EMBRYONIC STEM CELL AND EMBRYONIC GERM CELL LINE

胚胎干细胞和胚胎生殖细胞系

• 批准号: 7714972
• 负责人: Roger Arnold Pedersen
• 金额: $ 62.33万
• 依托单位: MAGEE-WOMEN'S RES INST AND FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7714972
• 关键词: Aliquot; Aneuploidy; Animal Model; Animals; Back; Cell Count; Cell Differentiation process; Cell Line; Cell surface; Cells; Chimera organism; Condition; Cryopreservation; Cultured Cells; DNA Fingerprinting; Development; EG Cell Line; Embryo; Ensure; Epigenetic Process; Extracellular Matrix; Fluorescent in Situ Hybridization; Freezing; Generations; Genetic; Germ Cells; Human; Human Development; Human Resources; In Vitro; Karyotype; Laboratories; Measures; Molecular; Monitor; Preparation; Primary Cell Cultures; Principal Investigator; Property; Quality Control; Research Personnel; Resources; Serum; Stem cells; Sterility; Techniques; Testing; Transplantation; Xenograft procedure; base; embryonic stem cell; human embryonic stem cell; improved; in vivo; nonhuman primate; pluripotency; quality assurance; scale up; stem; telomere

Each of the principal investigators for projects I-III is a recognized expert in human and non-human primate embryonic stem and germ cells. The Embryonic Stem Cell and Embryonic Germ Cell ("Stem and Germ Cell") Core draws on this combined expertise, thereby providing a central quality assurance and quality control laboratory for all cellular resources generated by the three projects. Three specific aims are proposed for this core in support of the three projects. Aim 1: Establish and maintain core resource of human and non-human primate embryonic stem cells for distribution to each of the projects and the other cores using good laboratory practice culture and frequent monitoring of pluripotency and aneuploidy. Aim 2: Establish and maintain core resource of non-human primate embryonic germ cells for distribution to each of the projects and the other cores using good laboratory practice culture and frequent monitoring of pluripotency and aneuploidy. Aim 3 (Stem Cell Genetic and Epigenetic Resource) Maintain sets of human and nonhuman primate ES and EG cell lines that are well-characterized with respect to their genetic and epigenetic status (whether normal or abnormal) for use in each of the projects and the other cores. The fulfillment of each of the three aims will support the three projects and the other cores in providing for in vitro molecular and cellular analysis, generation of chimeras (in the case of non-human primate ES and EG cells); and analysis of developmental fates, both in vitro and in vivo (the latter in transplantation studies involving animal models). By combining the expertise and resources of the three project leaders this core maximizes the available resources and reduces duplication and overlap.

项目I-III的每一位主要研究者都是人类和非人类灵长类动物胚胎发育方面公认的专家。 干细胞和生殖细胞。胚胎干细胞和胚胎生殖细胞（“干细胞和生殖细胞”）核心借鉴了这一点 结合专业知识，从而为所有蜂窝网络提供中央质量保证和质量控制实验室， 这三个项目产生的资源。为支持这三个项目，为这一核心提出了三个具体目标。 目的1：建立和维持人和非人灵长类胚胎干细胞的核心资源， 每个项目和其他核心使用良好实验室规范文化和多能性的频繁监测 和非整倍性。目的2：建立和维持非人灵长类胚胎生殖细胞的核心资源， 分发给每个项目和其他核心，使用良好实验室规范文化和经常监测 多能性和非整倍性。目的3（干细胞遗传和表观遗传资源）维持人类和非人类的干细胞集 灵长类ES和EG细胞系，其在遗传和表观遗传状态方面具有良好的特征 （不论正常或不正常），以供每一个项目和其他堆芯使用。三个方面的成就 aims将支持这三个项目和其他核心项目，提供体外分子和细胞分析， 嵌合体的产生（在非人灵长类动物ES和EG细胞的情况下）;以及发育命运的分析， 体外和体内（后者在涉及动物模型的移植研究中）。通过结合专业知识和 三个项目领导人的资源这个核心最大限度地利用现有资源，减少重复和重叠。