权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

DEVELOPMENTAL BIOLOGY OF THE IMPLANTING MAMMALIAN EMBRYO

哺乳动物胚胎植入的发育生物学

基本信息

批准号：
6151140
负责人：
Roger Arnold Pedersen
金额：
$ 112.62万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-02-01 至 2002-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6151140
关键词：
developmental genetics early embryonic stage embryo implantation gene expression

项目摘要

This Program Project emphasizes the molecular and cellular events of mammalian implantation and placental formation, an essential but little- studied process in early reproduction that has great significance for human health. The purpose of the Program is to define the role of genes that are essential for implantation and thus to achieve a basic understanding of the interactions between the embryo and uterus that establish pregnancy. The Program focuses on the peri-implantation development of the three tissue lineages that mediate implantation and develop into the placenta and fetal membranes, namely, trophoblast, extraembryonic endoderm, and extraembryonic mesoderm. Four projects examine the roles of individual membranes of several superfamilies of genes whose products are implicated in controlling peri-implantation development and implantation. These include the extracellular matrix receptors, or integrins, proteases and their inhibitors, peptide growth factors and their receptors, and HLH and other transcription factors. Project I examines the role of integrin receptors for extracellular matrix in peri-implantation development of trophoblast and primitive endoderm; Project II analyzes the roles of helix-loop-helix transcription factors in regulating human trophoblast invasion; Project III studies the mechanisms regulating growth, cell division and differentiation during morphogenesis of extraembryonic lineages and in the invasive process in vivo; and Project VI analyzes the differentiation and morphogenesis of extraembryonic mesoderm, especially the allantois. The Projects are supported by an Administrative Core, which provides fiscal management, and two research cores, the Morphology/Cytogenetics Core, which provides histological, in situ hybridization, confocal microscopy, and chromosomal analysis services, and the Cell Culture/Targeted Mutagenesis Core, which provides cell culture and genetic transformation services, including all the activities needed for targeted mutagenesis in mice. The proposed studies have major implications for human health problems, including syndromes of infertility and early pregnancy loss, inefficient implantation rates of embryos generated through medically assisted conception, and diseases of the trophoblast such as choriocarcinoma, hydatidiform mole and pre-eclampsia. These problems can ultimately be resolved only through mammalian studies, as undertaken in the Program, which examine the fundamental mechanisms of embryo-maternal interactions.

该计划项目强调分子和细胞事件哺乳动物的植入和胎盘的形成，这是一个基本但很少的- 研究早期繁殖的过程，对人类健康。该计划的目的是定义基因的作用对于植入是必不可少的，从而实现基本的了解胚胎和子宫之间的相互作用确定怀孕。该计划的重点是围植入期介导着床的三种组织谱系的研究进展发育成胎盘和胎膜，即滋养层细胞，胚外内胚层和胚外中胚层。四个项目研究几个超家族的单个膜的作用其产物与控制植入围植入期有关的基因发育和植入。其中包括细胞外基质。受体，或整合素、蛋白酶及其抑制物，多肽生长因子及其受体，以及促黄体生成素和其他转录因子。项目I研究了细胞外整合素受体的作用基质在滋养细胞和原始细胞围着床期发育中的作用项目II分析了螺旋-环-螺旋转录的作用调节人类滋养层细胞侵袭的因素；项目III研究细胞生长、分裂和分化的调控机制胚胎外谱系的形态发生及其在侵袭过程中的作用 Vivo；而Project VI分析了胚外中胚层，尤指尿囊膜。这些项目是由提供财政管理的行政核心提供支持，和两个研究核心，形态/细胞遗传学核心，它提供了组织学、原位杂交、共聚焦显微镜和染色体分析服务，以及细胞培养/靶向突变核心，其中提供细胞培养和基因转化服务，包括所有在小鼠身上进行定向突变所需的活性。建议数研究对人类健康问题有重大影响，包括不孕不育和早孕丢失综合征，低效通过医疗辅助产生的胚胎的植入率受孕，滋养细胞疾病，如绒毛膜癌，葡萄胎和先兆子痫。这些问题最终可能是只有通过哺乳动物研究才能解决，正如该计划所做的那样，它研究了胚胎和母体相互作用的基本机制。