SAXS Core

SAXS核心

• 批准号: 10926637
• 负责人: Yun Xing m wang
• 金额: $ 59.07万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10926637
• 关键词: CCR; COVID-19 pandemic; Collaborations; Communities; Complex; Core Facility; DNA; Data; Data Analyses; Data Collection; Experimental Designs; Extramural Activities; Goals; International; Laboratories; Membrane Lipids; Mission; Nucleic Acids; Persons; Preparation; Principal Investigator; Proteins; RNA; Research; Research Personnel; Research Project Grants; Research Subjects; Sampling; Services; Structure; United States National Institutes of Health; Virion; Waxes; beamline; biomacromolecule; experimental study; instrument; operation; prevent; pup; tutoring

The mission of the SAXS Core facility is to provide support to research projects from CCR principal investigators (PIs), NIH intramural PIs, and extramural academic research groups/laboratories. The support includes providing routine access to the APS PUP SAXS/WAXS beamline and in-house SAXS instrument, and expertise in experimental design, data collection, processing, analysis, and interpretation. Our main focus is to determine the structure of biomacromolecules and their complexes in solution. The research subjects includes but not limited to structural studies of nucleic acids, proteins, protein assemblies, virus particles, lipid membranes, protein/DNA, and protein/RNA complexes. The COVID-19 pandemic has had a negative impact on the operation of the SAXS facility, because of laboratory shutdowns nationwide that prevents in-lab sample preparation and SAXS experiment for most of 2021. In-person SAXS experiments were only allowed to start by the SAXS beamline in May 2022.

SAXS核心设施的使命是为CCR主要研究者（PI）、NIH校内PI和校外学术研究小组/实验室的研究项目提供支持。该支持包括提供对APS PUP SAXS/WAXS光束线和内部SAXS仪器的常规访问，以及实验设计，数据收集，处理，分析和解释方面的专业知识。我们的主要重点是确定生物大分子及其复合物在溶液中的结构。研究课题包括但不限于核酸、蛋白质、蛋白质组装体、病毒颗粒、脂质膜、蛋白质/DNA和蛋白质/RNA复合物的结构研究。2019冠状病毒病疫情对SAXS设施的运营产生负面影响，原因是全国范围内的实验室关闭，导致2021年大部分时间无法进行实验室内的样本制备和SAXS实验。现场SAXS实验仅允许在2022年5月通过SAXS光束线开始。

项目成果

The low-resolution structural models of hepatitis C virus RNA subdomain 5BSL3.2 and its distal complex with domain 3'X point to conserved regulatory mechanisms within the Flaviviridae family.

• DOI: 10.1093/nar/gkac061
• 发表时间: 2022-02-28
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Castillo-Martínez J;Fan L;Szewczyk MP;Wang YX;Gallego J
• 通讯作者: Gallego J