Modular Reagents for Programmable RNA Manipulation by Endogenous Proteins

用于内源蛋白可编程 RNA 操作的模块化试剂

• 批准号: 10605050
• 负责人: Robert Follett Lusi
• 金额: $ 6.87万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-27 至 2026-02-26
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10605050
• 关键词: Ablation; Address; Adenosine; Affect; Base Pair Mismatch; Binding; Biological Assay; Biological Models; Biology; CCND1 gene; Cells; Chemicals; Chemistry; Clinical; Clustered Regularly Interspaced Short Palindromic Repeats; Complementary RNA; Complex; Development; Disease; Down-Regulation; Engineering; Environment; Event; FOXM1 gene; Foundations; Gel; Gene Expression; Genes; Genetic Diseases; Genetic Transcription; Goals; Guide RNA; Health; Human; Immunoprecipitation; In Vitro; Incidence; Intervention; Laboratories; Learning; Length; Libraries; Ligands; Luciferases; Malignant Neoplasms; Mammals; Mediating; Medicine; Messenger RNA; Methylation; Methyltransferase; Modeling; Modification; N-terminal; Nerve Degeneration; Nuclear; Oligonucleotides; Pharmaceutical Preparations; Positioning Attribute; Post-Transcriptional Regulation; Primary carcinoma of the liver cells; Proliferating; Proteins; RNA; RNA Interference; RNA library; Reagent; Reporter; Research; Resources; Scientist; Signal Transduction; Small Interfering RNA; Structure-Activity Relationship; Synthesis Chemistry; System; Therapeutic; Transcript; Translations; Untranslated RNA; Validation; Work; chemical synthesis; design; experimental study; falls; in vivo; member; novel; posttranscriptional; prevent; recruit; side effect; skills; small molecule; tool; transcriptome sequencing

PROJECT SUMMARY: New strategies to targeted difficult-to-drug diseases such as cancers, neurodegeneration, and genetic disorders are urgently needed. RNA manipulation is an emerging, therapeutically attractive paradigm which allows target intervention orthogonal to drugging proteins and without the permanence of gene editing. A variety of tools for RNA manipulation have been developed, but they rely on ribonuclear proteins, which are difficult to deliver in vivo or are limited in scope of effect. This proposal aims to develop RNA-based bifunctional molecules (RBMs) which will consist of an RNA oligonucleotide liked to a small molecule which recruits an effector protein, and will enable modular, programmable targeting of RNA with a variety of manipulations. The proposed mechanism for RBMs is based on small interfering RNA (siRNA) oligonucleotides which are widely used as research tools and have resulted in multiple approved therapeutics. In cells, siRNAs are loaded into Argonaute (AGO) proteins which are part of the RNA silencing complex (RISC). AGO then guides RISC to mRNA targets complementary to its loaded siRNA guide, which are cleaved upon binding, resulting in translational silencing. The oligonucleotide portion of RBMs will function much like siRNAs but will feature key mismatches with the target RNA. Such mismatches have been shown to oblate the cleavage activity of RISC while maintaining target binding. Target binding will induce proximity between the target RNA and an effector protein recruited by the small molecule ligand of the RBM, allowing the effector to act on the target. I will synthesize a small library of RBMs with variable linker lengths and positions, and verify that they can interact with AGO and a model effector protein in vitro (Aim 1). Next, I will use AGO pulldown to show that these interactions can be recapitulated in cells, then use two model systems to show that RBMs can enable post-transcriptional control of mRNA targets (Aim 2). Finally, I will show that RBMs targeting nuclear, long non-coding RNAs can enable control of gene expression (Aim 3). Overall, this will create a platform in which the siRNA paradigm is expanded to enable a much wider variety of manipulations, which will enable novel research tools and therapies. This project will use my existing skills in synthetic chemistry as a foundation and then allow me to branch out in the field of chemical biology. The laboratory of my sponsor, Prof. Steven Banik, is a supportive research environment which will enable me to successfully learn the new skills required to execute this proposal. Prof. Banik is a member of Stanford's Chemistry Engineering and Medicine for Human Health (ChEM-H), a highly collaborative and interdisciplinary institute. Stanford and ChEM-H will afford me all necessary research resources, a variety of opportunities for professional development, and the opportunity to work with, and learn from many, different scientists.

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