EMBRYONIC STEM CELL AND EMBRYONIC GERM CELL LINE

项目一至项目三的每一位首席研究人员都是公认的人类和非人类灵长类胚胎专家。 干细胞和生殖细胞。胚胎干细胞和胚胎生殖细胞(“干细胞和生殖细胞”)的核心利用了这一点。 结合专业知识，从而为所有蜂窝网络提供中央质量保证和质量控制实验室 三个项目产生的资源。为支持这三个项目，提出了这一核心的三个具体目标。 目标1：建立和维护人类和非人灵长类胚胎干细胞的核心资源，以供分配给 每个项目和其他核心使用良好的实验室实践文化和经常监测多能性 和非整倍体。目的2：建立和维护非人灵长类胚胎生殖细胞核心资源 使用良好的实验室实践文化和频繁的监测向每个项目和其他核心分发 多能性和非整倍体。目标3(干细胞遗传和表观遗传资源)维护人类和非人类的集合 关于它们的遗传和表观遗传状态的灵长类ES和EG细胞系 (无论是正常还是异常)，用于每个项目和其他岩芯。三个方面的每一个的实现 AIMS将支持这三个项目和其他核心项目，提供体外分子和细胞分析， 产生嵌合体(在非人类灵长类ES和EG细胞的情况下)；以及对发育命运的分析，两者都 体外和体内(后者在涉及动物模型的移植研究中)。通过将专业知识和 三个项目负责人的资源这一核心可最大限度地利用现有资源，减少重复和重叠。

Each of the principal investigators for projects I-III is a recognized expert in human and non-human primate embryonic stem and germ cells. The Embryonic Stem Cell and Embryonic Germ Cell ("Stem and Germ Cell") Core draws on this combined expertise, thereby providing a central quality assurance and quality control laboratory for all cellular resources generated by the three projects. Three specific aims are proposed for this core in support of the three projects. Aim 1: Establish and maintain core resource of human and non-human primate embryonic stem cells for distribution to each of the projects and the other cores using good laboratory practice culture and frequent monitoring of pluripotency and aneuploidy. Aim 2: Establish and maintain core resource of non-human primate embryonic germ cells for distribution to each of the projects and the other cores using good laboratory practice culture and frequent monitoring of pluripotency and aneuploidy. Aim 3 (Stem Cell Genetic and Epigenetic Resource) Maintain sets of human and nonhuman primate ES and EG cell lines that are well-characterized with respect to their genetic and epigenetic status (whether normal or abnormal) for use in each of the projects and the other cores. The fulfillment of each of the three aims will support the three projects and the other cores in providing for in vitro molecular and cellular analysis, generation of chimeras (in the case of non-human primate ES and EG cells); and analysis of developmental fates, both in vitro and in vivo (the latter in transplantation studies involving animal models). By combining the expertise and resources of the three project leaders this core maximizes the available resources and reduces duplication and overlap.