Computational Core: Biostatistical Analysis and Network Modelling

计算核心：生物统计分析和网络建模

• 批准号: 10622459
• 负责人: David Gjertson
• 金额: $ 21.47万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10622459
• 关键词: Adopted; Allogenic; Animals; Architecture; Area; Automobile Driving; Biological Assay; Biometry; California; Cells; Clinical; Clinical Research; Complex; Computer Analysis; Computer Models; Data; Data Analyses; Data Set; Databases; Development; Dimensions; Discriminant Analysis; Disease; Ensure; Face; Fire - disasters; Future; Generations; Genes; Goals; Health Insurance Portability and Accountability Act; Hepatic; Histopathology; Human; Image; Immune; Immune response; Immunological Models; Immunophenotyping; Injury; Ischemia; Los Angeles; Machine Learning; Manuscripts; Meditation; Methodology; Methods; Modeling; Molecular; Monitor; Mus; Myeloid Cells; Noise; Outcome; Pathologic; Pathway Analysis; Pathway interactions; Phase; Preparation; Procedures; Process; Proteins; Reperfusion Injury; Reporting; Research; Research Personnel; Research Project Grants; Resource Sharing; Role; Sampling; Secure; Services; Signal Transduction; Site; System; Training; Transplantation; Universities; Visualization; Water; clinically relevant; cloud based; complex data; cost; data infrastructure; data management; data modeling; data repository; deep learning; design; digital; experimental study; generative adversarial network; heterogenous data; immunoregulation; improved; interest; learning community; liver ischemia; liver transplantation; longitudinal analysis; machine learning method; machine vision; mouse model; multimodal data; network models; neural network; novel; phenotypic data; preclinical study; predictive modeling; programs; repository; response; tool; transcriptome; transcriptome sequencing; transcriptomics; trend

PROJECT SUMMARY This application proposes three research Projects and three Cores for shared resources initiated and led by investigators at the University of California Los Angeles (UCLA), centered around the theme of investigating innate-adaptive immunoregulation in liver transplant ischemia/reperfusion Injury (IRI). Projects 1 and 2 focus on IRI meditation of immune responses following hepatic grafting in syngeneic and allogeneic murine models, respectively; whereas, Project 3 concentrates on elucidating the role of IRI on immunoregulation following human OLT. The Computational Core provides data management, biostatistical and computational expertise required for the proper analysis and network modelling of data generated by Projects. Modelling will allow the integration of findings from the three types of liver transplants. Services will be provided in the following areas:  Data monitoring and management.  A central infrastructure for data preprocessing of high throughput molecular data.  Biostatistical design, analysis and interpretation of project studies.  Statistical monitoring of studies to ensure interpretability of results.  Development of biostatistical methodology for statistical problems in these projects.  Modeling the immune-cell dynamics to interpret immune-phenotyping datasets.  Process digital histopathology images using a deep learning approach called generative adversarial networks (GAN) to aid in computational/digital quantification and prediction of expressed proteins and spatially resolved genes at pathologic features of interest.  Integrate molecular and cellular scale data and analyses using matrix factorization and tensor decomposition.  Assistance with manuscript preparation.

项目总结 该申请提出了三个研究项目和三个核心共享资源，由 加州大学洛杉矶分校(UCLA)的调查人员，围绕调查的主题 肝移植缺血再灌注损伤中的先天适应性免疫调节项目1和2的重点是 同种和异基因小鼠肝移植后免疫反应的IRI冥想， 然而，项目3集中于阐明IRI在以下免疫调节中的作用 人体原位肝移植。计算核心提供数据管理、生物统计和计算专业知识 需要对项目产生的数据进行适当的分析和网络建模。建模将允许 整合三种类型的肝移植的研究结果。我们将在以下方面提供服务： 数据监控和管理。 是对高通量分子数据进行数据预处理的中央基础设施。 项目研究的生物统计设计、分析和解释。 对研究进行统计监测，以确保结果的可解释性。 为这些项目中的统计问题开发生物统计学方法。 对免疫细胞动力学进行建模以解释免疫表型数据集。 使用称为生成性对抗的深度学习方法处理数字组织病理学图像 网络(GAN)帮助计算/数字量化和预测表达的蛋白质和 在感兴趣的病理特征上空间分辨的基因。 集成了分子和细胞尺度的数据，并使用矩阵因式分解和张量分解进行分析。 协助手稿准备工作。