METABOLISM OF SOYBEAN ISOFLAVONOIDS AND LIGNANS IN MAN

大豆被发现同时含有异黄酮类和木脂素。这些 二酚类化合物在体外与细胞进行了多次实验 培养和动物实验表明具有抗增殖剂 与许多类型的癌症有关的特性，并已被证明 刺激多种白血病细胞系分化。此外，他们还 具有许多其他生物效应，使它们成为 作为癌症保护剂的作用。目前调查的目的是 继续开发定量测量的方法 人体体液和食品样品中的木脂素和异黄酮类化合物 使用免疫学和GC-MS技术。多克隆和单克隆 将产生染料木素、大豆苷元、马酚和 肠内酯。主要的目标是简化制作它的技术 有可能研究更大的人口。当这些方法准备就绪时，它就是 旨在测量已经收集的血浆样本中的这些化合物 消费不同类型习惯性饮食的年轻和老年妇女群体 (乳素、杂食者)和两组接受手术的妇女 治疗早期乳腺癌。这些样品是独一无二的，因为我们有完整的 尿中异黄酮类和木脂素的膳食记录和检测结果 以及大量其他类固醇和多肽荷尔蒙 血浆、尿液和粪便(超过50种成分)。此外，它是 打算继续研究其抗增殖作用和 包括哺乳动物木脂素和植物雌激素的作用机制 它们的前体是由我们合成的，作用于恶性细胞 使用现代技术的线条。我们打算研究特别是人类 乳腺、前列腺、子宫内膜和肝癌细胞。这些研究将 通过将化合物单独添加到细胞培养物中或在 结合或结合各种雌激素，睾酮， 抗雌激素，与II型雌激素结合部位结合的化合物和 抗雌激素结合部位，这些都已经合成了。 同时，我们检测各种蛋白质，如前列腺特异性抗原 或性激素结合球蛋白的介质中，并使用分子生物学 阐明机制的技术。 我们还将继续合成参比化合物和氚- 用于GC-MS的标记化合物以及用于时间分辨的标记 免疫分析和放射免疫分析。这项工作涉及到抗原的合成 用于生产多克隆和单克隆抗体。这个实验室在 与化学系密切合作，同时 大学自15年以来提供了无数的氚和 年研究小组的木脂素和异黄酮类化合物的非氚标准 许多国家，主要是给美国的研究小组。如果出现以下情况，我们将继续这样做 我们可以获得持续的赠款支持。

Soybeans have been found to contain both isoflavonoids and lignans. These diphenolic compounds have in numerous experiments in vitro with cell cultures and in animal experiments been shown to have antiproliferative properties with regard to many types of cancer and have been shown to stimulate differentiation of many leukemic cell lines. In addition they have many other biological effects making them strong candidates for a role as cancer protective agents. The aim of the present investigation is to continue to develop methodology for quantitative measurements of lignans and isoflavonoids in human biological fluids and food specimens using immunological and GC-MS techniques. Polyclonal and monoclonal antibodies will be produced for genistein, daidzein, equol and enterolactone. The main goal is to simplify the techniques making it possible to study larger populations. When these methods are ready it is intended to measure these compounds in already collected plasma samples of groups of young and old women consuming different types of habitual diets (lactovegetarians, omnivores) and two groups of women having been operated for early breast cancer. These samples are unique because we have complete dietary records and assay results for isoflavonoids and lignans in urine as well as for a great number of other steroid and peptide hormones in plasma, urine and feces (more than 50 components). Furthermore it is intended to continue studies on the antiproliferative effects and mechanism of action of phytoestrogens including mammalian lignans and their precursors, which have been synthesized by us, on malignant cell lines using modern techniques. We intend to study particularly human breast, prostate, endometrium and liver cancer cells. These studies will be carried out by adding the compounds alone to cell cultures or in combination or combined with various estrogens, testosterone, antiestrogens, compounds binding to the type II estrogen binding site and antiestrogen binding site, which all have been synthesized already. Simultaneously we measure various proteins like prostatic specific antigen or sex hormone binding globulin in the medium and use molecular biology techniques for clarifying mechanisms. We will also continue the synthesis of reference compounds and deuterium- labelled compounds for GC-MS work as well as labels for time-resolved immunoassay and radioimmunoassay. The work involves synthesis of antigens for production of polyclonal and monclonal antibodies. This laboratory in close collaboration with the Department of Chemistry at the same university since 15 years has provided numerous deuterated and nondeuterated standards of lignans and isoflavonoids to research groups in many countries, mainly to research groups in USA. This we will continue if we can get continuation of the grant support.