Detection of Respiratory Viruses in Upper and Lower Respiratory Tract Specimens

多达100名年龄在12岁及以上的男性或女性受试者已经接受了支气管镜检查以采集BAL样本，并将在BAL样本上订购呼吸道病毒聚合酶链式反应，作为他们在NIH临床中心持续护理的一部分，他们将有资格纳入这项研究。NP拭子样本已通过使用薄膜阵列检测进行验证。NP拭子和BAL样本都将在CRIS中订购呼吸道病毒聚合酶链式反应(FilmArray)分析，样本将由实验室医学系微生物学处的医疗技术员处理和测试。这两个样本的结果将在CRIS中报告。美国疾病控制中心关于采集NP拭子样本的正确技术的说明载于附录A。为了解决结果不一致的问题，结果有冲突的NP和BAL样本将在NIAID传染病实验室病毒致病和进化分部进行第二次多重聚合酶链式反应(Luminex Fast RVP)检测。这些结果将仅用于研究目的。 有关研究参与者的人口统计和临床数据(包括但不限于实验室结果、微生物学数据和放射学结果)将从CRIS中提取，并与薄膜阵列分析的结果相关联。多余的NP拭子或BAL样本将被储存起来，供研究人员未来使用。

Up to 100 male or female subjects age 12 and above who are already undergoing bronchoscopy for the collection of a BAL specimen and who will have respiratory virus PCR ordered on the BAL specimen as part of their ongoing care at the NIH Clinical Center will be eligible for inclusion in this study. NP swab specimens have been validated for testing using the FilmArray assay. Both the NP swab and the BAL specimens will have the respiratory virus PCR (FilmArray) assay ordered in CRIS and the specimens will be processed and tested by the medical technologists in the Microbiology Service of the Department of Laboratory Medicine. Results from both specimens will be reported in CRIS. Instructions from the Centers for Disease Control on the proper technique for collecting NP swab specimens are presented in Appendix A. To resolve discrepant results, NP and BAL specimens with conflicting results will be tested by a second multiplex PCR (Luminex Fast RVP) in the Laboratory of Infectious Diseases, Viral Pathogenesis and Evolution Branch, NIAID. These results will only be used for research purposes. Demographic and clinical data (including but not limited to laboratory results, microbiologic data, and radiology results) on study participants will be abstracted from CRIS and correlated with results from the FilmArray assay. Excess NP swab or BAL samples will be stored for potential future use by study investigators.