Prevention and Treatment of Oral Biofilms and Related Oral Disease by Soluble Chi

描述（由申请人提供）：仍然需要用于控制牙菌斑中的细菌群体和治疗牙周病的安全有效的预防性和治疗性治疗。我们已经开发出天然生物聚合物的衍生物，这些衍生物在聚集广谱细菌和防止生物膜粘附方面具有明显的效果。技术创新在于增强这些分子的固有相互作用特性，并开发方法以产生非常低的剂量，持续的预防效果和治疗，通过破坏细菌粘附和凝聚来减少口腔疾病的发生。本申请的目的是探索这些新合成的具有各种尺寸和电荷的可溶性壳聚糖衍生物的用途，以通过游离细菌的聚集来防止细菌定植，并通过减少牙斑凝聚和粘附来治疗口腔生物膜。一旦了解了壳聚糖衍生物与口腔病原体、牙菌斑和牙菌斑形成之间的特定相互作用，我们就可以有知识地开发产品配方，使口腔病原体的滴度最小化，无论是游离的还是生物膜中的。本提案专门针对预防和治疗牙科疾病的口腔冲洗产品的开发，包括广泛的治疗应用，包括龋齿、牙周炎和牙髓炎。我们的新型试剂干扰微生物的定植和生长，是这项提议的基础。本研究将探索使用新合成的可溶性壳聚糖衍生物作为治疗和预防口腔生物膜（牙菌斑）的药物，通过口腔细菌的聚集、牙菌斑的侵蚀和防止细菌粘附到牙菌斑和口腔表面，对天然口腔植物群的影响最小。长期产品将包括通过不同的剂量或治疗方案减少感染或患病状态下的细菌种群的治疗。鉴于生物相容性，生物降解性和有限的机制，以抵抗这种结块机制，这些壳聚糖衍生物的应用预计将是深远的。 公共卫生相关性：该提案考察了生物聚合物衍生物的用途，所述生物聚合物衍生物在聚集广谱细菌和防止生物膜粘附方面具有明显的有效性，用于控制口腔生物膜。这些产品将提供低剂量、高效、生物相容、持续的预防效果和治疗，通过破坏细菌粘附和凝聚来减少口腔疾病的发生。

DESCRIPTION (provided by applicant): A need remains for safe and effective, prophylactic and therapeutic treatments for controlling bacterial populations in dental plaque and treating periodontal disease. We have developed derivatives of natural biopolymers that have demonstrable effectiveness in clumping a wide spectrum of bacteria and preventing the adhesion of biofilms. The technological innovation lies in enhancing the innate interactive properties of these molecules and developing means to create a very low dose, sustained preventative effect and treatment to reduce the occurrence of oral disease by disrupting bacterial adhesion and cohesion. The objective of this application is to explore the use of these newly synthesized soluble chitosan derivatives of various size and charge to prevent bacterial colonization via aggregation of free bacteria and to treat oral biofilms by reducing plaque cohesion and adhesion. Once the specific interactions between chitosan derivatives and oral pathogens, plaque, and plaque formation are understood, we can knowledgably develop product formulations that minimize the titer of oral pathogens, both free and in biofilms. This proposal specifically addresses the development of an oral rinse product to prevent and treat dental disease encompassing broad treatment applications including caries, periodontitis and pulpitis. Our novel agents interfere with microbial colonization and growth and are the basis of this proposal. This study will explore the use of newly synthesized soluble chitosan derivatives as agents to treat and prevent oral biofilms (dental plaque) with minimal affects on natural oral flora by aggregation of oral bacteria, erosion of plaque and prevention of adhesion of bacteria to plaque and oral surfaces. Longer term products will include treatments to reduce the bacterial populations in infected or diseased states via different dosing or treatment regimes. Given the biocompatibility, biodegradability and limited mechanism to resistance for this clumping mechanism, the applications these chitosan derivatives are expected to be far reaching. PUBLIC HEALTH RELEVANCE: This proposal examines the use of biopolymer derivatives that have demonstrable effectiveness in clumping a wide spectrum of bacteria and preventing the adhesion of biofilms for use in controlling oral biofilms. These products will provide low dose, highly effective, biocompatible, sustained preventative effect and treatment to reduce the occurrence of oral disease by disrupting bacterial adhesion and cohesion.