Targeting of LOS for Treatment of Antibiotic-Resistant Neisseria gonorrhoeae

LOS 靶向治疗抗生素耐药性淋病奈瑟菌

• 批准号: 10617635
• 负责人: Gary A Jarvis
• 金额: --
• 依托单位: VETERANS AFFAIRS MED CTR SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10617635
• 关键词: Amino Acids; Anabolism; Anti-Bacterial Agents; Antibiotic Therapy; Antibiotics; Antimicrobial Cationic Peptides; Azithromycin; Bacteria; Binding Proteins; Biological Assay; Canada; Cardiovascular system; Cefixime; Ceftriaxone; Cells; Cephalosporins; Cervical; Classification; Clinical; Complement; Country; Cytolysis; DNA Binding; Data; Denmark; Drug Kinetics; Drug-resistant Neisseria Gonorrhoeae; Ectopic Pregnancy; Environment; Enzymes; Epithelial Cells; Evaluation; Exposure to; Female; Generations; Gonorrhea; Gram-Negative Bacteria; HIV; Healthcare; Healthcare Systems; Hemolysis; Human; Immune response; In Vitro; Incidence; Infection; Infertility; Inflammation; Inflammatory; Innate Immune System; Invaded; Investigation; Japan; Lipid A; Measurable; Mediating; Membrane; Military Personnel; Mission; Modeling; Morbidity - disease rate; Multi-Drug Resistance; Neisseria gonorrhoeae; Outcome; Patient Care; Pelvic Inflammatory Disease; Penetration; Peptides; Permeability; Predisposition; Proteolysis; Public Health; Recommendation; Reporting; Research; Resistance; Resistance development; Risk Behaviors; Sexually Transmitted Diseases; Testing; Therapeutic; Time; Toxic effect; Vaccines; Variant; Veterans; Woman; Work; active duty; antimicrobial; bactericide; chronic pelvic pain; cytokine; cytotoxicity; design; efficacy testing; efflux pump; high risk; in vitro Assay; in vitro testing; in vivo; in vivo evaluation; inhibitor; interest; lead candidate; lipid biosynthesis; lipooligosaccharide; men; military service; mouse model; neutrophil; novel; novel therapeutics; prevent; reproductive tract; resistant strain; transmission process

Infections due to N. gonorrhoeae are a major cause of morbidity with an estimated 850,000 cases in the U.S. and 87 million cases worldwide annually. Within the VA Health Care System, cases of gonorrhea increased between 2013 and 2017 with the total number in that time period at 10,587. The most serious sequelae are suffered by infected women as gonococci ascend to the upper reproductive tract and cause pelvic inflammatory disease in 10-20% of women with infections, which encompasses a wide range of inflammatory conditions and often leads to chronic pelvic pain, infertility, and ectopic pregnancy. There is no vaccine to N. gonorrhoeae and a great need for new antibiotics due to the alarming rise in multidrug-resistance (MDR), which is making emergence of untreatable gonococcal infections a real prospect. Currently only ceftriaxone and azithromycin are recommended for first-line therapy, and clinical isolates resistant to both of those antibiotics have been reported in countries including Denmark, Canada, and Japan. Thus, there is a compelling need for new antimicrobials for gonococcal infections. Our studies to date of N. gonorrhoeae lipooligosaccharide (LOS) and the human innate immune system have shown that the lipid A portion of LOS is the primary inducer of cytokine-mediated inflammation and investigations by others have shown that the lipid A also facilitates gonococcal infection. These data led us to the concept that targeting lipid A biosynthesis would be an effective approach to combating N. gonorrhoeae infections. We recently reported that inhibition of LpxC, the enzyme that catalyzes the second step of lipid A biosynthesis, was bactericidal for nine multidrug-resistant and human challenge strains of gonococci and reduced cytokine induction without apparent human cell cytotoxicity. From the LpxC inhibitor data, we postulated that membrane disruption due to the inhibition of LOS biosynthesis was lethal for gonococci. To investigate this, we recently evaluated the bactericidal potential of a 12 amino acid cell-penetrating peptide (CPP) for MDR and human challenge strains of N. gonorrhoeae and found that the CPP penetrated the bacterial membrane and was bactericidal for all nine MDR and human challenge strains of gonococci tested. Importantly, no apparent resistance to the CPP developed in surviving bacteria as susceptibility was the same in bacteria from colonies after exposure to CPP and then retreated. Further, the CPP reduced inflammatory cytokine induction and prevented bacterial cell invasion of cervical epithelial cells in the absence of measurable cell cytotoxicity. These novel data highlight LpxC and CPP as promising antimicrobials for N. gonorrhoeae and strongly support the hypothesis of this application that inhibiting the biosynthesis of lipid A components with LpxC inhibitors and disrupting outer membrane integrity with CPP will impact bacterial viability and host response to N. gonorrhoeae infection in vitro and in vivo, which will have a therapeutic impact on infection outcomes. This project is focused on optimizing and testing the efficacy of the CPP and LpxC inhibitor in relevant in vitro assays of bactericidal activity, cytokine induction, hemolysis, and cell cytotoxicity. Mechanistic studies will include investigations of DNA binding, cell permeabilization, proteolysis resistance, protein binding and the effect of the MtrCDE, MacAB and NorM gonococcal efflux pumps. The lead candidate CPP and LpxC inhibitor identified in vitro will be tested for in vivo efficacy, pharmacokinetics and cardiovascular toxicity in an established female mouse model of gonococcal genital tract infection that has been increasingly used for evaluation of candidate antimicrobials for treatment of gonorrhea. We expect that the results from our studies will demonstrate the efficacy of these two antimicrobials as new therapeutics for N. gonorrhoeae infection, which are urgently needed given the rise in MDR gonococcal strains. This will be the first study of its kind to test these two classes of antimicrobials for efficacy against gonorrhea.

由淋病奈瑟菌引起的感染是发病率的主要原因，在美国估计有85万例

项目成果

Novel small molecules that increase the susceptibility of Neisseria gonorrhoeae to cationic antimicrobial peptides by inhibiting lipid A phosphoethanolamine transferase.

• DOI: 10.1093/jac/dkac204
• 发表时间: 2022-08-25
• 期刊: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
• 影响因子: 5.2
• 作者: Mullally, Christopher;Stubbs, Keith A.;Thai, Van C.;Anandan, Anandhi;Bartley, Stephanie;Scanlon, Martin J.;Jarvis, Gary A.;John, Constance M.;Lim, Katherine Y. L.;Sullivan, Courtney M.;Sarkar-Tyson, Mitali;Vrielink, Alice;Kahler, Charlene M.
• 通讯作者: Kahler, Charlene M.