Industrial Metabolic Engineering

工业代谢工程

• 批准号: 7537278
• 负责人: J. Mark Weber
• 金额: $ 49.15万
• 依托单位: FERMALOGIC, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-15 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7537278
• 关键词: Actinobacteria class; Actinomyces; Adopted; Affect; Animal Model; Antibiotics; Antineoplastic Agents; Area; Azithromycin; Bacteria; Biochemical Genetics; Businesses; Chemicals; China; Clarithromycin; Croatia; Engineering; Erythromycin; Fermentation; Foundations; Future; Generations; Genes; Genetic; Genetic Models; Genome; Goals; Immunosuppressive Agents; In Vitro; India; Industry; Ketek; Knowledge; Label; Laboratories; Lead; Libraries; Manufacturer Name; Marketing; Medical; Metabolic; Metabolism; Methods; Mexico; Mutagenesis; Mutation; Organism; Phase; Poland; Procedures; Process; Production; Range; Research; Roxithromycin; Saccharopolyspora erythraea; Scientist; Soil; Solid; Technology; Today; base; dirithromycin; flasks; improved; microorganism; miniaturize; mutant; programs; theories

DESCRIPTION (provided by applicant): Industrial metabolic engineering is a method for performing strain improvement in industrial microorganisms by harnessing the power of random in vitro transposition mutagenesis. The organisms we aim to improve in this study are the actinomycetes, a group of soil bacteria that are best known for their ability to produce over two thirds of the worlds naturally derived antibiotics, anticancer agents, and immunosuppressants currently in medical use. The transposon tagging process is powerful not only because of its ability to make strain improvement mutations, but also because it allows strains to be easily reverse engineered to reveal the identity of the affected gene. Once the strain improvement target is identified, new genetic and metabolic knowledge is revealed that can lead to further optimization and extension of the technology to other organisms of medical importance. The model organism used in this study is the erythromycin producing organism, Saccharopolyspora erythraea, a bacterium that has been the subject of over 50 years of intensive genetic and biochemical research, providing a solid foundation upon which to build the fundamentals for the emerging field of metabolic engineering of industrial microorganisms.

描述（由申请人提供）：工业代谢工程是一种通过利用随机体外转座诱变的力量来改善工业微生物的应变方法。我们旨在改善这项研究的生物是放线菌，这是一组土壤细菌，它们以其生产自然衍生的抗生素，抗癌剂和当前医疗用途中的免疫抑制剂的能力而闻名。转座标记过程不仅强大，这不仅是因为其能够改善菌株的突变，而且还因为它允许菌株易于进行逆向设计以揭示受影响基因的身份。一旦确定了菌株的改善靶标，就会发现新的遗传和代谢知识可以导致技术进一步优化和扩展到其他具有医学重要性的生物体。这项研究中使用的模型有机体是产生红霉素的生物体，糖孢子虫红斑，这是一种细菌，已成为50多年的密集遗传和生化研究的主题，为稳固的基础提供了稳固的基础，以建立基本面，以建立工业微型机构代谢工程新兴工程领域的基础知识。