Biomarker Characterization in Patients with Subarachnoid Hemorrhage

迟发性缺血性神经功能障碍与蛛网膜下腔动脉瘤样出血和血管痉挛有关。识别预测这些事件发生的蛋白质可能会通过更早地识别和治疗血管痉挛来改善预后。 我们评估了40名发生蛛网膜下腔出血的患者，并在约翰·霍普金斯大学进行了一项IRB批准的研究。他们的血液已经通过使用多重阵列和基于高密度抗体的阵列来识别候选蛋白质的方法进行了筛选，这些阵列询问是否存在500多个炎症标志物。已经确定了30个在蛛网膜下腔出血患者中差异表达的潜在标记物。其中一些标记物仅在入院时存在，可能与随后血管痉挛的发展有关。同样，我们已经确定了一些只在没有发生血管痉挛的患者中存在的蛋白质。我们正在确认这些最初的研究，这些研究是半定量的，并用额外的定量来验证最初的观察结果。此外，我们正在招募更多的患者来证实最初的观察结果。

Delayed ischemic neurological deficits are associated with subarachnoid aneurysmal hemorrhage and vasospasm. Identifying proteins that predict the occurrence of these events may improve outcomes through earlier recognition and treatment of vasospasm. We have evaluated forty patients who have developed subarachnoid hemorrhage and were enrolled an IRB-approved study at Johns Hopkins University. Their blood has been screened with methods to identify candidate proteins using multiplex arrays and high density antibody based arrays that interrogate for the presence of over 500 inflammatory markers. Thirty potential markers have been identified that are differentially expressed in patients with subarachnoid hemorrhage. Some of these markers are only present at the time of hospital admission and may be associated with the subsequent development of vasospasm. Similarly, we have identified some proteins that are only present in patients who do not develop vasospasm. We are confirming these initial studies that are semiquantitive with additional quantative to validate the initial observations. In addition, we are enlisting additional patients to confirm the original observations.