Core A-Pathology Core

核心A（病理学核心）-摘要/总结 胰腺导管腺癌（PDAC）的研究提出了独特的挑战， 复杂的组织病理学，肿瘤微环境（TME）的许多不同的细胞类型，无数的遗传 异常和相关的生物学影响，以及组织可用性和质量的实际限制。我们 病理学团队凭借其在小鼠和人类PDAC方面的深厚专业知识，在以下方面发挥了核心和重要作用： 从P01开始的几乎所有实验的病理学分析。一般来说，这些 活动包括评价遗传工程小鼠（GEM），包括具有时间调节的小鼠， 上皮和/或基质成分中的区室特异性破坏;肿瘤促进信号传导的分析 细胞类型特异性定位于肿瘤细胞本身或在宿主反应中的途径， TME; TME内细胞成分的识别和多维评估;以及跨 GEM模型和人类肿瘤样本之间观察结果的种属验证。病理学核心将 继续收集、维护、存档和记录所有人类和小鼠PDAC相关生物资源。 这些材料包括由原发性和转移性人类和小鼠PDAC产生的类器官，以及 注释的低传代PDAC细胞系的储存库。核心A将与项目密切合作 研究者要实现三个具体目标：i）提供组织学服务和咨询专业知识，包括 集中审查小鼠和人胰腺肿瘤的病理学评价; ii）提供 各种免疫组织化学、免疫荧光和原位免疫组织化学的基础设施和技术专长 利用定量图像分析的杂交测定;和iii）产生、表征和维持类器官 模型，以及供项目使用的早期传代肿瘤和基质细胞培养物， 建模与实验治疗学核心。这个核心的进步的证明来自于 在前一个周期中产生>165个PDX和>30个低传代PDAC细胞系。核心A的领导者是 博士MDACC的Huamin Wang，与共同研究者Anirban Maitra博士和Michael Kim博士一起-带来长期- 长期的专业知识，众多的P01合作和在组织病理学和 人和鼠PDAC组织的分子评估。核心A将建立在先前的强大基础之上 以及研究者之间的许多持续互动，以促进非常成功的P01结局。

Core A (Pathology Core) - Abstract/Summary The study of pancreatic ductal adenocarcinoma (PDAC) presents unique challenges given its formidably complex histopathology, the many diverse cell types of the tumor microenvirionment (TME), myriad genetic abnormalities and associated biological impact, and practical limitations of tissue availability and quality. Our pathology team, with its deep expertise in mouse and human PDAC, has played a central and essential role in the pathologic analysis of virtually all experiments of this P01 since its inception. Broadly speaking, these activities include evaluation of genetically engineered mice (GEM), including mice with temporally regulated, compartment-specific disruption in epithelial and / or stromal components; analyses of tumor promoting signaling pathways with cell-type specific localization to either neoplastic cells per se, or within the host response in the TME; identification and multi-dimensional assessments of cellular components within the TME; and the cross- species validation of observations between GEM models and human tumor samples. The Pathology Core will continue to collect, maintain, archive and record all human and mouse PDAC-associated biological resources. These materials include organoids created from primary and metastatic human and mouse PDAC, and a repository of annotated low-passage PDAC cell lines. Core A will work in close collaboration with the Project Investigators to achieve three specific aims: i) to provide histology services and consultative expertise, including centralized review in the pathologic evaluation of mouse and human pancreatic neoplasms; ii) to provide infrastructure and technical expertise for a variety of immunohistochemical, immunofluorescent and in situ hybridization assays with quantitative image analysis; and iii) to generate, characterize and maintain organoid models, as well as early passage tumor and stromal cells cultures for use by the Projects as well as for allele engineering in The Modeling & Experimental Therapeutics Core. A testament to this Core’s progress stems from the creation of >165 PDXs and >30 low passage PDAC cell lines during the previous cycle. Core A’s leader is Dr. Huamin Wang at MDACC, joined by Co-Investigators Drs. Anirban Maitra and Michael Kim- bring long- standing expertise, numerous P01 collaborations and a strong publication record in the histopathological and molecular assessment of human and murine PDAC tissues. Core A will build upon the strong foundation of prior and many ongoing interactions between investigators in order to facilitate a highly successful P01 outcome.