Biomarker Characterization in Patients with Subarachnoid Hemorrhage

蛛网膜下腔出血患者的生物标志物特征

• 批准号: 7733621
• 负责人: ANTHONY F. SUFFREDINI
• 金额: $ 3.78万
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7733621
• 关键词: Admission activity; Affect; Age; Aneurysmal Subarachnoid Hemorrhages; Angiography; Angioplasty; Antibodies; Biological Assay; Biological Markers; Blood; Brain Infarction; Cerebrospinal Fluid; Clinical; Constriction procedure; Deterioration; Development; Diagnosis; Early identification; Economic Burden; Enrollment; Event; Fractionation; Goals; Hemodilution; Hemorrhage; Hospitals; Human; Immunoassay; Incidence; Inflammatory; Institution; Intracranial Aneurysm; Invasive; Mass Spectrum Analysis; Medical; Methods; Neurologic; Older Population; Outcome; Patients; Procedures; Protein Array; Proteins; Research Ethics Committees; Resolution; Risk; Rupture; Sampling; Screening procedure; Site; Staging; Subarachnoid Hemorrhage; Techniques; Time; Two-Dimensional Gel Electrophoresis; Universities; Vasospasm; base; burden of illness; cohort; day; density; digital; experience; improved; prevent; surface enhanced laser desorption ionization; vasoconstriction

Delayed ischemic neurological deficits are associated with subarachnoid aneurysmal hemorrhage and vasospasm. Identifying proteins that predict the occurrence of these events may improve outcomes through earlier recognition and treatment of vasospasm. We have evaluated forty patients who have developed subarachnoid hemorrhage and were enrolled an IRB-approved study at Johns Hopkins University. Their blood has been screened with methods to identify candidate proteins using multiplex arrays and high density antibody based arrays that interrogate for the presence of over 500 inflammatory markers. Thirty potential markers have been identified that are differentially expressed in patients with subarachnoid hemorrhage. Some of these markers are only present at the time of hospital admission and may be associated with the subsequent development of vasospasm. Similarly, we have identified some proteins that are only present in patients who do not develop vasospasm. We are confirming these initial studies that are semiquantitive with additional quantative to validate the initial observations. In addition, we are enlisting additional patients to confirm the original observations.

迟发性缺血性神经功能缺损与蛛网膜下腔动脉瘤出血和血管痉挛有关。识别预测这些事件发生的蛋白质可以通过早期识别和治疗血管痉挛来改善结果。 我们评估了 40 名出现蛛网膜下腔出血的患者，并在约翰霍普金斯大学参加了 IRB 批准的研究。他们的血液经过筛选，使用多重阵列和基于高密度抗体的阵列来识别候选蛋白质，这些阵列可询问是否存在 500 多种炎症标记物。已鉴定出 30 种潜在标记物，这些标记物在蛛网膜下腔出血患者中存在差异表达。其中一些标志物仅在入院时出现，可能与随后发生的血管痉挛有关。同样，我们还发现了一些仅存在于未发生血管痉挛的患者中的蛋白质。我们正在确认这些半定量的初步研究，并通过额外的定量来验证初步观察结果。此外，我们正在招募更多患者来证实最初的观察结果。