VIDEO RATE CALCIUM IMAGING FACILITY

视频钙成像设备

• 批准号: 2777395
• 负责人: DERMOT M COOPER
• 金额: $ 27.7万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2777395
• 关键词: bioimaging /biomedical imaging; biomedical equipment; biomedical equipment purchase; calcium indicator; calcium ion; video microscopy; voltage /patch clamp

Funds are requested by eight NIH-funded investigators to establish a powerful and flexible imaging system equipped with patch clamp capability and a rapid solution change system. The primary need is to measure intracellular Calcium ion concentration using fluorescent indicators at video rates, although other ions will also be measured. All of the projects arise directly from the investigators' currently funded projects. There is no such system at the Health Sciences Center. Approximately half of the investigators are familiar with such equipment from other institutions. The others have been acutely aware of the benefits arising from the application of such analysis to their investigations. The Dean of the School of Medicine and three department Chairpersons are highly committed to establishing this facility. The facility will serve not only to allow continued insightful contributions from a productive group of investigators, but it will serve as a focus for similarly minded investigators of cellular signaling in the Denver area.

八名NIH资助的研究人员要求资金建立一个强大而灵活的成像系统，配备膜片钳功能和快速更换溶液系统。 主要需要是使用荧光指示剂以视频速率测量细胞内钙离子浓度，尽管也将测量其他离子。所有这些项目都直接来自调查员目前资助的项目。健康科学中心没有这样的系统。大约一半的调查人员熟悉其他机构的这种设备。 其他缔约国敏锐地意识到将这种分析应用于其调查所带来的好处。 医学院院长和三位系主任高度致力于建立这一设施。 该设施不仅将允许一个富有成效的研究小组继续做出有见地的贡献，而且将成为丹佛地区具有类似想法的细胞信号研究人员的重点。