Indiana Center for Advanced Renal Microscopy and Molecular Imaging

项目摘要/摘要(中心概述) 自2002年作为NIDDK资助的P50中心成立以来，印第安纳奥布莱恩中心的使命是 对于先进的肾脏显微镜检查已经为肾脏研究人员提供了有效的途径来获得先进的 显微镜的形式。在过去20年的持续资助中，伊利诺伊州奥布莱恩中心开创了 多光子活体显微镜、定量组织的新方法的发展和推广 细胞学和数字图像分析已应用于肾生理和肾脏疾病的联合研究 与来自世界各地的研究人员一起研究病理生理学。使用P30和P50 IU O‘Brien进行的研究 中心已经生成了用于190多种出版物和许多赠款申请的数据，这些数据有助于 开启新一代调查人员的职业生涯。我们期待着继续我们在 新U54机制的联合体框架。为该中心提出的目标和项目如下 组织成三个总体目标-(1)提供小鼠和大鼠的定量活体显微照相作为服务 给肾脏研究人员。提供实践培训，鼓励将活体显微镜作为一种 有效的研究工具。(2)提供肾脏的定量、大规模显微镜检查，为肾脏提供服务 研究人员。三维共聚焦组织细胞术、Codex多重细胞术和空间 转录本将单独或组合提供，支持独特的全面分子 刻画。(3)开发和实施组织透明、光片显微镜和数字技术 图像分析，以扩大肾脏组织的定量显微镜的规模，基于独特的多 尺度/多分辨率策略和最新的数字图像分析方法。 印第安纳州奥布莱恩中心将包括一个管理核心和两个生物医学资源核心-一个 活体显微镜核心和分子成像核心，均由印第安纳生物中心主办 显微镜。就这两个核心有效地扩展印第安纳州奥布莱恩目前提供的服务而言 中心，他们将受益于现有的基础设施、专业知识和过去积累的经验 在该中心工作了20年。该中心还将与以下机构合作，支持资源开发核心 华盛顿大学、内布拉斯加大学和普渡大学的调查人员将开发 将在第二年供资开始时开始实施的技术。

Project Summary/Abstract (Center Overview) From its inception as an NIDDK-funded P50 Center in 2002, the mission of the Indiana O’Brien Center for Advanced Renal Microscopy has been to provide renal researchers with effective access to advanced forms of microscopy. Over the past 20 years of continuous funding, the IU O’Brien Center has pioneered the development and dissemination of novel methods of multiphoton intravital microscopy, quantitative tissue cytometry and digital image analysis that have been applied in collaborative studies of renal physiology and pathophysiology with investigators from around the world. Studies conducted with the P30 and P50 IU O’Brien Center have generated data used in over 190 publications and numerous grant applications that have helped launch the careers of a new generation of investigators. We look forward to continuing our work within the consortium framework of the new U54 mechanism. The goals and projects proposed for the Center are organized into three general aims – (1) Provide quantitative intravital microcopy of mice and rats as a service to renal researchers. Provide hands-on training to encourage dissemination of intravital microscopy as an effective research tool. (2) Provide quantitative, large-scale microscopy of the kidney as a service to renal researchers. Three-dimensional confocal tissue cytometry, CODEX multiplexed cytometry and Spatial Transcriptomics will be offered individually or in combination, supporting uniquely comprehensive molecular characterizations. (3) Develop and implement methods of tissue clearing, light-sheet microscopy and digital image analysis to expand the scale of quantitative microscopy of renal tissues, based upon a unique multi- scale/multi-resolution strategy and state-of-the-art methods of digital image analysis. The Indiana O’Brien Center will include an Administrative Core, and two Biomedical Resource cores - an Intravital Microscopy Core, and a Molecular Imaging Core, both hosted by the Indiana Center for Biological Microscopy. Insofar as these two cores effectively extend services currently offered by the Indiana O’Brien Center, they will benefit from the existing infrastructure, expertise and experience developed over the past twenty years by the Center. The Center will also support a Resource Development Core, in collaboration with investigators at the University of Washington, University of Nebraska and Purdue University that will develop technologies that will start to be implemented at the beginning of the second year of funding.