Core B and Tissue Core

核心B和组织核心

• 批准号: 7548839
• 负责人: RENEE C LEBOEUF
• 金额: $ 26.09万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7548839
• 关键词: Animal Experiments; Area; Arterial Fatty Streak; Atherosclerosis; Breeding; Cells; Collection; Diabetic mouse; Freezing; Immunofluorescence Microscopy; Immunohistochemistry; In Situ Hybridization; Individual; Longitudinal Studies; Maintenance; Methods; Modeling; Mus; Paraffin Embedding; Pathology; Process; Program Effectiveness; Research Personnel; Staining method; Stains; Technical Expertise; Techniques; Tissue Banks; Tissues; base; cost effectiveness; design; programs; sample fixation

The Mouse and Tissue Core (Core B) is designed to enhance the scientific objectives and cost-effectiveness of the Program Project in the following ways: 1) by serving as a central facility for the breeding and maintenance of mouse models of diabetes and atherosclerosis utilized by program project investigators, 2) by participating in the planning and implementation of longitudinal studies of these murine models, 3) by serving as a central facility for the collection and processing (freezing and/or fixation and paraffin embedding) of tissues and cells from these murine models, 4) by providing investigators with expertise in pathology-based analytical techniques such as histological staining, immunohistochemistry, immunofluorescence microscopy and in situ hybridization, most of which will be performed in this Core unit, and 5) by quantifying and characterizing atherosclerotic lesions and providing morphological characterization of other tissues taken from murine models used by program project investigators. Centralizing and standardizing planning, breeding and maintenance of mice for animal experiments, as well as tissue collection and methods of analyses in a single, integrated Core will increase the likelihood of obtaining reliable results and not compel individual Projects to perform duplicative animal experiments or to develop technical expertise in these areas. Further, carrying out these studies in a single, integrated Core will allow the results to be made available to each project in a way that facilitates Project interactions.

小鼠和组织核心（核心B）旨在增强科学目标和成本效益 通过以下方式实现该计划项目：1）作为育种的中心设施， 维持项目研究人员使用的糖尿病和动脉粥样硬化小鼠模型，2） 通过参与这些鼠模型的纵向研究的规划和实施，3）通过 用作收集和处理（冷冻和/或固定和石蜡）的中心设施 包埋）的组织和细胞从这些小鼠模型，4）通过提供研究人员的专业知识， 基于病理学的分析技术如组织学染色，免疫组织化学， 免疫荧光显微镜和原位杂交，其中大部分将在这个核心单位进行， 和5）通过量化和表征动脉粥样硬化病变并提供形态学表征 其他组织取自项目研究者使用的小鼠模型。集中和 规范动物实验用小鼠的计划、饲养和维护，以及组织 在一个单一的、综合的核心中收集和分析方法将增加获得 可靠的结果，不强迫个别项目进行重复的动物实验或开发 这些领域的技术专长。此外，在一个单一的综合核心中进行这些研究将使 以促进项目互动的方式向每个项目提供结果。