Pacific Northwest Center for Cryo-EM

摘要 冷冻电子显微镜 (cryo-EM) 从近原子成像技术彻底改变了生物医学成像 通过单粒子冷冻电镜对单个生物分子进行分辨率可视化，以定义 使用冷冻电子断层扫描（cryo-ET）原位大分子复合物。这场革命已经被推动 主要归功于用于收集冷冻电镜图像的探测器和计算机软件的进步 用于图像分析和标本重建以及显微镜本身的程序。 因为支撑冷冻电镜的技术既昂贵又在短时间内取得了巨大进步 在一段时间内，许多研究小组可以从将冷冻电镜纳入其生物医学研究中受益 既无法获得冷冻电镜技术，也没有接受过使用该技术的培训。解决访问问题 为了配合冷冻电镜技术和教育，我们将创建太平洋西北冷冻电镜中心。 PNCC 将 是俄勒冈健康与科学大学 (OHSU) 和太平洋西北大学之间的合作成果 国家实验室（PNNL）。通过利用 OHSU 在高分辨率成像方面的优势，生物 凭借 PNNL 在电子领域的长期经验，开展冷冻电镜样品制备和教育 显微镜、高性能计算和强大的用户设施操作，我们将赋予 PNCC 在单粒子冷冻电镜和冷冻电子断层扫描方面具有出色的能力，以及彻底和完整的 冷冻电镜教育或交叉培训计划。此外，由于 PNCC 已建立合作关系 凭借冷冻电镜技术以及计算机硬件和软件领域的领先地位，PNCC 将处于最前沿 的技术能力。此外，OHSU 参加了由以下机构共同主办的冷冻电镜学校： 显微镜制造商 FEI 将支持 PNCC 的交叉培训计划。 OHSU 和 PNNL 都将 对 PNCC 进行重大投资。 OHSU 将为仪器提供低振动空间，它将 向该中心捐赠一台新显微镜，OHSU 将提供 1900 万美元的额外资金用于太空用途 翻新、购买额外的显微镜以及中心工作人员的支持。与此同时，PNNL 承诺访问其高性能计算机中心、用户程序和高分辨率 电子显微镜。通过汇集两个优势互补的主要研究机构， 加上明智选择的合作和主要的额外支持来源，我们将创建一个国家- 最先进、以用户为中心的生物分子和大分子高分辨率成像中心 集会以及对受过冷冻电镜领域培训的下一代科学家的教育。

SUMMARY Cryo-electron microscopy (cryo-EM) has revolutionized imaging in biomedical science, from near atomic resolution visualization of individual biological molecules by single particle cryo-EM to the definition of macromolecular complexes, in situ, using cryo-electron tomography (cryo-ET). This revolution has been driven largely by advances in the detectors employed to collect the cryo-EM images, in the computer software programs utilized in image analysis and specimen reconstruction, and in the microscopes themselves. Because the technology underpinning cryo-EM is both expensive and has dramatically advanced over a short period of time, many research groups that could benefit from incorporating cryo-EM in their biomedical studies neither have access to cryo-EM technology nor have the training to employ the technology. To address access to cryo-EM technology and education, we will create the Pacific Northwest Center for Cryo-EM. The PNCC will be a collaborative effort between the Oregon Health and Science University (OHSU) and the Pacific Northwest National Laboratory (PNNL). By harnessing the strengths of OHSU in high resolution imaging, biological sample preparation and education in cryo-EM with the long-standing experience of PNNL in electron microscopy, high performance computing and robust user facility operation, we will endow the PNCC with outstanding capabilities in single particle cryo-EM and cryo-ET, as well as with a thorough and complete program in cryo-EM education or cross-training. Moreover, because the PNCC has established collaborations with leaders in cryo-EM technology and in computer hardware and software, the PNCC will be at the forefront of technological capability. Furthermore, the participation of OHSU in a cryo-EM school co-sponsored by the microscope manufacturer, FEI, will bolster the cross-training program of the PNCC. Both OHSU and PNNL will make major investments in the PNCC. OHSU will provide a low vibration space for the instrumentation, it will donate a new microscope to the center and OHSU will provide $19M in additional funding for use in space renovation, acquisition of an additional microscope and support of center staff. At the same time, PNNL has pledged access to their high performance computer centers, their user programs and their high resolution electron microscopes. By bringing together two major research institutions with complementary strengths, together with judiciously chosen collaborations and major additional sources of support, we will create a state- of-the-art, user-focused center for the high resolution imaging of biological molecules and macromolecular assemblies and for the education of the next generation of scientists trained in the field of cryo-EM.