VITAMIN B12 PHYSIOLOGY AND METABOLISM

描述：研究者将确定所有基因的功能， 允许丙二醇/连四硫酸盐依赖性厌氧 增长 这将涉及完成pdu操纵子的DNA序列， 参与转化的pdu基因的表征 氰钴胺到维生素B12-钴胺素，一种独特的细胞器的特征 (CLOs沙门氏菌的羧基体样细胞器），并鉴定 催化丙二醇分解的基因和测定 PDU基因是否参与钴胺素交换。 他们还将扩大 他们对丙二醇发酵和厌氧呼吸的研究 和乙醇胺，并且，给定时间，它们将确定 推测的新的可立奈参与连四硫酸盐还原。

DESCRIPTION: The investigator will determine the functions of all genes in the pdu operon that allows propanediol/tetrathionate-dependent anaerobic growth. This will involve completion of the DNA sequence of the pdu operon, a characterization of the pdu genes involved in the conversion of cyanocobalamin to Ado-cobalamin, a characterization of the unique organelle (CLOs carboxysome-like organelles of Salmonella), and identification of the genes that catalyze the catabolism of propanediol and determination of whether pdu genes are involved in cobalamin exchange. They will also extend their studies on the fermentation and anaerobic respiration of propanediol and ethanolamine and, given time, they will determine the structure of a putative novel corrinide involved in tetrathionate reduction.