ORGANIZATION OF THE AXON MEMBRANE
ORGANIZATION OF THE AXON MEMBRANE
批准号:
3414533
负责人:
KIMON J ANGELIDES
金额:
$18.63万
依托单位国家:
美国
项目类别:
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-08-01 至 1995-07-31
关键词:
Schwann cells afferent nerve ankyrins axon cell adhesion cytoskeleton developmental neurobiology electron microscopy embryo /fetus tissue /cell culture extracellular matrix glia glycoproteins immunocytochemistry immunoelectron microscopy immunofluorescence technique lipids membrane proteins membrane structure myelination myelinopathy node of Ranvier peripheral nervous system sodium channel spectrin
中文摘要
轴突膜的重组通常与病理有关。
伴随脱髓鞘疾病的情况,如多发性硬化症
硬化症。这项研究的重点是分子的组织和
轴突膜在发育、髓鞘形成和发育过程中的动态变化
组织培养模型中的脱髓鞘神经。
我们将研究可兴奋膜组分的分布。
大鼠有髓纤维的免疫荧光和电子显微镜观察
周围神经系统和那些已经实验过的纤维
脱髓鞘。因为我们对动态变化特别感兴趣
在髓鞘形成过程中发生在轴突膜的组织中,我们
将监测可兴奋性膜蛋白的顺序变化
单个神经元的数字荧光显微镜地形图研究
轴突形成时感觉神经元和雪旺细胞的长期培养
被套住了。细胞内和/或细胞外的作用和大小
在发育过程中组织轴突细胞膜的分子,
脱髓鞘的检查将通过测量
荧光光漂白法测定钠通道、糖蛋白和脂类
恢复。感觉神经元在缺乏或存在的情况下培养
雪旺细胞将被用于评估轴突-神经胶质接触的贡献
在发育过程中调节轴突细胞膜的组织。这个
包膜细胞在维持结节和节间中的作用
专业化认证将通过开发、脱髓鞘和
再生髓鞘纤维。
表达、集合和表达中的时间和空间事件
NaChs与细胞外基质和细胞骨架的分离将是
检查过了。具体地说,我们将继续研究两者粘连的作用
分子、锚蛋白和血影蛋白在建立限制性膜中的作用
可以用来维持可兴奋的膜组件的结构域
轴突的特定区域。
英文摘要
Reorganization of the axonal membrane is often associated with pathological
conditions that accompany demyelinating disorders such as multiple
sclerosis. This research program focuses on the molecular organization and
dynamics of the axonal membrane in developing, myelinating, and
demyelinated nerve in tissue culture models.
We will examine the distribution of excitable membrane components by
immune-fluorescence and electron microscopy in myelinated fibers in the
peripheral nervous systems and those fibers that have been experimentally
demyelinated. Because we are particularly interested in the dynamic changes
occurring in the organization of the axon membrane during myelination, we
will monitor the sequential changes in excitable membrane protein
topography by digital fluorescence microscopy on single neurons in
long-term cultures of sensory neurons and Schwann cells as the axons become
ensheathed. The role and magnitude of intracellular and/or extracellular
elements that organize the axon membrane during development, ensheathment,
and demyelination will be examined by measuring the lateral mobility of
sodium channels, glycoproteins, and lipids by fluorescence photobleach
recovery. Cultures of sensory neurons either in the absence or presence of
Schwann cells will be used to assess the contribution of axon-glial contact
in modulating the organization of the axon membrane during development. The
role of the ensheathing cell in maintaining nodal and internodal
specializations will be examined with developing, demyelinated, and
remyelinating fibers.
The temporal and spatial events in the expression, assembly, and
segregation of NaChs with the extracellular matrix and cytoskeleton will be
examined. Specifically, we will continue to study the role of both adhesion
molecules, ankyrin, and spectrin in establishing restricted membrane
domains that could serve to maintain excitable membrane components to
specific regions of the axon.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
MOLECULAR DYNAMICS MULTIPROBE CONFOCAL LASER MICROSCOPE
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批准号:2284439
-
项目类别:
-
资助金额:$7.8万
-
财政年份:1994
-
负责人:KIMON J ANGELIDES
-
依托单位:
MOLECULAR CYTOLOGY OF INTERMEDIATE FILAMENTS IN NERVE
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批准号:2266068
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项目类别:
-
资助金额:$23.31万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
-
依托单位:
MOLECULAR CYTOLOGY OF INTERMEDIATE FILAMENTS IN NERVE
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批准号:3412707
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项目类别:
-
资助金额:$17.37万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
-
依托单位:
MOLECULAR CYTOLOGY OF INTERMEDIATE FILAMENTS IN NERVE
-
批准号:3412706
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项目类别:
-
资助金额:$16.45万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
-
依托单位:
MOLECULAR CYTOLOGY OF INTERMEDIATE FILAMENTS IN NERVE
-
批准号:3412708
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项目类别:
-
资助金额:$22.34万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
-
依托单位:
MOLECULAR CYTOLOGY OF INTERMEDIATE FILAMENTS IN NERVE
-
批准号:3412704
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项目类别:
-
资助金额:$15.52万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
-
依托单位:
ORGANIZATION OF THE AXON MEMBRANE
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批准号:2266746
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项目类别:
-
资助金额:$26.67万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
-
依托单位:
ORGANIZATION OF THE AXON MEMBRANE
-
批准号:3414535
-
项目类别:
-
资助金额:$25.57万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
-
依托单位:
ORGANIZATION OF THE AXON MEMBRANE
-
批准号:3414534
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项目类别:
-
资助金额:$24.5万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
-
依托单位:
MOLECULAR CYTOLOGY OF INTERMEDIATE FILAMENTS IN NERVE
-
批准号:3412705
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项目类别:
-
资助金额:$1.41万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
-
依托单位:
ORGANIZATION OF THE AXON MEMBRANE
-
批准号:3414532
-
项目类别:
-
资助金额:$28.48万
-
财政年份:1990
-
负责人:KIMON J ANGELIDES
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依托单位:
STRUCTURAL/KINETIC/CELLULAR MAPPING--NA+ CHANNEL
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批准号:3075020
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项目类别:
-
资助金额:$6.37万
-
财政年份:1987
-
负责人:KIMON J ANGELIDES
-
依托单位:
STRUCTURAL/KINETIC/CELLULAR MAPPING--NA+ CHANNEL
-
批准号:3075019
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项目类别:
-
资助金额:$6.23万
-
财政年份:1987
-
负责人:KIMON J ANGELIDES
-
依托单位:
STRUCTURAL/KINETIC/CELLULAR MAPPING--NA+ CHANNEL
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批准号:3075018
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项目类别:
-
资助金额:$5.26万
-
财政年份:1987
-
负责人:KIMON J ANGELIDES
-
依托单位:
STRUCTURAL/KINETIC/CELLULAR MAPPING--NA+ CHANNEL
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批准号:3075016
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项目类别:
-
资助金额:$5.05万
-
财政年份:1987
-
负责人:KIMON J ANGELIDES
-
依托单位:
STRUCTURAL/KINETIC/CELLULAR MAPPING--NA+ CHANNEL
-
批准号:3075017
-
项目类别:
-
资助金额:$5.28万
-
财政年份:1987
-
负责人:KIMON J ANGELIDES
-
依托单位:
STRUCTURAL, KINETIC, AND CELLULAR MAPPING OF THE NAT+ CH
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批准号:3409347
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项目类别:
-
资助金额:$9.25万
-
财政年份:1986
-
负责人:KIMON J ANGELIDES
-
依托单位:
STRUCTURAL, KINETIC, AND CELLULAR MAPPING OF THE NAT+ CH
-
批准号:3409344
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项目类别:
-
资助金额:$13.88万
-
财政年份:1986
-
负责人:KIMON J ANGELIDES
-
依托单位:
STRUCTURAL, KINETIC, AND CELLULAR MAPPING OF THE NAT+ CH
-
批准号:3409345
-
项目类别:
-
资助金额:$12.21万
-
财政年份:1986
-
负责人:KIMON J ANGELIDES
-
依托单位:
STRUCTURAL, KINETIC, AND CELLULAR MAPPING OF THE NAT+ CH
-
批准号:3409346
-
项目类别:
-
资助金额:$12.51万
-
财政年份:1986
-
负责人:KIMON J ANGELIDES
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依托单位:
海外基金