METABOLISM OF SOYBEAN ISOFLAVONOIDS AND LIGNANS IN MAN

大豆被发现含有异黄酮和木脂素。这些 二酚化合物已在许多体外细胞实验中得到证实 培养和动物实验显示具有抗增殖作用 与许多类型的癌症有关的特性，并已被证明 刺激许多白血病细胞系的分化。此外他们 具有许多其他生物效应，使它们成为强有力的候选者 作为癌症保护剂的作用。本次调查的目的是 继续开发定量测量方法 人体生物体液和食品样本中的木酚素和异黄酮类化合物 使用免疫学和 GC-MS 技术。多克隆和单克隆 将生产金雀异黄酮、大豆黄酮、雌马酚和 肠内酯。主要目标是简化技术 可以研究更大的人群。当这些方法准备好后 旨在测量已收集的血浆样本中的这些化合物 消费不同类型习惯饮食的年轻和老年妇女群体 （乳素食者、杂食者）和两组已接受手术的女性 用于早期乳腺癌。这些样品是独一无二的，因为我们拥有完整的 饮食记录和尿液中异黄酮和木酚素的测定结果 以及大量其他类固醇和肽激素 血浆、尿液和粪便（超过 50 种成分）。此外，它是 旨在继续研究抗增殖作用和 植物雌激素的作用机制，包括哺乳动物木脂素和 它们的前体是我们在恶性细胞上合成的 使用现代技术的线条。我们打算特别研究人类 乳腺癌、前列腺癌、子宫内膜癌和肝癌细胞。这些研究将 可以通过将化合物单独添加到细胞培养物中或添加到细胞培养物中来进行 组合或与各种雌激素、睾酮组合， 抗雌激素、与 II 型雌激素结合位点结合的化合物和 抗雌激素结合位点，全部已合成。 同时我们测量各种蛋白质，如前列腺特异性抗原 或培养基中的性激素结合球蛋白并使用分子生物学 阐明机制的技术。 我们还将继续合成参考化合物和氘- 用于 GC-MS 工作的标记化合物以及用于时间分辨的标记 免疫分析和放射免疫分析。该工作涉及抗原的合成 用于生产多克隆和单克隆抗体。该实验室位于 同时与化学系密切合作 大学自15年来提供了大量的氘代和 向研究小组提供木酚素和异黄酮类化合物的非氘化标准品 许多国家，主要是美国的研究小组。如果 我们可以获得持续的赠款支持。

Soybeans have been found to contain both isoflavonoids and lignans. These diphenolic compounds have in numerous experiments in vitro with cell cultures and in animal experiments been shown to have antiproliferative properties with regard to many types of cancer and have been shown to stimulate differentiation of many leukemic cell lines. In addition they have many other biological effects making them strong candidates for a role as cancer protective agents. The aim of the present investigation is to continue to develop methodology for quantitative measurements of lignans and isoflavonoids in human biological fluids and food specimens using immunological and GC-MS techniques. Polyclonal and monoclonal antibodies will be produced for genistein, daidzein, equol and enterolactone. The main goal is to simplify the techniques making it possible to study larger populations. When these methods are ready it is intended to measure these compounds in already collected plasma samples of groups of young and old women consuming different types of habitual diets (lactovegetarians, omnivores) and two groups of women having been operated for early breast cancer. These samples are unique because we have complete dietary records and assay results for isoflavonoids and lignans in urine as well as for a great number of other steroid and peptide hormones in plasma, urine and feces (more than 50 components). Furthermore it is intended to continue studies on the antiproliferative effects and mechanism of action of phytoestrogens including mammalian lignans and their precursors, which have been synthesized by us, on malignant cell lines using modern techniques. We intend to study particularly human breast, prostate, endometrium and liver cancer cells. These studies will be carried out by adding the compounds alone to cell cultures or in combination or combined with various estrogens, testosterone, antiestrogens, compounds binding to the type II estrogen binding site and antiestrogen binding site, which all have been synthesized already. Simultaneously we measure various proteins like prostatic specific antigen or sex hormone binding globulin in the medium and use molecular biology techniques for clarifying mechanisms. We will also continue the synthesis of reference compounds and deuterium- labelled compounds for GC-MS work as well as labels for time-resolved immunoassay and radioimmunoassay. The work involves synthesis of antigens for production of polyclonal and monclonal antibodies. This laboratory in close collaboration with the Department of Chemistry at the same university since 15 years has provided numerous deuterated and nondeuterated standards of lignans and isoflavonoids to research groups in many countries, mainly to research groups in USA. This we will continue if we can get continuation of the grant support.