ECHO Laboratory Core at Vanderbilt for Integrated Sample Biobanking and Processing

范德堡大学 ECHO 实验室核心，用于集成样本生物库和处理

• 批准号: 10745188
• 负责人: Suman Ranjan Das
• 金额: $ 730万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2030-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10745188
• 关键词: Academic Medical Centers; Address; Architecture; Area; Basic Science; Behavioral; Bioinformatics; Biological; Biological Assay; Biological Markers; Biological Response Modifiers; Blood; Cells; Chemicals; Child Development; Child Health; Childhood; Clinical; Clinical Data; Cloud Computing; Collaborations; Collection; Complex; Cotinine; DNA; Data; Data Analyses; Data Analytics; Development; Diet; Dimensions; Disease; Ensure; Environmental Exposure; Environmental Risk Factor; Epidemiology; Epigenetic Process; Exposure to; Feces; Funding; Genetic; Genomics; Goals; Hair; Health; Human Milk; Infection; Infrastructure; Investments; Isoprostanes; Laboratories; Lead; Leadership; Life; Life Style; Link; Measures; Metadata; Metagenomics; Methods; Mission; Molecular; Nail plate; Nicotine; Nose; Nuclear; Nucleic Acids; Outcome; Participant; Pathogenesis; Patients; Performance; Perinatal; Placenta; Positioning Attribute; Proteomics; Protocols documentation; Public Health; RNA; Research; Research Design; Research Personnel; Resource Sharing; Resources; Risk Factors; Running; Sample Size; Sampling; Science; Secure; Site; System; Tissues; Translational Research; United States National Institutes of Health; Universities; Urine; Validation; analysis pipeline; assay development; biobank; clinical research site; clinical translation; cohort; data analysis pipeline; data exchange; data integration; data management; data sharing; early childhood; early life exposure; experience; high throughput screening; insight; member; metabolomics; microbiome; microbiome research; multiple omics; novel; programs; quality assurance; response; sample collection; social; success; trait; transcriptome sequencing; transcriptomics

SUMMARY Early-life environmental exposures (e.g., social-environmental, parental risk factors, nicotine, diet, infection) are increasingly implicated in the early pathogenesis of childhood diseases that have life-long consequences. Mechanisms linking these exposures to longer-term outcomes remain limited. In 2016, the NIH established the Environmental influences on Child Health Outcomes (ECHO) Program, a collaborative multi-dimensional research initiative to characterize the impact of early-life environmental factors on childhood health (>70 cohorts, >50K participants). While targeted assays within ECHO are likely to lead to disease-specific insight, broad, comprehensive, unbiased assessment of the molecular space for novel discovery—a key mission of ECHO— will necessitate centralization of biobanking efforts/laboratory management with capability for high-throughput “OMICs”/non-“OMICs” assay as well as novel assay development, bioinformatics, and cloud architecture/data sharing for collaborative science. In response to RFA-OD-22-016, Vanderbilt will address this need by establishing the ECHO Laboratory Core at Vanderbilt for Integrated Sample Biobanking and Processing (ELVIS). ELVIS facilitates the collection and processing of biospecimens; manages the biorepository; performs a wide range of biospecimen assays (including novel development), and coordinates metadata and assay data transfer to the Coordinating Center. ELVIS is organized in core “resources” to provide leadership/integration to manage ECHO biobanking, assay performance, and data delivery: (1) administrative/LIMS/biobanking; (2) metabolomics; (3) proteomics; (4) nucleic acid assessment; (5) metagenomics; (6) bioinformatics/study design. We are uniquely positioned for this initiative, leveraging Vanderbilt’s unique long-term strategic investment in functional biobanking and assay: (1) large-scale, reliable biorepository receipt and laboratory management (“LIMS”) capability (>350K patients currently with biospecimens; many other NIH funded biobanks); (2) nationally recognized systems for clinical metadata capture (REDCap, REDBrics; used in NIH initiatives, like All of Us); (3) cutting-edge laboratory cores with capability for novel assay development/validation. We will establish harmonized protocols and workflow for ECHO cohort biospecimen collection and tracking infrastructure from the point of sample collection to long-term storage (Aim 1); perform high-quality, well-powered multi-OMICs and targeted assays to identify molecular correlates of disease trajectories in early life (Aim 2), and provide comprehensive data management platform to facilitate integrated data analysis (Aim 3). ELVIS is an ideal mechanism for ECHO given (1) deep, funded experience in handling the requisite sample sizes in banking and high-throughput assay, including quality assurance measures; (2) prior track record in ECHO to ensure ECHO- specific metadata collection, curation, and harmonization; (3) secure methods for cloud infrastructure for data analysis pipelines and data flow to clinical sites and data analysis center. Successful completion will enable the success of ECHO’s mission to discover molecular underpinnings of early childhood determinants of disease.

概括 生命早期的环境暴露（例如社会环境、父母危险因素、尼古丁、饮食、感染） 越来越多地与具有终生后果的儿童疾病的早期发病机制有关。 将这些风险敞口与长期结果联系起来的机制仍然有限。 2016 年，美国国立卫生研究院 (NIH) 成立了 环境对儿童健康结果的影响 (ECHO) 计划，一个协作的多维项目 研究计划旨在描述生命早期环境因素对儿童健康的影响（>70 个队列， >5 万名参与者）。虽然 ECHO 内的靶向检测可能会导致对疾病特异性的深入了解，但广泛、 对新发现的分子空间进行全面、公正的评估——ECHO 的一项关键使命—— 将需要集中生物样本库工作/具有高通量能力的实验室管理 “OMIC”/非“OMIC”测定以及新颖的测定开发、生物信息学和云架构/数据 共享协作科学。为了响应 RFA-OD-22-016，范德比尔特将通过以下方式满足这一需求： 在范德比尔特建立 ECHO 实验室核心，用于集成样本生物库和处理 （埃尔维斯）。 ELVIS 促进生物样本的收集和处理；管理生物样本库；执行 广泛的生物样本测定（包括新颖的开发），并协调元数据和测定数据 转移到协调中心。 ELVIS 按核心“资源”进行组织，以提供领导力/整合 管理 ECHO 生物样本库、检测性能和数据交付：(1) 管理/LIMS/生物样本库； (2) 代谢组学； (3)蛋白质组学； （四）核酸评估； (5) 宏基因组学； (6)生物信息学/研究设计。 我们在这一举措中处于独特的地位，利用范德比尔特独特的长期战略投资 功能性生物样本库和检测：（1）大规模、可靠的生物样本库接收和实验室管理 （“LIMS”）能力（目前有超过 35 万患者拥有生物样本；许多其他 NIH 资助的生物库）； (2) 全国范围 公认的临床元数据采集系统（REDCap、REDBrics；用于 NIH 计划，如 All of Us）； (3) 具有新型检测开发/验证能力的尖端实验室核心。我们将建立 协调 ECHO 队列生物样本收集和跟踪基础设施的协议和工作流程 样本采集点到长期储存（目标 1）；执行高质量、功能强大的多组学分析 靶向测定，以确定生命早期疾病轨迹的分子相关性（目标 2），并提供 综合数据管理平台，促进综合数据分析（目标3）。 ELVIS 是一个理想的 鉴于 (1) 在处理银行业和银行业所需样本量方面拥有深厚的资金经验，ECHO 的机制 高通量测定，包括质量保证措施； (2) 之前在 ECHO 中的跟踪记录，以确保 ECHO- 具体的元数据收集、管理和协调； (3) 数据云基础设施的安全方法 分析管道和数据流向临床站点和数据分析中心。成功完成将使 ECHO 发现幼儿疾病决定因素的分子基础的使命取得了成功。