Functional genomics resources for the Drosophila - TR&D3

果蝇功能基因组学资源 - TR

• 批准号: 10620331
• 负责人: JONATHAN D ZIRIN
• 金额: $ 32.27万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10620331
• 关键词: Address; Animals; Antibodies; Area; Base Pairing; Binding Proteins; Biological Assay; Biological Models; Biotin; Biotinylation; Boston; Cell Culture Techniques; Cell Line; Cells; Cellular biology; Communication; Communities; Complement; Coupled; Culture Media; Detection; Development; Disease; Distant; Drosophila Proteins; Drosophila genus; Emerging Technologies; Enzymes; Fab Immunoglobulins; Fusion Protein Expression; Genes; Genetic; Health; Image; Imaging Device; Interest Group; Label; Letters; Libraries; Ligase; Location; Love; Maps; Mass Spectrum Analysis; Methods; Molecular Genetics; Molecular Probes; Neurons; Nucleic Acids; Organ; Organelles; Peroxidases; Protein Secretion; Proteins; Proteome; Proteomics; Reagent; Regulation; Research; Research Design; Sampling; Signal Transduction; System; Technology; Testing; Tissues; Transcriptional Regulation; Visualization; Yeasts; cell type; chromatin immunoprecipitation; functional genomics; genetic technology; genome resource; improved; in vivo; innovation; interest; medical schools; nanobodies; new technology; next generation sequencing; protein complex; protein function; protein purification; response; screening; technology research and development; tool

PROJECT SUMMARY – TR&D3 Development of protein technologies for use in vivo in the Drosophila system has lagged behind development of molecular genetic technologies. In this project, we will develop protein-based technologies that complement and extend the type of studies that can be done in this exemplary model system. We will focus on development of proximity labeling, as facilitated by peroxidases or biotin ligases, as tools for in vivo proteomics analysis of specific subcellular compartments and signaling. We will additionally develop a robust pipeline for isolation of nanobodies that can be used in diverse applications, including for in vivo expression of fusion proteins that facilitate visualization and functional blocking. Collaborators with interests in using the technologies to study cell biology, development, transcription control, and neuronal networks provide appropriate assays for iterative testing and improvement. Altogether, this project will help fill an important gap in the Drosophila toolbox that can help provide a more complete picture of functions at the subcellular, cellular, organ, and whole animal levels that would not be achievable in cell systems or using only molecular genetic tools.

项目总结--tr&d3 用于果蝇体内系统的蛋白质技术的开发滞后于开发 分子基因技术。在这个项目中，我们将开发基于蛋白质的技术，以补充 并扩展可以在该示例性模型系统中进行的研究的类型。我们将以发展为中心。 由过氧化物酶或生物素连接酶促进的邻近标记作为体内蛋白质组学分析的工具 特定的亚细胞隔间和信号。我们还将开发一条强大的管道来分离 可用于多种应用的纳米体，包括用于体内表达融合蛋白的纳米体 便于可视化和功能阻止。有兴趣使用这些技术进行研究的合作者 细胞生物学、发育、转录调控和神经元网络为迭代分析提供了合适的分析方法 测试和改进。总之，这个项目将帮助填补果蝇工具箱中的一个重要空白， 可以帮助提供更完整的亚细胞、细胞、器官和整个动物的功能图景 这是在细胞系统中或仅使用分子遗传工具无法达到的水平。