Effects of Glucocorticoids or Mineralocorticoids on Endothelial Function

糖皮质激素或盐皮质激素对内皮功能的影响

• 批准号: 7593150
• 负责人: ANTHONY F. SUFFREDINI
• 金额: $ 11.99万
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7593150
• 关键词: Acute; Adjuvant Therapy; Adrenal Cortex Hormones; Aftercare; Aldosterone; Blood Vessels; Cessation of life; Clinical Trials; Condition; Data; Development; Dexamethasone; Dose; Endothelial Cells; Endotoxins; Gene Amplification; Gene Expression; Glucocorticoids; Goals; Healthcare; Hormones; Human; Hydrocortisone; Hypotension; Immunosuppression; Infection; Inflammation; Inflammatory; Inflammatory Response; Injury; Ischemia; Lead; Life; Mediator of activation protein; Meta-Analysis; Methods; Microarray Analysis; Mineralocorticoids; Morbidity - disease rate; Organ failure; Outcome; Patients; Polymerase Chain Reaction; Preparation; Research Personnel; Resolution; Risk; Role; Sepsis; Septic Shock; Shock; Signal Pathway; Signaling Pathway Gene; Steroids; Therapeutic immunosuppression; Time; Tissues; Vasodilation; Vasodilation disorder; animal data; clinical effect; day; in vitro Model; insight; microbial; mortality; pathogen; secondary infection

We have assessed the effects of time and dose of mineralocorticoids and glucocorticoids in an in vitro model of inflammation. Our methods include multiplex arrays, gene amplification with polymerase chain reaction, and microarray analysis. Dexamethasone (a glucocorticoid) and aldosterone (a mineralocorticoid) have fundamentally different inflammatory modulating effects on endothelial cells that are activated with endotoxin. We are currently assessing interactions of these molecules on signaling pathways and gene expression. These data will help to provide some insights into their clinical effects when used in patients with severe hypotension from septic shock.

我们已经评估了盐皮质激素和糖皮质激素在体外炎症模型中的时间和剂量的影响。我们的方法包括多重阵列，基因扩增与聚合酶链反应，和微阵列分析。地塞米松（一种糖皮质激素）和醛固酮（一种盐皮质激素）对内毒素激活的内皮细胞具有根本不同的炎症调节作用。我们目前正在评估这些分子对信号通路和基因表达的相互作用。 这些数据将有助于提供一些见解，他们的临床效果时，用于严重低血压患者感染性休克。