Indiana Center for Advanced Renal Microscopy and Molecular Imaging

项目概要/摘要（中心概述） 自2002年作为NIDDK资助的P50中心成立以来，印第安纳州奥布莱恩中心的使命 先进的肾显微镜一直为肾脏研究人员提供有效的先进的 显微镜的形式。在过去20年的持续资助中，IU奥布莱恩中心开创了 多光子活体显微镜新方法的开发和推广，定量组织 细胞计数和数字图像分析已应用于肾脏生理学的合作研究， 与来自世界各地的研究人员共同研究病理生理学。使用P30和P50 IU奥布莱恩进行的研究 中心已经产生了190多个出版物和众多资助申请中使用的数据， 开启新一代调查员的职业生涯我们期待着在联合国系统内继续我们的工作。 新的U 54机制的联合体框架。为该中心提出的目标和项目是 组织成三个总的目标-（1）提供小鼠和大鼠的定量活体显微镜检查作为服务 肾脏研究人员。提供实践培训，鼓励传播活体显微镜检查， 有效的研究工具。(2)提供定量、大规模的肾脏显微镜检查， 研究人员三维共聚焦组织细胞术、CODEX多重细胞术和Spatial 转录组学将单独或组合提供，支持独特的全面的分子生物学。 特征化。(3)制定和实施组织清理、光片显微镜和数字化方法 图像分析，以扩大规模的定量显微镜的肾组织，基于一个独特的多， 尺度/多分辨率策略和最先进的数字图像分析方法。 印第安纳州奥布莱恩中心将包括一个行政核心和两个生物医学资源核心-一个 活体显微镜核心和分子成像核心，两者都由印第安纳州生物医学中心主办。 显微镜尽管这两个核心有效地扩展了印第安纳州奥布莱恩目前提供的服务 中心，他们将受益于现有的基础设施，专业知识和经验，在过去的发展 20年来，中心。该中心还将支持一个资源开发核心， 华盛顿大学、内布拉斯加大学和普渡大学的研究人员将开发 这些技术将在第二个供资年度开始时开始实施。

Project Summary/Abstract (Center Overview) From its inception as an NIDDK-funded P50 Center in 2002, the mission of the Indiana O’Brien Center for Advanced Renal Microscopy has been to provide renal researchers with effective access to advanced forms of microscopy. Over the past 20 years of continuous funding, the IU O’Brien Center has pioneered the development and dissemination of novel methods of multiphoton intravital microscopy, quantitative tissue cytometry and digital image analysis that have been applied in collaborative studies of renal physiology and pathophysiology with investigators from around the world. Studies conducted with the P30 and P50 IU O’Brien Center have generated data used in over 190 publications and numerous grant applications that have helped launch the careers of a new generation of investigators. We look forward to continuing our work within the consortium framework of the new U54 mechanism. The goals and projects proposed for the Center are organized into three general aims – (1) Provide quantitative intravital microcopy of mice and rats as a service to renal researchers. Provide hands-on training to encourage dissemination of intravital microscopy as an effective research tool. (2) Provide quantitative, large-scale microscopy of the kidney as a service to renal researchers. Three-dimensional confocal tissue cytometry, CODEX multiplexed cytometry and Spatial Transcriptomics will be offered individually or in combination, supporting uniquely comprehensive molecular characterizations. (3) Develop and implement methods of tissue clearing, light-sheet microscopy and digital image analysis to expand the scale of quantitative microscopy of renal tissues, based upon a unique multi- scale/multi-resolution strategy and state-of-the-art methods of digital image analysis. The Indiana O’Brien Center will include an Administrative Core, and two Biomedical Resource cores - an Intravital Microscopy Core, and a Molecular Imaging Core, both hosted by the Indiana Center for Biological Microscopy. Insofar as these two cores effectively extend services currently offered by the Indiana O’Brien Center, they will benefit from the existing infrastructure, expertise and experience developed over the past twenty years by the Center. The Center will also support a Resource Development Core, in collaboration with investigators at the University of Washington, University of Nebraska and Purdue University that will develop technologies that will start to be implemented at the beginning of the second year of funding.