Prevention and Treatment of Oral Biofilms and Related Oral Disease by Soluble Chi

描述（由申请人提供）：仍然需要安全有效，预防性和治疗性的治疗方法来控制牙菌斑中的细菌数量和治疗牙周病。我们已经开发了天然生物聚合物的衍生物，在聚集广泛的细菌和防止生物膜的粘附方面具有明显的有效性。技术创新在于增强这些分子固有的相互作用特性，并通过破坏细菌的粘附和内聚，开发出非常低剂量、持续的预防效果和治疗方法，以减少口腔疾病的发生。本应用的目的是探索利用这些新合成的不同大小和电荷的可溶性壳聚糖衍生物，通过游离细菌的聚集来防止细菌定植，并通过减少牙菌斑的内聚和粘附来治疗口腔生物膜。一旦了解了壳聚糖衍生物与口腔病原体、菌斑和菌斑形成之间的特定相互作用，我们就可以开发出降低口腔病原体滴度的产品配方，无论是游离的还是生物膜中的。本提案专门针对一种口腔冲洗产品的开发，以预防和治疗牙齿疾病，包括广泛的治疗应用，包括龋齿，牙周炎和牙髓炎。我们的新药物干扰微生物定植和生长，是本建议的基础。本研究将探索利用新合成的可溶性壳聚糖衍生物作为治疗和预防口腔生物膜（牙菌斑）的药物，通过聚集口腔细菌，侵蚀牙菌斑，防止细菌与牙菌斑和口腔表面的粘附，对天然口腔菌群的影响最小。长期产品将包括通过不同的剂量或治疗方案来减少感染或患病状态下的细菌数量。考虑到壳聚糖衍生物的生物相容性、生物可降解性和有限的抗性机制，其应用前景十分广阔。

DESCRIPTION (provided by applicant): A need remains for safe and effective, prophylactic and therapeutic treatments for controlling bacterial populations in dental plaque and treating periodontal disease. We have developed derivatives of natural biopolymers that have demonstrable effectiveness in clumping a wide spectrum of bacteria and preventing the adhesion of biofilms. The technological innovation lies in enhancing the innate interactive properties of these molecules and developing means to create a very low dose, sustained preventative effect and treatment to reduce the occurrence of oral disease by disrupting bacterial adhesion and cohesion. The objective of this application is to explore the use of these newly synthesized soluble chitosan derivatives of various size and charge to prevent bacterial colonization via aggregation of free bacteria and to treat oral biofilms by reducing plaque cohesion and adhesion. Once the specific interactions between chitosan derivatives and oral pathogens, plaque, and plaque formation are understood, we can knowledgably develop product formulations that minimize the titer of oral pathogens, both free and in biofilms. This proposal specifically addresses the development of an oral rinse product to prevent and treat dental disease encompassing broad treatment applications including caries, periodontitis and pulpitis. Our novel agents interfere with microbial colonization and growth and are the basis of this proposal. This study will explore the use of newly synthesized soluble chitosan derivatives as agents to treat and prevent oral biofilms (dental plaque) with minimal affects on natural oral flora by aggregation of oral bacteria, erosion of plaque and prevention of adhesion of bacteria to plaque and oral surfaces. Longer term products will include treatments to reduce the bacterial populations in infected or diseased states via different dosing or treatment regimes. Given the biocompatibility, biodegradability and limited mechanism to resistance for this clumping mechanism, the applications these chitosan derivatives are expected to be far reaching. PUBLIC HEALTH RELEVANCE: This proposal examines the use of biopolymer derivatives that have demonstrable effectiveness in clumping a wide spectrum of bacteria and preventing the adhesion of biofilms for use in controlling oral biofilms. These products will provide low dose, highly effective, biocompatible, sustained preventative effect and treatment to reduce the occurrence of oral disease by disrupting bacterial adhesion and cohesion.