MECHANISMS OF RECURRENCE OF ANTERIOR UVEITIS OF THE EYE

男性特发性前葡萄膜炎的特点是 血-房水屏障(BAB)是一种急性且经常复发的疾病。左边 如果不治疗，它可能会导致继发性青光眼或其他并发症。 目前可用的严重程度的客观衡量标准提供的 关于炎症过程动态的信息， 尤其是解析过程中的BAB修复过程。了解这些知识 这一过程取决于对蛋白质扩散的透彻理解 正常和发炎的眼睛里的机械师。 我们在这些动物研究中的目标是提炼和验证准确的 用于为BAB获取更多信息的计算模型 非侵入性目标法对炎症眼的通透性研究 水相荧光分光光度法(AF)。使用荧光辣根 过氧化物酶，一种由我们小组首次推出的血管内示踪剂，我们有 成功地将体内房颤研究的结果与 显微示踪器定位研究，都是在同一只眼睛上进行的， 利用这种组合的AFL方法，可以区分哪些位置 有，从那些没有贡献的水中的荧光 眼睛发炎，由AF测量；这是一个有价值的工具，可以用来证实 计算模型的某些预测。 使用我们的AFL方法，我们将检查重建 前葡萄膜炎消融术中BAB的应用 使用类固醇和非类固醇抗炎药治疗。 将特别强调以下机制 粘连带和咬合带的重组(紧密 结点)，它们构成了BAB的解剖学等价物。所有的 上述程序可与冻结- 断口，电子显微镜和共聚焦，激光扫描显微镜 研究某些蛋白质在连接重组中的作用， 如F-肌动蛋白、E-9和ZO-1。BAB修复不完整或有故障 渗透性，在临床证据表明急性前葡萄膜炎有 消退，可以为免疫复合体的沉积提供储存库， 可能是导致复发性前葡萄膜炎的原因。我们还将检查 被炎症破坏的缝隙连接重新出现在 纤毛上皮。这些结合点在经济复苏中的贡献作用 从炎症引起的眼压下降将被寻求。

Idiopathic anterior uveitis in man is characterized by a breakdown of the blood-aqueous barrier (BAB) that is acute and often recurrent. Left untreated, it can lead to secondary glaucoma, or other complications. Currently available objective measures of severity provide little information regarding the dynamics of the inflammatory processes, especially the process of BAB repair during resolution. Knowledge of these process depends upon a thorough understanding of protein diffusional mechanics in the normal and inflamed eye. Our goal in these animals studies is to refine and validate an accurate computational model for use in deriving more information for BAB permeability studies of inflamed eyes using the non-invasive objective method of aqueous fluorophotometry (AF). Using fluoresceinated horseradish peroxidase, an intravascular tracer first introduced by our group, we have successfully compared results of in vivo AF studies with results of microscopic Tracer Localization studies, both perform on the same eyes, With this combined AFL method, it is possible to distinguish sites which have, from those which have not contributed to aqueous fluorescence in the inflamed eye, as measured by AF; a valuable tool with which corroborate certain predictions of computational models. Using our AFL method, we will examine the process of re-establishment of the BAB during resolution of anterior uveitis, both with and without treatment using steroidal and non-steroidal anti-inflammatory agents. Particular emphasis will be given to the mechanisms involved in the reassembly of zonulae adhaerentes and zonulae occludentes (tight junctions), which constitute the anatomic equivalents of the BAB. All of the procedures outlined above can be performed in conjunction with freeze- fracture, election microscopy and confocal,laser scanning microscopy to examine the roles of certain proteins involved in junctional re-assembly, such as F-actin, E-9 and ZO-1. Incomplete or faulty restoration of BAB permeability, after clinical evidence of an acute anterior uveitis has subsided, could provide repositories for immune-complex deposition, possibly contributing to recurrent anterior uveitis. We will also examine the re-appearance of gap junctions which are destroy by inflammation in the ciliary epithelium. A contributing role for these junctions in recovery from inflammation-induced ocular hypotension will be sought.