VIDEO RATE CALCIUM IMAGING FACILITY

美国国立卫生研究院资助的八名研究人员要求提供资金，以建立一个强大而灵活的成像系统，该系统配备了膜片钳功能和快速溶液转换系统。主要的需求是使用荧光指示器以视频速率测量细胞内钙离子浓度，尽管也将测量其他离子。所有这些项目都直接来自调查人员目前资助的项目。健康科学中心没有这样的系统。大约一半的调查人员熟悉其他机构的此类设备。其他人敏锐地意识到将这种分析应用于他们的调查所产生的好处。医学院院长和三位系主任高度致力于建立这一设施。该设施不仅将使一组富有成效的研究人员继续做出有洞察力的贡献，而且将成为丹佛地区志同道合的细胞信号研究人员的重点。

Funds are requested by eight NIH-funded investigators to establish a powerful and flexible imaging system equipped with patch clamp capability and a rapid solution change system. The primary need is to measure intracellular Calcium ion concentration using fluorescent indicators at video rates, although other ions will also be measured. All of the projects arise directly from the investigators' currently funded projects. There is no such system at the Health Sciences Center. Approximately half of the investigators are familiar with such equipment from other institutions. The others have been acutely aware of the benefits arising from the application of such analysis to their investigations. The Dean of the School of Medicine and three department Chairpersons are highly committed to establishing this facility. The facility will serve not only to allow continued insightful contributions from a productive group of investigators, but it will serve as a focus for similarly minded investigators of cellular signaling in the Denver area.