Core 3: Chemical Biology & Materials Tools (CBMT)

项目摘要 用于探测、操纵和成像生物系统的化学生物学工具可以提供 对基本流程的深入了解。化学二聚化与蛋白质定位 用于分析物检测的策略、荧光探针和传感器，以及用于 扰乱生物途径和过程已成为收集信息的重要研究工具 对生物过程有更深入的了解。这些光触发变体的发展 重要的化学生物学工具为探测提供了新的和更强大的方法， 利用新的空间和时间层次操纵和成像关键路径和过程 控件以前不可用。尽管分子化学生物学方法很有用， 由于需要按需合成，许多工具和试剂并不容易获得 和分子定制取决于正在研究的路径和过程以及 缺乏商业上的可用性。拟议的化学生物学和材料核心将解决 这些问题是通过直接与个别项目合作的方案来设计的， 为项目定制合成和实施尖端的化学生物工具 研究目的。化学生物学核心也将作为整个奖项的一个资源 与MAC核心协同开发新方法和实施关键技术的时期 以及个别项目。在目标1中，核心将开发用于光遗传控制的化学二聚体 关键的生物分子定位和激活事件。在目标2中，核心将开发基于 用于询问关键生物途径和过程的化学探针。

Project Summary Chemical Biology tools for probing, manipulating, and imaging biological systems can provide deep insights into fundamental processes. Chemical dimerization and protein localization strategies, fluorescent probes and sensors for analyte detection, and small molecules for perturbing biological pathways and process have become important research tools for gleaning deeper insight into biological processes. The development of optically triggered variants of these important chemical biology tools has provided new and more powerful approaches to probing, manipulating, and imaging key pathways and processes with new levels of spatial and temporal control not previously available. Despite the utility of molecular chemical biology approaches, many of the tools and reagents are not readily available due to the need for on-demand synthesis and molecular customization depending on the pathways and processes being studied and the lack of commercially availability. The proposed Chemical Biology and Materials Core will solve these problems by working directly with the individual projects of the program to design, synthesize, and implement cutting edge Chemical biology tools customized to the project research aims. The Chemical Biology Core will also serve as a resource throughout the award period to develop new approaches and implement key technologies in synergy with the MAC core and individual projects. In Aim 1 the core will develop chemical dimerizers for optogenetic control of key biomolecule localization and activation events. In Aim 2 the core will develop peptide based chemical probes for interrogation of key biological pathways and processes.