A High-Resolution Differential Mobility Analyzer to Study Biomolecules

用于研究生物分子的高分辨率微分淌度分析仪

• 批准号: 7611446
• 负责人: Chaminda M. Gamage
• 金额: $ 9.77万
• 依托单位: SCIENCE AND ENGINEERING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7611446
• 关键词: Address; Aerosols; Affect; Area; Biological; Buffers; Carbohydrates; Charge; Chemical Agents; Chemical Structure; Chemical Warfare Agents; Chemicals; Chemistry; Coupled; Coupling; Cyclotrons; Detection; Development; Devices; Diffusion; Drops; Gases; Individual; Investigation; Ions; Lasers; Length; Location; Mass Spectrum Analysis; Measurement; Measures; Methods; Modification; Molecular; Molecular Analysis; Molecular Conformation; Narcotics; Operative Surgical Procedures; Peptides; Performance; Phase; Physiologic pulse; Polymers; Positioning Attribute; Process; Property; Proteins; Research; Resolution; Sampling; Scanning; Science; Series; Shapes; Source; Spectrometry; Spectrum Analysis; Staging; Stream; Structure; Techniques; Time; Tube; analytical method; base; design; detector; electric field; flexibility; improved; instrument; ion mobility; ion source; ionization technique; mass spectrometer; millisecond; novel; particle; pressure; public health relevance; research study; three dimensional structure; time use; toxic industrial chemical; voltage

DESCRIPTION (provided by applicant): Ion mobility spectrometry (IMS) is a powerful analytical method having applications in the investigation of three dimensional structures and the structural changes that occur during chemical processes in compounds including clusters, synthetic polymers and biomolecules such as proteins and peptides. It is also used for the detection of airborne chemical agents such as explosives, chemical warfare agents, narcotics and toxic industrial chemicals and for the study of aerosol particles. This project is aimed to address the need to improve the performance of the IMS as related to these applications. Differential IMS is a method having a high duty cycle and sensitivity with respect to the more common drift-tube based IMS. A novel differential ion mobility spectrometer with a resolution higher than the existing differential ion mobility designs is proposed. The proposed spectrometer will have a multi-slit-multi-channel design to improve the resolution of the technique by allowing for an efficient laminar flow of the buffer gas. PUBLIC HEALTH RELEVANCE: Ion mobility spectrometry (IMS) is a method to separate and study three-dimensional structures of gaseous ions produced by various analytes including biological compounds such as proteins and peptides and to study the changes in their structures as related to chemical processes. It is also used for the detection of airborne chemical agents such as explosives, chemical warfare agents, narcotics and toxic industrial chemicals. Our proposed design is a novel development in the IMS aimed at the need to increase the performance of the method so that its various applications may benefit. The novel design will simultaneously improve the sensitivity and resolution of the IMS.

描述（由申请人提供）：离子迁移谱（IMS）是一种强大的分析方法，可应用于研究化合物（包括簇、合成聚合物和生物分子，如蛋白质和肽）的三维结构和化学过程中发生的结构变化。它还用于检测空气中的化学制剂，如爆炸物、化学战剂、麻醉剂和有毒工业化学品，以及用于研究气溶胶颗粒。该项目旨在满足改进国际监测系统与这些应用有关的性能的需要。差分IMS是相对于更常见的基于漂移管的IMS具有高占空比和灵敏度的方法。提出了一种新的差分离子迁移率谱仪，其分辨率高于现有的差分离子迁移率设计。拟议的光谱仪将有一个多缝多通道的设计，以提高分辨率的技术，允许一个有效的层流的缓冲气体。公共卫生关系：离子迁移谱（IMS）是一种分离和研究由各种分析物（包括生物化合物，如蛋白质和肽）产生的气态离子的三维结构，并研究与化学过程相关的结构变化的方法。它还用于检测空气中的化学制剂，如爆炸物，化学战剂，麻醉品和有毒工业化学品。我们提出的设计是一个新的发展，在IMS的目的是需要提高性能的方法，使其各种应用程序可能受益。这种新颖的设计将同时提高IMS的灵敏度和分辨率。