A High-Resolution Differential Mobility Analyzer to Study Biomolecules

用于研究生物分子的高分辨率微分淌度分析仪

• 批准号: 7611446
• 负责人: Chaminda M. Gamage
• 金额: $ 9.77万
• 依托单位: SCIENCE AND ENGINEERING SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7611446
• 关键词: Address; Aerosols; Affect; Area; Biological; Buffers; Carbohydrates; Charge; Chemical Agents; Chemical Structure; Chemical Warfare Agents; Chemicals; Chemistry; Coupled; Coupling; Cyclotrons; Detection; Development; Devices; Diffusion; Drops; Gases; Individual; Investigation; Ions; Lasers; Length; Location; Mass Spectrum Analysis; Measurement; Measures; Methods; Modification; Molecular; Molecular Analysis; Molecular Conformation; Narcotics; Operative Surgical Procedures; Peptides; Performance; Phase; Physiologic pulse; Polymers; Positioning Attribute; Process; Property; Proteins; Research; Resolution; Sampling; Scanning; Science; Series; Shapes; Source; Spectrometry; Spectrum Analysis; Staging; Stream; Structure; Techniques; Time; Tube; analytical method; base; design; detector; electric field; flexibility; improved; instrument; ion mobility; ion source; ionization technique; mass spectrometer; millisecond; novel; particle; pressure; public health relevance; research study; three dimensional structure; time use; toxic industrial chemical; voltage

DESCRIPTION (provided by applicant): Ion mobility spectrometry (IMS) is a powerful analytical method having applications in the investigation of three dimensional structures and the structural changes that occur during chemical processes in compounds including clusters, synthetic polymers and biomolecules such as proteins and peptides. It is also used for the detection of airborne chemical agents such as explosives, chemical warfare agents, narcotics and toxic industrial chemicals and for the study of aerosol particles. This project is aimed to address the need to improve the performance of the IMS as related to these applications. Differential IMS is a method having a high duty cycle and sensitivity with respect to the more common drift-tube based IMS. A novel differential ion mobility spectrometer with a resolution higher than the existing differential ion mobility designs is proposed. The proposed spectrometer will have a multi-slit-multi-channel design to improve the resolution of the technique by allowing for an efficient laminar flow of the buffer gas. PUBLIC HEALTH RELEVANCE: Ion mobility spectrometry (IMS) is a method to separate and study three-dimensional structures of gaseous ions produced by various analytes including biological compounds such as proteins and peptides and to study the changes in their structures as related to chemical processes. It is also used for the detection of airborne chemical agents such as explosives, chemical warfare agents, narcotics and toxic industrial chemicals. Our proposed design is a novel development in the IMS aimed at the need to increase the performance of the method so that its various applications may benefit. The novel design will simultaneously improve the sensitivity and resolution of the IMS.

描述（由申请人提供）：离子迁移率光谱法（IMS）是一种强大的分析方法，在研究三维结构中的应用以及化合物中化学过程中发生的结构变化以及包括簇，合成聚合物以及蛋白质和肽等化合物的结构变化。它也用于检测机载化学剂，例如炸药，化学战剂，麻醉品和有毒工业化学物质以及气溶胶颗粒的研究。该项目的目的是解决与这些应用程序相关的IMS性能的需求。差异IMS是一种相对于基于漂移管的IMS具有高占空比和灵敏度的方法。提出了一种高于现有差分离子迁移率设计的新型差分离子迁移率。拟议的光谱仪将具有多缝 - 渠道设计，以通过允许有效的缓冲液层流进行改善技术的分辨率。公共卫生相关性：离子迁移率（IMS）是一种分离和研究由各种分析物产生的气态离子的三维结构的方法，包括生物化合物，例如蛋白质和肽，并研究其结构的变化与化学过程有关。它也用于检测机载化学剂，例如炸药，化学战剂，麻醉品和有毒工业化学品。我们提出的设计是IMS的新开发，旨在提高该方法的性能，以便其各种应用可能受益。新颖的设计将同时提高IMS的灵敏度和分辨率。