ELECTROGENERATED CHEMILUMINESCENCE SENSOR ASSEMBLIES

总体目标是开发和演示一种小型光纤 电致化学发光(CL)探头 用于测定溶液中的痕量浓度； 鲁米诺和Ru(Bipy)2+3CL体系都将被使用。 目标依次为：1)评估定位 在离光纤束较小距离处的工作电极 与将光学透明电极直接沉积在 束的表面。2)检查溶解CL试剂的使用情况与 将CL试剂掺入薄聚合物薄膜中 工作电极的表面。3)检查以下情况的后果 搅动解决方案，而不是让它静止不动。4) 确定工作范围、检测下限和精密度 过氧化氢和鲁米诺的测定(鲁米诺CL法) 草酸盐和Ru(Bipy)2+3(Ru(Bipy)2+3CL)的测定。 鲁米诺CL已证明适用于测定 可以转化为等量氢的物种 过氧化氢(如葡萄糖、肌酐、胆固醇等)为了. 在免疫分析中可以用鲁米诺标记的物种。 Ru(Bipy)2+3CL可用于测定可氧化物种，如 草酸盐和胺以及用Ru(Bipy)2+3标记的物种 衍生品。电致化学发光使人们可以避免样品 通过添加催化剂进行稀释，开启和关闭反应 通过控制电极电位和物理定位化学发光 工作电极表面的发射。 他们的计划是“对样品进行测量”。传感器 直径可以小于5毫米，因此可以在小范围内探测样本 小瓶。一种传感器方案是使用铂丝电极 位于距光纤束末端约1 mm处； 第二种方法是用气相沉积一层薄薄的 光学透明电极材料(氧化锡或金) 光纤束的抛光表面。 溶液pH、电极电位、化学发光试剂的影响 浓度，溶解与聚合物包埋的CL试剂和 我们将研究静置溶液与搅拌溶液的对比。以求确定 鲁米诺(或鲁米诺标记物种)双工作电极将 一种用于生成过氧化剂，另一种用于 电产生鲁米诺CL。

The overall aim is to develop and demonstrate a small fiber optic probe that incorporates electrogeneration of chemiluminescence (CL) to be used for determination of trace concentrations in solution; both the luminol and the Ru(bipy)2+3 CL systems will be utilized. Aims to be addressed in turn are: 1) Evaluate positioning the working electrode at a small distance from the fiber optic bundle vs. depositing an optically transparent electrode directly upon the surface of the bundle. 2) Examine use of dissolve CL reagent vs. incorporation of the CL reagent into a thin polymeric film on the surface of the working electrode. 3) Examine consequences of stirring the solution vs. allowing it to remain still. 4) Determine working ranges, detection limits, and precisions for determinations of hydrogen peroxide and luminol (with luminol CL) and determination of oxalate and Ru(bipy)2+3 (with Ru(bipy)2+3 CL). Luminol CL has demonstrated applicability for determination of species that can be converted to an equivalent amount of hydrogen peroxide (e.g. glucose, creatinine, cholesterol, etc.) and for species that can be tagged with luminol as in immunoassay. Ru(bipy)2+3 CL can be used to determine oxidixable species like oxalate and amines and also species tagged with Ru(bipy)2+3 derivatives. Electrogeneration of CL allows one to avoid sample dilution from addition of catalyst, turn the reaction on and off by control of electrode potential, and physically localize CL emission at the working electrode surface. The plan is to "take the measurement to the sample." The sensor could be under 5 mm diameter so could probe a sample in a small vial. One sensor plan would be to use a platinum wire electrode located approximately 1 mm from the end of an optical fiber bundle; a second approach would be to vapor deposit a thin layer of an optically transparent electrode material (tin oxide or gold) onto the polished surface of the fiber optic bundle. The effect of solution pH, electrode potential, CL reagent concentration, dissolved vs. polymer-entrapped CL reagents and still vs. stirred solution will be investigated. For determination of luminol (or luminol-tagged species) dual working electrodes will be used, one to generate the peroxide reagent and one to electrogenerate luminol CL.