National Resource for Aplysia

海兔国家资源

• 批准号: 10591420
• 负责人: MICHAEL C SCHMALE
• 金额: $ 56.55万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10591420
• 关键词: Adult; Affect; Age; Aging; Animals; Aplysia; Aplysia species; Applied Research; Behavior; Biological Models; Biomedical Research; Brain Hypoxia-Ischemia; California; Caloric Restriction; Characteristics; Communities; Complex; DNA; Data; Development; Diet; Disease; Education; Environment; Ganglia; Gene Expression; Genetic Transcription; Glycolysis; Goals; Harvest; Health; Hypoxia; Injury; Laboratory Research; Learning; Life; Life Cycle Stages; Longevity; Malnutrition; Memory; Metabolic; Mission; Mitochondria; Modeling; Molecular; Monitor; Nervous System; Nervous System Physiology; Nervous System Trauma; Neurons; Pathway interactions; Pattern; Phase; Phenotype; Photoperiod; Physiological; Physiology; Play; Production; Protocols documentation; RNA; Reflex action; Reperfusion Injury; Research; Research Personnel; Research Project Grants; Resistance; Resources; Role; Sales; Sampling; Services; Ships; Siblings; Snails; Stroke; Study models; Syndrome; Temperature; Time; Tissues; Universities; aged; cell injury; cohort; coping mechanism; cost; egg; experimental study; human disease; human subject; improved; insight; ischemic injury; marine; neurophysiology; novel; programs; reproductive; resilience; resistance mechanism; screening; senescence; stroke model; transcriptome sequencing; transcriptomics; water quality

The mission of the National Resource for Aplysia at the University of Miami (the Resource) is to produce and distribute consistently high quality cultured sea hares, Aplysia californica, for biomedical research and education and to improve the usefulness of this model system by applied research. Aplysia have played a vital role in fundamental discoveries of how cellular and molecular changes in the nervous system underlie basic behaviors, learning and memory due to unique characteristics of their simple nervous system that provide an ideal model system for such studies. The two aims of the proposed continuation of this resource program are: 1. Production and Distribution – The Resource will continue to culture A. californica and make all life stages, from eggs to large adults, available to researchers at a reasonable cost. We anticipate sales will continue at a level of 10,000 – 15,000 animals per year. We will also accommodate requests by users for separately reared, same-aged cohorts, specialized diets, or harvesting of specific tissues for RNA or DNA. In addition, we will make available mature and senescent stage animals, validated using criteria generated by the Resource, as well as additional specialized animals as developed in our planned Applied Research programs. 2. Applied Research Programs to support the Resource – We will conduct two applied research projects to create new Aplysia resources that we believe will expand the usefulness of this model system to researchers: 2.1 Molecular characterization of the caloric restriction model of arrested aging – We will investigate the mechanisms involved in lengthening the one year Aplysia lifecycle via caloric restriction (CR). We will document the portions of the life cycle such as pre-reproductive, mature and aged that are prolonged by CR. We will also examine alterations in previously identified gene expression profiles associated with aging at these stages, and determine how they are affected by CR. This project will expand the usefulness of these animals as models of aging. 2.2 Molecular characterization of the hypoxia-resistant nervous system of Aplysia – Hypoxia and ischemia tolerant species have been very useful for understanding the mechanisms by which such animals avoid the catastrophic nervous system damage encountered by humans subject to stroke or similar injuries. Aplysia are very resistant to hypoxia such that their simple, well studied nervous system should be an ideal model for investigating transcriptomic and metabolic adaptations that make this resistance possible. This would create a novel model for study of mitigation of hypoxic damage in the nervous system.

迈阿密大学的国家资源（资源）的使命是生产和 为生物医学研究提供始终如一的高质量养殖海兔， 通过应用研究提高该模型系统的实用性。海兔发挥了重要作用 在神经系统中细胞和分子变化如何构成基础的基础发现中的作用 行为，学习和记忆，由于其简单的神经系统的独特特征， 这类研究的理想模型系统。拟议继续实施这一资源方案的两个目标是： 1.生产和分配-资源将继续培养A。加州和使所有的生活 阶段，从卵到大型成虫，以合理的成本提供给研究人员。我们预计， 保持在每年10，000 - 15，000头动物的水平。我们也会满足用户的要求， 单独饲养、同龄组群、专门饮食或收获用于RNA或DNA的特定组织。在 此外，我们将提供成熟和衰老阶段的动物，使用由 资源，以及在我们计划的应用研究计划中开发的其他专门动物。 2.支持资源的应用研究计划-我们将进行两项应用研究 项目，以创造新的亚洲资源，我们相信这将扩大这个模型系统的有用性， 研究人员： 2.1衰老停滞的热量限制模型的分子表征-我们将 研究通过热量限制（CR）延长一年生命周期的机制。 我们将记录生命周期的各个部分，如生殖前、成熟和衰老， CR的。我们还将研究以前发现的与衰老相关的基因表达谱的改变 在这些阶段，并确定它们是如何受到CR的影响。该项目将扩大这些 动物作为衰老的模型。 2.2缺氧缺血性神经系统的分子特征-缺氧和 缺血耐受物种对于理解这些动物 避免遭受中风或类似损伤的人所遭遇的灾难性神经系统损伤。 失智症是非常耐缺氧，使他们的简单，充分研究的神经系统应该是一个理想的 研究转录组学和代谢适应的模型，使这种抗性成为可能。这将 建立了一种新的模型，用于研究减轻神经系统缺氧损伤。