A High-Resolution Differential Mobility Analyzer to Study Biomolecules

描述（由申请人提供）：离子迁移谱法（IMS）是一种强大的分析方法，可用于研究三维结构和化合物化学过程中发生的结构变化，包括簇，合成聚合物和生物分子，如蛋白质和肽。它还用于检测空气中的化学制剂，如爆炸物、化学战剂、麻醉品和有毒工业化学品，并用于研究气溶胶颗粒。该项目旨在满足改进与这些应用程序相关的IMS性能的需求。相对于更常见的基于漂管的IMS，差分IMS是一种具有高占空比和灵敏度的方法。提出了一种比现有差分离子迁移谱仪分辨率更高的新型差分离子迁移谱仪。所提出的光谱仪将采用多缝多通道设计，通过允许缓冲气体的有效层流来提高该技术的分辨率。公共卫生相关性：离子迁移率光谱法（IMS）是一种分离和研究各种分析物（包括生物化合物，如蛋白质和肽）产生的气体离子的三维结构的方法，并研究其结构与化学过程相关的变化。它还用于检测空气中的化学制剂，如爆炸物、化学战剂、麻醉品和有毒工业化学品。我们提出的设计是IMS中的一个新发展，旨在提高该方法的性能，使其各种应用程序受益。这种新颖的设计将同时提高IMS的灵敏度和分辨率。

DESCRIPTION (provided by applicant): Ion mobility spectrometry (IMS) is a powerful analytical method having applications in the investigation of three dimensional structures and the structural changes that occur during chemical processes in compounds including clusters, synthetic polymers and biomolecules such as proteins and peptides. It is also used for the detection of airborne chemical agents such as explosives, chemical warfare agents, narcotics and toxic industrial chemicals and for the study of aerosol particles. This project is aimed to address the need to improve the performance of the IMS as related to these applications. Differential IMS is a method having a high duty cycle and sensitivity with respect to the more common drift-tube based IMS. A novel differential ion mobility spectrometer with a resolution higher than the existing differential ion mobility designs is proposed. The proposed spectrometer will have a multi-slit-multi-channel design to improve the resolution of the technique by allowing for an efficient laminar flow of the buffer gas. PUBLIC HEALTH RELEVANCE: Ion mobility spectrometry (IMS) is a method to separate and study three-dimensional structures of gaseous ions produced by various analytes including biological compounds such as proteins and peptides and to study the changes in their structures as related to chemical processes. It is also used for the detection of airborne chemical agents such as explosives, chemical warfare agents, narcotics and toxic industrial chemicals. Our proposed design is a novel development in the IMS aimed at the need to increase the performance of the method so that its various applications may benefit. The novel design will simultaneously improve the sensitivity and resolution of the IMS.