Engineering industrial fermentations for renewable amino acid coproducts

可再生氨基酸副产品的工程工业发酵

• 批准号: 7612456
• 负责人: J. Mark Weber
• 金额: $ 23.78万
• 依托单位: FERMALOGIC, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7612456
• 关键词: Affect; Amino Acids; Animal Feed; Animal Model; Animal Sources; Animals; Bacteria; Biochemical; Biological Assay; Capital; Chemicals; Cost Savings; Cysteine; Cystine; Databases; Development; Economics; Engineering; Equipment; Erythro; Erythromycin; Escherichia coli; Essential Amino Acids; Family; Fermentation; Figs - dietary; Foundations; Genes; Genetic; Genetic Engineering; Goals; Government regulations; Health; Human; Intention; Knock-out; Knowledge; Letters; Libraries; Licensing; Liquid substance; Lysine; Manufacturer Name; Marketing; Metabolic; Metabolism; Methods; Modeling; Motivation; Mutation; Neurodegenerative Disorders; Operative Surgical Procedures; Organism; Phase; Phase I Clinical Trials; Plasmids; Population; Prion Diseases; Prions; Process; Production; Recovery; Reporting; Reproduction spores; Research; Research Personnel; Resistance; Risk; Route; Saccharopolyspora; Saccharopolyspora erythraea; Screening procedure; Solid; Staging; Stream; Technology; Variant; cost; cysteine desulfurase; dimer; feeding; flasks; improved; interest; lysine analog; manufacturing process; mutant; public health relevance; tool

DESCRIPTION (provided by applicant): Knowledge of amino acid metabolism is critical to human health and has significant industrial applications as well. This study proposes to develop a coproduct method for the production of L-lysine, an essential amino acid of great economic and industrial importance. If successful, the L-lysine produced as a result of this study will be a coproduct of the erythromycin fermentation using Saccharopolyspora erythraea. Currently, L-lysine is produced from dedicated Corynebactium glutamicum fermentations, and as such, has costs associated with its production that are not absorbed through the production of a primary fermentation product. L-lysine produced as a coproduct from the erythromycin fermentation would be substantially less expensive to produce, and large quantities could be produced using this method because the erythromycin fermentation is also a very large-scale process. In addition, this study also proposes to investigate the potential for developing a dedicated fermentation method for L-cysteine production, using an erythromycin non-producing variant of S. erythraea. Until recently there was no need for fermentation-produced L-cysteine; but due to recent government regulations against the use of animal derived L-cysteine, the market for fermentation-produced material has grown significantly. The model organism used in this study is the erythromycin producing organism, S. erythraea, a bacterium that has been the subject of over 50 years of intensive genetic and biochemical research, providing a solid foundation upon which to build commercial processes for amino acid production. Public Health Relevance: Prion diseases, or transmissible spongiform encephalopathies (TSEs), are a family of rare progressive neurodegenerative disorders that affect both humans and animals. Prion diseases are usually rapidly progressive and always fatal. This project aims to reduce the risk of acquiring TSE<s in the population through the development of a biotechnological process for the production of amino acids that are used by humans and are currently obtained from possibly contaminated animal sources.

描述（由申请人提供）：氨基酸代谢的知识对人类健康至关重要，并且具有重要的工业应用。这项研究建议开发一种生产L-赖氨酸的合并方法，L-赖氨酸是一种重要的经济和工业重要性的基本氨基酸。如果成功的话，这项研究产生的L-赖氨酸将是使用Saccharopolyspora reythraea进行红霉素发酵的合并。目前，L-赖氨酸是由专用的Corynebactium Grutamicum发酵产生的，因此具有与其生产相关的成本，这些成本并非因产生主要发酵产品而吸收。从红霉素发酵中产生的l-赖氨酸作为共同生产的生产价格要便宜得多，并且可以使用这种方法生产大量，因为红霉素发酵也是一个非常大的过程。此外，这项研究还建议研究使用红霉素的红霉素非生产变体开发用于L-半胱氨酸产生的专用发酵方法的潜力。直到最近，都不需要发酵产生的L-半胱氨酸。但是，由于最近反对使用动物衍生的L-半胱氨酸的法规，发酵生产材料的市场已大大增长。这项研究中使用的模型有机体是产生红霉素的生物链球菌，这是一种已有50多年的密集遗传和生化研究的细菌，为氨基酸生产提供了商业过程。公共卫生的相关性：prion疾病或可传染性的海绵状脑病（TSES），是一个罕见的渐进神经退行性疾病的家族，影响人类和动物。王室疾病通常是迅速进行的，总是致命的。该项目旨在通过开发人类使用的生物技术过程来降低人群中获得TSE <S的风险，这些过程是人类使用的氨基酸，目前是从可能被污染的动物来源获得的。