病国家资源中心专注于重要的 慢性肾脏病 (CKD) 问题影响着近 15% 的美国人口，并携带 显着的发病率和死亡率。多项科学进展有望加速 CKD 研究， 但由于专业知识有限，肾脏研究界无法获得其中许多进展 和/或需要昂贵的设备。因此，本 NRC 将解决提供以下服务的根本挑战： 更好地获得单细胞组学、遗传学和肾脏研究代谢方面的尖端技术 社区以促进 CKD 研究的进步。本·汉弗莱斯 (Ben Humphreys) 博士是该项目的总体总监， 中心将由四个核心组成：一个行政核心、两个生物医学资源核心和一个资源核心 开发核心。行政核心将监督核心之间的资金分配，沟通 与国家奥布莱恩联盟合作，管理暑期学生强化计划，并促进 早期研究人员和多元化工作人员的参与。变体验证核心，其中之一 生物医学资源核心，利用技术研究不确定意义的遗传变异的致病性 CRISPR/Cas9 基因编辑、计算机方法和定制的体外测定。新陈代谢核心，另一个 生物医学资源核心，为用户提供咨询并访问多种代谢测定，以 询问与 CKD 相关的肾脏代谢变化。这些测定包括 Seahorse 生物通量分析、 离体组织的放射性底物氧化测定、非靶向代谢组学和稳定同位素示踪剂 研究。代谢核心还将与奥布莱恩联盟合作，提供经过验证的方案 常用的检测（例如原代细胞的海马检测）并提供使用培训 这些协议。单细胞组学研究进展（SCORE）的核心是资源开发 Core，为 split pool 等尖端技术开发协议和生物信息学管道 基于空间分辨的单核多组学条形码和高分辨率原位测序 转录组学。所有四个核心将共同努力并与奥布莱恩国家资源中心网络合作， 使肾脏研究界更容易获得科学进步，特别强调 初级研究人员和多元化生物医学队伍的发展。