AMINO ACID ANALYSIS USING MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

生物成分的痕量分析已成为关键 朝向现代生物化学和医学的发展。一 特别强大的贡献是发展 能够快速、定量地进行分离的技术 对少量生物质的分析。高性能液体 层析(高效液相)已被证明能够自然分解所有 在相对较短的时间范围内出现的氨基酸提供了强大的 蛋白质和蛋白质氨基酸组成的测定方法 多肽。高效液相色谱的一个重大缺点以及其他许多缺点 目前可用的分析方法，是生物量 执行分析所需的样本，因为英勇的努力通常 需要分离出感兴趣的成分(S)。作为一个概念，更多 正在开发灵敏的微量分析分离方案，以提供 更高的质量灵敏度和更高的分辨率。其中 这些新技术，毛细管电泳法(CE)已被证明是一种 性能出众、成本效益高的工具。 该项目的总体目标是扩展 胶束电动毛细管色谱(MECC)，CE的一种模式， 向生物材料的微量分析迈进。建议进行的研究 将评估一系列用于促进变化的新型有机添加剂 在电色谱对生物来源分子的选择性方面 低浓度的胶束体系。更具体地说，目标是 提供一种快速且可重复的分离方案 用Femto完全解析所有自然产生的氨基酸- 摩尔质量灵敏度。这一目标将通过评估来实现 一系列新型有机添加剂与传统胶束的结合 确定它们对电色谱保留值和 通过一系列系统的研究来选择性地改变每个 层析参数独立。基于对……的坚定理解 这些添加剂在胶束体系中的行为，优化了 MECC法测定衍生氨基酸的含量 将会得到解决。这个项目将提供更好的了解 MECC中修饰胶束相对生物来源物种的作用 如果成功，将为微量分析提供一种经济高效的替代方案 氨基酸的含量。

The trace analysis of biological components has been a critical key towards the development of modern biochemistry and medicine. One particularly powerful contribution has been the development of separations techniques capable of performing rapid and quantitative analysis on small amounts of biogenic materials. High performance liquid chromatography (HPLC) has proven capable of resolving all naturally occurring amino acids in a relatively short time frame providing a robust method for the determination of amino acid composition of proteins and peptides. One significant drawback to HPLC, as well as many other currently available analysis methods, is the quantity of biological sample required to perform the analysis since heroic efforts are often required to isolate the components(s) of interest. As a conseuence, more sensitive microanalysis separation schemes are being developed to provide increased mass sensitivities and improved resolving capabilities. Among these new techniques, capillary electrophoresis (CE) has proved to be an exceptionally capable and cost effective tool. The overall objective of this project is to extend the utility of micellar electrokinetic capillary chromatography (MECC), a mode of CE, towards the microanalysis of biological materials. The proposed studies will evaluate a novel series of organic additives used to promote changes in the electrochromatographic selectivity towards biogenic molecules in micellar systems at low concentrations. More specifically, the aim is to provide a rapid and reproducible separation scheme that produces complete resolution of all naturally occurring amino-acids with femto- mole mass sensitivities. This objective will be approached by evaluating a novel series of organic additives combined with traditional micellar phases to determine their effects on electrochromatographic retention and selectivity through a series of systematic studies altering each chromatographic parameter independently. Upon a firm understanding of the behavior of these additives in micellar systems, optimization of a MECC method for the quantitative determination of derivatized amino acids will be addressed. This project will provide a better understanding of the utility of modified micellar phases in MECC towards biogenic species and if successful provide a cost effective alternative to microanalysis of amino acids.