ORGANIZATION OF THE AXON MEMBRANE

轴突膜的重组通常与病理有关。 伴随脱髓鞘疾病的情况，如多发性硬化症 硬化症。这项研究的重点是分子的组织和 轴突膜在发育、髓鞘形成和发育过程中的动态变化 组织培养模型中的脱髓鞘神经。 我们将研究可兴奋膜组分的分布。 大鼠有髓纤维的免疫荧光和电子显微镜观察 周围神经系统和那些已经实验过的纤维 脱髓鞘。因为我们对动态变化特别感兴趣 在髓鞘形成过程中发生在轴突膜的组织中，我们 将监测可兴奋性膜蛋白的顺序变化 单个神经元的数字荧光显微镜地形图研究 轴突形成时感觉神经元和雪旺细胞的长期培养 被套住了。细胞内和/或细胞外的作用和大小 在发育过程中组织轴突细胞膜的分子， 脱髓鞘的检查将通过测量 荧光光漂白法测定钠通道、糖蛋白和脂类 恢复。感觉神经元在缺乏或存在的情况下培养 雪旺细胞将被用于评估轴突-神经胶质接触的贡献 在发育过程中调节轴突细胞膜的组织。这个 包膜细胞在维持结节和节间中的作用 专业化认证将通过开发、脱髓鞘和 再生髓鞘纤维。 表达、集合和表达中的时间和空间事件 NaChs与细胞外基质和细胞骨架的分离将是 检查过了。具体地说，我们将继续研究两者粘连的作用 分子、锚蛋白和血影蛋白在建立限制性膜中的作用 可以用来维持可兴奋的膜组件的结构域 轴突的特定区域。

Reorganization of the axonal membrane is often associated with pathological conditions that accompany demyelinating disorders such as multiple sclerosis. This research program focuses on the molecular organization and dynamics of the axonal membrane in developing, myelinating, and demyelinated nerve in tissue culture models. We will examine the distribution of excitable membrane components by immune-fluorescence and electron microscopy in myelinated fibers in the peripheral nervous systems and those fibers that have been experimentally demyelinated. Because we are particularly interested in the dynamic changes occurring in the organization of the axon membrane during myelination, we will monitor the sequential changes in excitable membrane protein topography by digital fluorescence microscopy on single neurons in long-term cultures of sensory neurons and Schwann cells as the axons become ensheathed. The role and magnitude of intracellular and/or extracellular elements that organize the axon membrane during development, ensheathment, and demyelination will be examined by measuring the lateral mobility of sodium channels, glycoproteins, and lipids by fluorescence photobleach recovery. Cultures of sensory neurons either in the absence or presence of Schwann cells will be used to assess the contribution of axon-glial contact in modulating the organization of the axon membrane during development. The role of the ensheathing cell in maintaining nodal and internodal specializations will be examined with developing, demyelinated, and remyelinating fibers. The temporal and spatial events in the expression, assembly, and segregation of NaChs with the extracellular matrix and cytoskeleton will be examined. Specifically, we will continue to study the role of both adhesion molecules, ankyrin, and spectrin in establishing restricted membrane domains that could serve to maintain excitable membrane components to specific regions of the axon.