Nanoassay development

荧光光谱本质上是一种非常敏感的技术；它已经形成了 这是大多数非放射性实时检测的基础，如聚合酶链式反应。我们的实验室之前曾与 以前的研究员(现在是生物技术行业的人)开发像“CataCave”这样的PCR替代品 SNPs的探测。 我们继续研究光物理和dna组件与载体(如多层金属纳米颗粒)的适当耦合。 对于更快的聚合酶链式反应分析和使用FCS(见MPM报告)来量化非常紧密 蛋白质-蛋白质和蛋白质-DNA在亚微升液滴中的结合(分析物 以亚毫微摩尔量存在)。我们目前正在设计用于DNA/RNA探测的STAQ探针(见纳米技术项目)，类似于瀑布，计划只超分辨紧密结合部位。 最近，我们已经使用TCSPC开始解决设计的适体异质性问题(参见MPM项目和TR项目)。适配子可以将其开启的荧光与分析物检测相结合，因此我们已经开始为RNA开发各种FRET传感概念。 我们还继续研究了与当地工业界合作以及最近在适配子合作中，除非展开或切割自猝灭的多肽和DNA“激子”探针的特征；激子耦合在新生的量子光学(特别是通信)中具有价值。菲尔德。 不幸的是，由于现场人员有限，合作努力受到了限制。

Fluorescence Spectroscopy is inherently a very sensitive technique; it already forms the basis of most non-radioactive real time assays like PCR. Our lab previously collaborated with former fellows (now in biotech industry) to develop alternatives to PCR like "CataCleave" probes for SNPs. We continue to study the photophysics and proper coupling of DNA components to carriers such as multilayer metal nanoparticles for much faster PCR analysis and the use of FCS (see MPM report) to quantify very tight protein-protein and protein-DNA binding in sub-microliter drops (analytes are present in sub-femtomole amounts). We are presently designing STAQ probes (see nanoscopy project) for DNA/RNA probing, analagous to catacleave, in plans to only superresolve tight binding sites. Recently we have used TCSPC to begin untangling designed aptamer heterogeneity questions (see MPM project and TR project). Aptamers can combine their turn-on fluorescence with analyte detection, so we have begun developing various FRET sensing concepts for RNA. We also continued to study the signatures for peptide and DNA "Excitonic" probes that self-quench unless unfolded or cleaved, in collaboration with local industry and most recently in aptamer collaborations ; excitonic coupling has value in the nascent quantum optics (communication esp.) field. Collaborative efforts have been unfortunately inhibited by limited on-site presence.