DOPAMINE D2-RECEPTOR MEDIATED SIGNAL TRANSDUCTION
多巴胺 D2 受体介导的信号转导
基本信息
- 批准号:3414876
- 负责人:
- 金额:$ 11.83万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1989
- 资助国家:美国
- 起止时间:1989-08-01 至 1993-07-31
- 项目状态:已结题
- 来源:
- 关键词:ADP ribosylation adenylate cyclase biological signal transduction calcium flux calmodulin cyclic AMP dopamine dopamine receptor drug administration routes electrophysiology embryo /fetus tissue /cell culture enzyme induction /repression enzyme substrate laboratory rat neoplastic cell culture for noncancer research neurotransmitters pertussis toxin phosphatidylinositols second messengers stereotaxic techniques
项目摘要
The overall goal of this project is to explore signal transduction
mechanisms for dopamine acting through D2-receptors, in the belief that
this may elucidate the role played by these receptors in regulating
neuronal activity. Consequently, the present proposal takes two
approaches - first, to establish a model cell system in which dopamine's
role in modulating the intracellular second messengers, cAMP and [Ca2+]i
can be delineated and secondly, to investigate the electrophysiological
consequences of a biochemical perturbation of D2-receptor mediated signal
transduction in striatum. The model system to be evaluated will be a
neuroblastoma x Chinese hamster embryonic brain explant hybrid cell line
(NCB-20), which expresses receptors for dopamine, serotonin, bradykinin,
opiates and acetylcholine. The first group of experiments will determine
whether D2-receptors inhibit adenylate cyclase in NCB-20 cells and
whether these receptors also may perturb [Ca2+]i mobilization in these
cells, by measuring D2-receptor effects on phosphatidylinositol
metabolism and [Ca2+]i mobilization. In the second group of experiments,
conditions will be optimized for the intrastriatal injection of pertussis
toxin, so that a significant portion of the pertussis toxin substrates
are ADP-ribosylated; a determination will be made on the effectiveness
with which this treatment eliminates receptor-mediated inhibition of
adenylate cyclase; subsequently, receptor-mediated alterations in
electrophysiological properties will be examined in these animals
compared to sham-injected controls.
这个项目的总体目标是探索信号转导
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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DERMOT M COOPER其他文献
DERMOT M COOPER的其他文献
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{{ truncateString('DERMOT M COOPER', 18)}}的其他基金
REGULATION OF ADENYLYL CYCLASES BY INTRACELLULAR CALCIUM
细胞内钙对腺苷酸环化酶的调节
- 批准号:
2292669 - 财政年份:1997
- 资助金额:
$ 11.83万 - 项目类别:
GTP REGULATORY PROTEINS IN PITUITARY-DERIVED GH3 CELLS
垂体来源的 GH3 细胞中的 GTP 调节蛋白
- 批准号:
3023149 - 财政年份:1990
- 资助金额:
$ 11.83万 - 项目类别:
INTRACELLULAR CALCIUM CONTROL OF CAMP SYNTHESIS
CAMP 合成的细胞内钙控制
- 批准号:
2266902 - 财政年份:1989
- 资助金额:
$ 11.83万 - 项目类别:
INTRACELLULAR CALCIUM CONTROL OF CAMP SYNTHESIS
CAMP 合成的细胞内钙控制
- 批准号:
2379655 - 财政年份:1989
- 资助金额:
$ 11.83万 - 项目类别:
DOPAMINE D2-RECEPTOR MEDIATED SIGNAL TRANSDUCTION
多巴胺 D2 受体介导的信号转导
- 批准号:
3414872 - 财政年份:1989
- 资助金额:
$ 11.83万 - 项目类别:
INTRACELLULAR CALCIUM CONTROL OF CAMP SYNTHESIS
CAMP 合成的细胞内钙控制
- 批准号:
2669002 - 财政年份:1989
- 资助金额:
$ 11.83万 - 项目类别:
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