University of Kansas Alzheimers Disease Center P30 Neuropathology supplement

堪萨斯大学阿尔茨海默病中心 P30 神经病理学补充

基本信息

批准号：
10741516
负责人：
RUSSELL H. SWERDLOW
金额：
$ 33.66万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-15 至 2026-06-30
项目状态：
未结题

项目摘要

PROJECT SUMMARY This Supplement requests one year of funding to support unforeseen costs associated with the University of Kansas Alzheimer’s Disease Research Center (KU ADRC) Neuropathology (NP) Core. The Neuropathology Core provides critical KU ADRC infrastructure in collaborating with other ADRC Cores (Figure 1). The neuropathology core function is essential to the Center’s mission and scientific theme by 1) Maintaining the autopsy program and Brain Bank; 2) Providing neuropathologic diagnosis, consultation, and training for investigators and trainees; 3) Obtaining dural and skin biopsies for induced pluripotent stem cell (IPSC) lines; and 4) Providing brain tissue for mitochondrial DNA (mtDNA) sequencing by the Biomarker Core. The NP Core continually upgrades it status to the diagnostic advances in the field and evolves according to the approved and accepted new guidelines, knowledge and state-of-the-art technology. Furthermore, the Core also participates in the important debates that produce these advances. An unanticipated growth in the number of investigators requesting NP Core support, an emergent unforeseen condition limiting our core to create a digital library of glass slides, increasing request for postmortem CSF specimens and an unpredicted obsolescence of our essential equipment to be replaced, predicates this request. We proposed in our recently funded P30 to provide open libraries of whole-slide scanned images to computer scientists and other investigators utilizing the scanners located in the department of pathology. However, due to increasing demand for neoplastic and other pathology specimens in the department, we have not been able to use this service properly as the current scanner is slow and the department cannot allocate proper time needed for our “research” projects. On the other hand, we have started working with computer scientists in different centers to utilize artificial intelligence (AI)/machine learning to quantify pathologic findings such as amyloid plaques and tangles in neurodegenerative diseases. However, the lack of access to a whole-slide scanner has incumbered our progress. Meanwhile, as the expectation was created, now we have increasing demand for a service we are unable to properly deliver and did not include its cost in submitted P30 budget. Secondly, we realize a need to extend our effort to collect postmortem cerebrospinal fluid to address increasing demand for this valuable specimen and assist our biomarker core for their biomarker studies. Neuropathology Core activities continue to rapidly grow, requiring a more comprehensive formal database program to support a higher level of Core documentation and inventory performance. Finally, we underestimated the rate of degeneration in our essential equipment such as tissue processor and microtome and their lack of extended technical support from their manufacturers for the coming years. For sustainable growth, we must replace them with a new reliable machine to be functional and supported for at least the next 10 years.

项目摘要这种补充要求一年的资金支持与大学相关的不可预见的费用堪萨斯州阿尔茨海默氏病研究中心（KU ADRC）神经病理学（NP）核心。神经病理学核心与其他ADRC核心合作提供了关键的KU ADRC基础架构（图1）。神经病理学核心功能对于中心的使命和科学主题至关重要。维护尸检计划和大脑库； 2）提供神经病理学诊断，咨询和对调查人员和学员的培训； 3）获得用于诱导多能干细胞的硬脑膜和皮肤活检（IPSC）线； 4）提供了生物标志物核心线粒体DNA（mtDNA）测序的脑组织。 NP核心将其状态不断地升级到该领域的诊断进展，并根据已批准并接受了新的准则，知识和最先进的技术。此外，核心也参加产生这些进步的重要辩论。要求NP核心支持的调查人员数量意外增长，这是一个不可预见的条件限制了我们的核心来创建载玻片的数字库，增加了对验尸CSF的要求该请求预测，要替换的基本设备的标本和不可预测的过时。我们在最近资助的P30中提议，为计算机提供全面扫描图像的开放库科学家和其他研究人员利用位于病理学系的扫描仪。但是，由于对部门中对肿瘤和其他病理标本的需求增加，我们无法正确使用此服务，因为当前的扫描仪很慢，并且部门无法分配适当的时间对于我们的“研究”项目。另一方面，我们已经开始与不同的计算机科学家合作利用人工智能（AI）/机器学习来量化病理发现，例如淀粉样蛋白神经退行性疾病中的斑块和缠结。但是，缺乏进入全扫描仪的访问同时，随着期望的创造，我们对一个人的需求不断增加服务我们无法正确交付，并且没有将其成本包括在提交的P30预算中。其次，我们意识到需要扩展我们为收集死后脑脊液的努力，以满足对日益增长的需求这个有价值的标本并为我们的生物标志物核心提供生物标志物研究。神经病理学核心活动继续迅速增长，需要更全面的正式数据库支持更高级别的核心文档和库存性能的程序。最后，我们低估了我们的基本设备的变性速率，例如组织处理器和微型群，缺乏在未来几年中，他们的制造商提供了技术支持。为了可持续增长，我们必须将它们替换为新的可靠机器，以便至少在接下来的10年中得到支持。

项目成果

期刊论文数量（69）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Sex Differences in Resilience and Resistance to Brain Pathology and Dysfunction Moderated by Cerebrovascular Response to Exercise and Genetic Risk for Alzheimer's Disease.

DOI：
10.3233/jad-220359
发表时间：
2022
期刊：
JOURNAL OF ALZHEIMERS DISEASE
影响因子：
4
作者：
Palmer, Jacqueline A.;Kaufman, Carolyn S.;Vidoni, Eric D.;Honea, Robyn A.;Burns, Jeffrey M.;Billinger, Sandra A.
通讯作者：
Billinger, Sandra A.

Ease of use, feasibility and inter-rater reliability of the refined Cue Utilization and Engagement in Dementia (CUED) mealtime video-coding scheme.