Studies on CDNF: a new class of neurotrophic proteins

CDNF的研究：一类新的神经营养蛋白

• 批准号: 7733849
• 负责人: Barry J Hoffer
• 金额: $ 64.58万
• 依托单位: NATIONAL INSTITUTE ON DRUG ABUSE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7733849
• 关键词: Baltimore; Bilateral; Class; Development; Disruption; Dopamine; Future; Injection of therapeutic agent; Laboratories; Lesion; Midbrain structure; Mitochondria; Modeling; Mus; Mutant Strains Mice; Nerve Growth Factors; Neurotoxins; Oxidopamine; Parkinson Disease; Proteins; Rattus; Reagent; Research; Rodent Model; System; Testing; Transgenic Mice; Transgenic Model; Week; base; dopaminergic neuron; in vivo; mature animal; mitopark mouse; mouse model; neurotrophic factor; null mutation; respiratory enzyme; vector

We study effects of CDNF protein in the rat unilateral maximal lesion PD 6-OHDA model. CDNF is administered prior to, and two weeks after, intracranial injection of 6-OHDA into the ascending DA bundle.We study effects of CDNF protein in the mouse bilateral PD model induced by parental injection of MPTP. We study effects of CDNF protein in the MitoPark mouse model. We are developing a mouse CDNF null mutation and will study development of the midbrain DA system. We will generate AAV-CDNF and in order to test effects in the 6-OHDA rat model and in the MPTP and MitoPark mouse models. In vivo tests of such a vector, if successfully generated, will be carried out in future research.

我们研究了CDNF蛋白在大鼠单侧最大损伤PD 6-OHDA模型中的作用。 在6-OHDA颅内注射到DA上行束之前和两周后给予CDNF，我们研究了CDNF蛋白在由父母注射MPTP诱导的小鼠双侧PD模型中的作用。我们研究了CDNF蛋白在MitoPark小鼠模型中的作用。我们正在开发小鼠CDNF无效突变，并将研究中脑DA系统的发展。我们将产生AAV-CDNF，并且为了测试在6-OHDA大鼠模型和MPTP和MitoPark小鼠模型中的效果。 这种载体的体内试验如果成功产生，将在今后的研究中进行。