Signal Transduction Mechanisms of Type I IFNs

我们早期的工作确定了转录2的信号换能器和激活因子（STAT2）是促进I型干扰素（IFN）诱导的细胞凋亡的关键介质。这是一个有趣的观察结果，因为ifn是已知的唯一激活STAT2的细胞因子。我们最近的工作已经确定了STAT2中一个敏感的保守区域，可以决定IFN刺激后的细胞命运。在我们的细胞系模型中，为了响应ifn - α治疗，在STAT2的Src同源区域2 （SH2）域中引入突变，使肿瘤细胞仅在表达野生型STAT2时才发生凋亡；否则，细胞只会被这种细胞因子抑制生长。根据我们的研究，我们得出结论，这种单一氨基酸的变化延长了STAT1和STAT2之间的物理相互作用。这就使得STAT异源二聚体增加了其在细胞核中的持续时间，同时增加了ifn刺激基因的转录水平。这些发现促使我们仔细检查在12%的人群中检测到的STAT2单核苷酸多态性（SNP）。这个SNP （M594I）是非同义的，在STAT2的SH2结构域中发现。我们测量了它的转录功能和I型IFN对野生型STAT2的生物学反应。我们的研究表明，这种SNP增强了I型ifn的抗增殖作用。更有趣的是，已知被ifn型抑制生长的肿瘤细胞，当它们表达这种SNP时，变得容易受到这种细胞因子的凋亡效应的影响。为了建立这种SNP与干扰素治疗之间的关联，我们分析了一组接受干扰素治疗以控制病毒感染的丙型肝炎患者。STAT2 M594I杂合子患者对IFN治疗的反应优于STAT2纯合子患者。总的来说，我们的研究结果强烈表明，STAT2是I型IFN诱导的细胞凋亡激活的关键成分，特定的STAT2突变可能对接受IFN治疗的患者有益或适得其反。

Our earlier work identified the signal transducer and activator of transcription 2 (STAT2) as a critical mediator in the promotion of type I interferon (IFN)-induced apoptosis. This is an interesting observation as IFNs are the only cytokines known to activate STAT2. Our most recent work has identified a sensitive conserved region in STAT2 that can determine cell fate following IFN stimulation. In our cell line model, in response to IFN-alpha treatment, a mutation introduced in the Src homology region 2 (SH2) domain of STAT2 made tumor cells undergo apoptosis only if they expressed the wild-type form of STAT2; otherwise the cells were only growth arrested by this cytokine. Based on our studies, we concluded that this single amino acid change prolonged the physical interaction between STAT1 and STAT2. This alone allowed the STAT heterodimer to increase its duration in the nucleus while increasing the transcriptional levels of IFN-stimulated genes. These findings prompted us to examine carefully one STAT2 single nucleotide polymorphism (SNP)detected in 12% in the human population. This SNP (M594I) is non-synonymous and is found in the SH2 domain of STAT2. We measured its transcriptional function and consequently type I IFN biological responses against wild-type STAT2. Our studies show that this SNP enhances the antiproliferative effects of type I IFNs. More interestingly, tumor cells that were known to be growth arrested by type IFNs, when they expressed this SNP became susceptible to the apoptotic effects of this cytokine. To establish an association between this SNP and IFN therapy, we analyzed a cohort of hepatitis C patients who had received IFN treatment to control viral infection. Patients heterozygote for STAT2 M594I responded better to IFN therapy than those who were STAT2 homozygote. Collectively, our findings strongly suggest that STAT2 is a critical component in the activation of apoptosis induced by type I IFNs and specific STAT2 mutations may be beneficial or counterproductive to a patient being treated with IFN.