Project 2: Aquatic Cyanobacteria Plant Collections
项目2:水生蓝藻植物收藏
基本信息
- 批准号:10621871
- 负责人:
- 金额:$ 31.58万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-07-01 至 2025-04-30
- 项目状态:未结题
- 来源:
- 关键词:AnabolismAntineoplastic AgentsBiologicalBiological AssayBiological TestingBotanicalsChemicalsChemistryChicagoCollectionCountryCyanobacteriumDiseaseDrug KineticsDrynessElementsEvaluationFormulationFractionationFresh WaterGene ClusterGenomeGenomicsGoalsGreat Lakes RegionHealthHeat-Shock ResponseHumanIllinoisIn VitroIndividualInvestigationLaosLeadLibrariesLightMalignant NeoplasmsMetabolismMidwestern United StatesMiningMonitorNatural ProductsNatural SourceNorth CarolinaOhioPathway interactionsPharmacy facilityPhytochemicalPlantsProductionProtocols documentationProviderPuerto RicoResearchResearch ActivitySamplingSolubilitySourceStructureSystemTaxonomyTestingTherapeuticUniversitiesWeightWorkanti-cancercollegedrug developmentexperienceimprovedin vivonovelnovel anticancer drugprogramsstressor
项目摘要
The overarching goal of this Program Project proposal is to discover natural products that can serve as
anticancer drug leads from diverse natural sources. Project 2, located at the College of Pharmacy, University of
Illinois at Chicago (UIC), is comprised of botanical, chemical and biological elements. The primary goals for
Project 2 are the discovery of new lead anticancer compounds from cyanobacteria and providing tropical plant
material for Project 1, housed at The Ohio State University (OSU). The underlying hypothesis for Project 2
is that the diverse natural product structures from cultured non-marine cyanobacteria and tropical plants
will be a rich source for anticancer leads. To achieve the stated goals the specific aims for Project 2 are as
follows: 1) to obtain, culture, extract, and prepare fractions of cyanobacterial strains, and to sequence
the genome of selected strains. We will obtain 100 cyanobacterial strains per year. The strain collection will
focus on samples collected in the Upper Midwest and Great Lakes regions of the U.S. Each strain will be cultured,
extracted, fractionated, and provided for biological evaluation in the biological test systems available at Core 1
and Project 1 as well as our external providers of bioassays. As a complementary and integrated approach, we
will sequence and genome mine the genomes of 20 strains per year and strains will be selected for sequencing
based on a) promising biological activity; and b) prioritization based on chemotaxonomy or PCR screen. 2) To
isolate and determine the structures of active cyanobacterial metabolites, and to improve yields of lead
compounds. Extracts showing promising activity will be fractionated using activity-monitored fractionation to
obtain pure natural product(s). The structurally characterized, active isolates will be extensively evaluated in the
biological test systems available to the program. We will re-isolate larger amounts of any candidate compound.
In order to improve yields, when necessary, we will evaluate different cultivation conditions, and explore
heterologous expression of the biosynthetic gene cluster of lead compounds, which will serve to provide an
alternative source for production. 3) To acquire all plant materials that will be required by the proposed
Program Project. We will obtain 150 fully documented and identified plant samples per year from selected
countries of the world. The dried and milled plant material will be provided to Project 1 for further processing.
We will also re-collect plant material for larger-scale isolation of active compounds requiring more detailed
biological evaluation as needed.
本计划项目提案的总体目标是发现可以作为
抗癌药物的来源多种多样。项目2,位于药学院,大学
伊利诺伊州芝加哥大学(UIC),是由植物,化学和生物元素。的主要目标
项目二是从蓝藻中发现新的抗癌先导化合物,并为热带植物提供
项目1的材料,位于俄亥俄州州立大学(OSU)。项目2的基本假设
从培养的非海洋蓝细菌和热带植物中获得的多种天然产物结构
将是抗癌药物的丰富来源为实现既定目标,项目2的具体目标如下
如下:1)获得、培养、提取和制备蓝藻菌株的级分,并测序
所选菌株的基因组。我们每年将获得100株蓝藻菌株。菌株收集将
重点关注在美国上中西部和五大湖地区采集的样本。将培养每种菌株,
提取、分离并提供用于核心1可用生物试验系统中的生物学评价
和项目1以及我们的外部生物测定供应商。作为一种补充和综合办法,我们
每年将对20个菌株进行测序和基因组挖掘,并选择菌株进行测序
基于a)有希望的生物活性;和B)基于化学分类学或PCR筛选的优先化。2)到
分离和确定活性蓝藻代谢产物的结构,提高铅的产率
化合物.将使用活性监测分馏对显示有希望活性的提取物进行分馏,
获得纯天然产物。结构特征的活性分离株将在
生物学测试系统。我们将重新分离出大量的候选化合物。
为了提高产量,必要时,我们将评估不同的栽培条件,并探索
先导化合物的生物合成基因簇的异源表达,这将有助于提供一种
生产的替代来源。3)获得所有的植物材料,将需要由拟议的
计划项目。我们每年将从选定的植物中获得150个有充分记录和鉴定的植物样本。
世界各国。干燥和研磨的植物材料将提供给项目1进行进一步加工。
我们还将重新收集植物材料,以便更大规模地分离需要更详细研究的活性化合物。
根据需要进行生物学评价。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JIMMY ORJALA其他文献
JIMMY ORJALA的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JIMMY ORJALA', 18)}}的其他基金
Research Training in Natural Product Complementary and Integrative Health
天然产品补充和综合健康研究培训
- 批准号:
10066308 - 财政年份:2013
- 资助金额:
$ 31.58万 - 项目类别:
Research Training in Natural Product Complementary and Integrative Health
天然产品补充和综合健康研究培训
- 批准号:
10543771 - 财政年份:2013
- 资助金额:
$ 31.58万 - 项目类别:
Research Training in Natural Product Complementary and Integrative Health
天然产品补充和综合健康研究培训
- 批准号:
10320738 - 财政年份:2013
- 资助金额:
$ 31.58万 - 项目类别:
Cultured Cyanobacteria, Isolation Chemistry, Structure Elucudation, and Plant Acq
培养蓝藻、分离化学、结构解析和植物采集
- 批准号:
7302062 - 财政年份:2007
- 资助金额:
$ 31.58万 - 项目类别:
Project 2: Aquatic Cyanobacteria Plant Collections
项目2:水生蓝藻植物收藏
- 批准号:
10165646 - 财政年份:2007
- 资助金额:
$ 31.58万 - 项目类别:
Project 2: Aquatic Cyanobacteria Plant Collections
项目2:水生蓝藻植物收藏
- 批准号:
10410425 - 财政年份:2007
- 资助金额:
$ 31.58万 - 项目类别:
Microscale LC-MS and NMR Methodology:Rapid Natural (RMI)
微型 LC-MS 和 NMR 方法:Rapid Natural (RMI)
- 批准号:
7011315 - 财政年份:2005
- 资助金额:
$ 31.58万 - 项目类别:
Microscale LC-MS and NMR Methodology:Rapid Natural (RMI)
微型 LC-MS 和 NMR 方法:Rapid Natural (RMI)
- 批准号:
7270499 - 财政年份:2005
- 资助金额:
$ 31.58万 - 项目类别:
Microscale LC-MS and NMR Methodology for Rapid Natural *
Rapid Natural 的微型 LC-MS 和 NMR 方法 *
- 批准号:
7125188 - 财政年份:2005
- 资助金额:
$ 31.58万 - 项目类别:
Cultured Cyanobacteria, Isolation Chemistry, Structure Elucudation, and Plant Acq
培养蓝藻、分离化学、结构解析和植物采集
- 批准号:
7921382 - 财政年份:
- 资助金额:
$ 31.58万 - 项目类别:
相似海外基金
Delays in Acquisition of Oral Antineoplastic Agents
口服抗肿瘤药物的获取延迟
- 批准号:
9975367 - 财政年份:2020
- 资助金额:
$ 31.58万 - 项目类别:
Eliminate the difficulty of venous puncture in patients receiving antineoplastic agents - Development of a new strategy for the prevention of induration-
消除接受抗肿瘤药物的患者静脉穿刺的困难 - 制定预防硬结的新策略 -
- 批准号:
16K11932 - 财政年份:2016
- 资助金额:
$ 31.58万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular mechanisms of the antineoplastic agents inhibiting DNA replication and their applications to cancer patient treatmen
抗肿瘤药物抑制DNA复制的分子机制及其在癌症患者治疗中的应用
- 批准号:
19591274 - 财政年份:2007
- 资助金额:
$ 31.58万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
PNET EXPERIMENTAL THERAPEUTICS--ANTINEOPLASTIC AGENTS AND TREATMENT DELIVERY
PNET 实验治疗——抗肿瘤药物和治疗实施
- 批准号:
6346309 - 财政年份:2000
- 资助金额:
$ 31.58万 - 项目类别:
TYROSINE KINASE INHIBITORS AS ANTINEOPLASTIC AGENTS
酪氨酸激酶抑制剂作为抗肿瘤剂
- 批准号:
2885074 - 财政年份:1999
- 资助金额:
$ 31.58万 - 项目类别:
TYROSINE KINASE INHIBITORS AS ANTINEOPLASTIC AGENTS
酪氨酸激酶抑制剂作为抗肿瘤剂
- 批准号:
6174221 - 财政年份:1999
- 资助金额:
$ 31.58万 - 项目类别: