3 U24DK076126 -04 W1

3 U24DK076126 -04 W1

• 批准号: 7930219
• 负责人: RENEE C LEBOEUF
• 金额: $ 36.4万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-25 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7930219
• 关键词: Animal Model; Animals; Blood Vessels; Body Composition; Carbohydrates; Cardiovascular system; Complications of Diabetes Mellitus; Core Facility; Country; Diabetes Mellitus; Discipline; Disease; Energy Metabolism; Ensure; Evaluation; Eye; Goals; Health; Human Resources; Measures; Medical; Metabolic; Metabolic Diseases; Modeling; Molecular; Monitor; Morphology; Mus; Nature; Obesity; Operative Surgical Procedures; Pathology; Phenotype; Proteins; Renal function; Research; Research Activity; Research Personnel; Retinal Diseases; Technology; Universities; Washington; base; energy balance; heart function; human disease; interest; multidisciplinary; programs; response to injury; tissue preparation; type I and type II diabetes

DESCRIPTION, OVERALL (provided by applicant): The major goal of the Mouse Metabolic Phenotyping Center at the University of Washington is to promote and facilitate the study of animal models of obesity, diabetes and pathological complications due to these disorders. Because of the multidisciplinary nature of phenotyping approaches, close interaction across medical, pathological, and molecular disciplines is necessary to characterize animals used to study human diseases. By providing a number of Core facilities, the Seattle MMPC integrates and coordinates research activities so that Customers interested in having their animal models characterized can obtain a dear picture of the metabolic and disease state for each animal. The five Cores are: 1) an Administrative Core that is responsible for the day-to-day administration of the MMPC including the Pilot & Feasibility Program of the Animal Models of Diabetic Complications Consortium (AMDCC); 2) an Animal Core which will ensure proper delivery of animals and appropriate distribution to other Cores, and monitor health, husbandry and proper treatment of animals; 3) a Diabetes and Energy Balance Core to provide facilities for measuring body composition, energy expenditure, metabolic phenotyping and evaluation of type 1 and type 2 diabetes; 4) a Cardiovascular Core to provide assessment of heart function in normal and challenged states, vascular pathology quantifications, and response to injury modeling; 5) a Microvascular and Retinopathy Core which will evaluate kidney function and structural changes as well as extent of pathology in the eye. Four of the Core units are supported by HUBs which collect within each HUB, general technologies. Within the Animal Core is the Animal HUB which provides personnel to aid in dispersal of animals to the appropriate core facilities and to aid investigators in phenotyping. The Diabetes and Energy Balance Core harbors the Analytical HUB which is responsible for quantification of RNA, proteins, carbohydrates, and any other analyte needed in this MMPC. The Surgery HUB is within the Cardiovascular Core and provides surgery expertise for this Core and any surgery needs extending from other Cores. The Morphology HUB, located within the Microvascular/Retinopathy Core, is responsible for all tissue preparation and analysis. Thus, this MMPC provides outstanding facilities and support for the large and varied obesity and diabetes research base which includes our University, AMDCC investigators, and other potential Customers across the country.

总体描述（由申请人提供）： 华盛顿大学小鼠代谢表型中心的主要目标是促进和促进肥胖、糖尿病和这些疾病引起的病理并发症的动物模型的研究。由于表型分型方法的多学科性质，需要医学、病理学和分子学科之间的密切互动来表征用于研究人类疾病的动物。通过提供大量的核心设施，西雅图MMPC整合和协调研究活动，使客户有兴趣有他们的动物模型的特点，可以获得每只动物的代谢和疾病状态的清晰图片。这五个核心是：1）管理核心，负责MMPC的日常管理，包括糖尿病并发症动物模型联盟（AMDCC）的试点和可行性计划; 2）动物核心，确保动物的适当交付和适当分配给其他核心，并监测动物的健康、饲养和适当治疗; 3）糖尿病和能量平衡核心，以提供用于测量身体组成、能量消耗、代谢表型和评估1型和2型糖尿病的设施; 4）心血管核心，以提供在正常和挑战状态下的心脏功能的评估、血管病理学定量，以及对损伤建模的反应; 5）微血管和视网膜病变核心，其将评估肾功能和结构变化以及眼睛中的病理程度。核心单元中有四个由HUB提供支持，这些HUB在每个HUB内收集通用技术。动物中心内设有动物中心，提供人员协助将动物分散至适当的中心设施，并协助研究者进行表型分析。糖尿病和能量平衡核心包含分析HUB，负责定量该MMPC中所需的RNA、蛋白质、碳水化合物和任何其他分析物。手术中心位于心血管核心内，为该核心以及从其他核心延伸的任何手术需求提供手术专业知识。位于微血管/视网膜病变核心内的形态学HUB负责所有组织制备和分析。因此，该MMPC为大型和各种肥胖和糖尿病研究基地提供了出色的设施和支持，其中包括我们的大学，AMDCC研究人员和全国各地的其他潜在客户。