ANALYSES OF A NEW TETRAHYMENA CILIARY DYNEIN
ANALYSES OF A NEW TETRAHYMENA CILIARY DYNEIN
批准号:
2193658
负责人:
DAVID G PENNOCK
金额:
$10.3万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-06-01 至 1999-07-31
中文摘要
纤毛和鞭毛是复杂的微管细胞器,普遍存在
英文摘要
Cilia and flagella are complex microtubular organelles that are ubiquitous
among eukaryotes, including humans, where they are found in the
respiratory tract, the female reproductive tract and on sperm cells.
Ciliary and flagellar dysfunction resulting from the absence of dynein
arms has been reported to cause respiratory and fertility problems in
humans and other mammals. The long term objectives of this work are to
understand the mechanisms by which dyneins assemble into ciliary axonemes
and to understand the role(s) of dyneins in ciliary motility by using the
unicellular protozoan, Tetrahymena thermophila. as a model system.
Specifically, the role(s) that a newly discovered ciliary dynein heavy
chain (sky chain dynein heavy chain) plays in ciliary motility will be
determined by a) cloning, sequencing and analyzing the gene encoding the
sky chain dynein heavy chain, b) introducing mutations into the sky chain
dynein heavy chain gene using targeted gene knock out techniques, and c)
analyzing the mutant strains to allow inferences to be drawn about the
location and function of the sky chain dynein heavy chain. To clone the
sky chain gene, the sky chain will be isolated and partially sequenced.
The sequences will be used to design oligonucleotide primers. The primers
will be used in the polymerase chain reaction to amplify DNA encoding a
portion of the sky chain dynein heavy chain gene. To create the knockout
vector, an expression cassette containing a selectable marker will be
inserted into the middle of the sky chain gene. The knock out vector will
be transformed into conjugating Tetrahymena under conditions that promote
homologous recombination between the transforming DNA and the target
sequence. This should yield mutant strains in which endogenous sky chain
genes are inactivated by insertion of the expression cassette and thus,
are missing the sky chain dynein heavy chain. Mutant strains will be
subjected to biochemical and ultrastructural analyses to determine the
location and function of the newly discovered sky chain dynein heavy
chain. The results of this work should add to our understanding of dynein
organization and function in cilia and flagella.
期刊论文(3)
专著(0)
科研奖励(0)
会议论文
Selection of motility mutants.
选择运动突变体。
DOI:
10.1016/s0091-679x(08)61537-x
发表时间:
2000
期刊:
Methods in cell biology
影响因子:
--
作者:
[Pennock,DG]
通讯作者:
Pennock,DG
The dynein heavy chain gene family in Tetrahymena thermophila.
嗜热四膜虫的动力蛋白重链基因家族。
DOI:
10.1111/j.1550-7408.1999.tb05136.x
发表时间:
1999
期刊:
The Journal of eukaryotic microbiology
影响因子:
--
作者:
[Xu,W, Royalty,MP, Zimmerman,JR, Angus,SP, Pennock,DG]
通讯作者:
Pennock,DG
New axonemal dynein heavy chains from Tetrahymena thermophila.
来自嗜热四膜虫的新轴丝动力蛋白重链。
DOI:
10.1111/j.1550-7408.1999.tb04598.x
发表时间:
1999
期刊:
The Journal of eukaryotic microbiology
影响因子:
--
作者:
[Mobberley,PS, Sullivan,JL, Angus,SP, Kong,X, Pennock,DG]
通讯作者:
Pennock,DG
ANALYSES OF DYNEIN HEAVY CHAIN MUTATION IN TETRAHYMENA
-
批准号:2888680
-
项目类别:
-
资助金额:$10.32万
-
财政年份:1999
-
负责人:DAVID G PENNOCK
-
依托单位:
Function and Regulation of Inner Arm Dyneins
-
批准号:6953533
-
项目类别:
-
资助金额:$20.46万
-
财政年份:1999
-
负责人:DAVID G PENNOCK
-
依托单位:
Organization and function of inner arm dyneins
-
批准号:6456883
-
项目类别:
-
资助金额:$13.24万
-
财政年份:1999
-
负责人:DAVID G PENNOCK
-
依托单位:
ANALYSES OF TETRAHYMENA MUTANTS LACKING OUTER DYNEIN
-
批准号:2187024
-
项目类别:
-
资助金额:$9.97万
-
财政年份:1993
-
负责人:DAVID G PENNOCK
-
依托单位:
CONTROL OF CILIA BIOGENESIS IN TETRAHYMENA THERMOPHILA
-
批准号:3438701
-
项目类别:
-
资助金额:$10.35万
-
财政年份:1990
-
负责人:DAVID G PENNOCK
-
依托单位: