MECHANISMS OF RECURRENCE OF ANTERIOR UVEITIS OF THE EYE

眼睛前葡萄膜炎的复发机制

• 批准号: 2444266
• 负责人: Thomas Frank Freddo
• 金额: $ 33.39万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-01-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2444266
• 关键词: X ray; blood aqueous barrier; cellular pathology; clinical research; confocal scanning microscopy; electron microscopy; fluorescent dye /probe; fluorimetry; freeze etching; human subject; inflammation; iridocyclitis; iris; laboratory rabbit; magnetic resonance imaging; mathematical model; membrane permeability; molecular pathology; morphology; pathologic process; relapse /recurrence; tight junctions; uvea ciliary body; vascular endothelium

DESCRIPTION (Adapted from applicant's abstract): This proposal is a multidisciplinary approach to examine the hypothesis that the initial AU alters the BAB permeability, setting up stages for recurrence. The basis of this application will be to evaluate the tight junctions of the BAB following an intravitreal injection of endotoxin. The applicant will then look at the BAB repair in both the ciliary epithelium and iris vascular endothelium at several levels. The applicant has past experience with these techniques, including in vivo aqueous fluorophotometry and tracer localization with EM, computational modeling of the protein kinetics and confocal and electron microscopy localization of molecular probes relevant to the junctional reassembly. Freeze fracture will also be conducted. The last specific aim will be to use high resolution contract MRI to determine if anterior diffusion pathways exists in normal humans.

描述(改编自申请人的摘要)：本建议书是 用多学科方法检验初始AU的假设 改变Bab的渗透性，为复发奠定基础。的基础 这个应用程序将评估BAB的紧密连接 在玻璃体内注射了内毒素之后。然后，申请者将 观察睫状体上皮和虹膜血管的Bab修复 不同水平的内皮细胞。申请者过去有过这些经验 技术，包括活体水相荧光光度和示踪剂 EM定位，蛋白质动力学的计算模型和 分子探针的共聚焦和电子显微镜定位 结合部的重组。还将进行冷冻骨折。这个 最后一个具体目标是使用高分辨率合同MRI来确定 如果在正常人类中存在前扩散路径。