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Novel Assay for Assessing ROS

评估 ROS 的新方法

基本信息

批准号：
7676616
负责人：
Chunqi Li
金额：
$ 19.4万
依托单位：
PHYLONIX PHARMACEUTICALS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2011-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7676616
关键词：
8-hydroxy-2&apos -deoxyguanosine Acids Animals Anterior Antibodies Apoptosis Biological Assay Brain Brain region Cell Proliferation Cell physiology Cessation of life Chronic DNA DNA Damage DNA Modification Process Data Deoxyguanosine Development Disease Dorsal Drug Controls Enzyme-Linked Immunosorbent Assay Evaluation Exhibits Failure Fishes Fluorescence Frequencies Government Imagery Legal patent Light Location Mammals Measurement Methods Modeling Molecular Mus Necrosis Organ Oxidative Stress Oxygen Persons Pharmaceutical Preparations Pharmacologic Substance Phase Phase I Clinical Trials Play Poison Preclinical Drug Evaluation Process Progressive Disease Reactive Nitrogen Species Reactive Oxygen Species Relative (related person)Research Role Staging Staining method Stains System Testing Time Tissues Toxic effect Tumor Promotion Water Zebrafish base carcinogenesis cell growth cell transformation cost drug development drug mechanism high throughput screening in vivo irradiation luminescence method development novel oxidation public health relevance tumor initiation

项目摘要

DESCRIPTION (provided by applicant): Reactive oxygen species (ROS) are known to contribute as pathogenic factors to the development of chronic progressive diseases at various stages. ROS are also involved in a variety of different cellular processes ranging from apoptosis and necrosis to cell proliferation and carcinogenesis. ROS are well recognized for playing a dual role as both deleterious and beneficial species. They are also capable of inducing DNA damage that eventually may contribute to cell transformation and tumor initiation and are also associated with tumor promotion and progression. ROS also act as primary or secondary messengers to promote cell growth or death. In Phase I research, we will develop a microplate-based zebrafish whole animal assay to assess drug-induced oxidative stress. PUBLIC HEALTH RELEVANCE: Molecular players comprise both reactive oxygen (ROS) and reactive nitrogen species (RNS), which are known to contribute as pathogenic factors to the development of chronic progressive diseases at various stages.

描述（由申请人提供）：已知活性氧（ROS）在慢性进行性疾病的各个阶段作为致病因素发挥作用。ROS还参与多种不同的细胞过程，从细胞凋亡和坏死到细胞增殖和癌变。活性氧具有有益和有害的双重作用。它们还能够诱导DNA损伤，最终可能促进细胞转化和肿瘤的发生，并与肿瘤的促进和进展有关。ROS还作为主要或次要信使促进细胞生长或死亡。在I期研究中，我们将开发一种基于微孔板的斑马鱼全动物实验来评估药物诱导的氧化应激。公共卫生相关性：分子参与者包括活性氧（ROS）和活性氮（RNS），它们被认为是慢性进行性疾病在不同阶段发展的致病因素。