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Structural Basis of Polymerase Fidelity

Structural Basis of Polymerase Fidelity
聚合酶保真度的结构基础
批准号：
7734473
负责人：
JOHN W. DRAKE
金额：
$2.63万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/7734473
关键词：
Active SitesAmino AcidsAutomobile DrivingBacteriophage T4BacteriophagesBindingCongenital AbnormalityDNA biosynthesisDNA-Directed DNA PolymeraseDiseaseEnzymesExonucleaseFamilyGeneticGerm LinesHealthHumanIn VitroInduced MutationMaintenanceMalignant NeoplasmsMutationPlasmidsPolymeraseRateSomatic CellStructureSystemTestingWorkbacteriophage RB69 DNA polymerasebasein vivomitochondrial dysfunctionmutantreplicasespleen exonuclease

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中文摘要
翻译
RB69 DNA聚合酶结合口袋残基Tyr567先前被证明是保真度的关键决定因素：替换Y567A导致强大的突变子活性。Ser565是装订袋附近的一个组成部分。与Y567A不同，替换S565G对保真度的影响很小。然而，将Y567A与S565G结合，可以逆转Y567A的大部分突变子活性。这在聚合酶保真度的结构研究中是一个独特而有指导意义的观察，我们正在对这些结构在体内和体外的突变倾向进行详细的检查。
英文摘要
The RB69 DNA polymerase binding-pocket residue Tyr567 was shown previously to be a key determinant of fidelity: the replacement Y567A causes a powerful mutator activity. Ser565 is a nearby component of the binding pocket. Unlike Y567A, the replacement S565G has only a small impact on fidelity. However, combining Y567A and S565G reverses most of the mutator activity of Y567A. This is a unique and instructive observation in structural studies of polymerase fidelity, and we are working through a detailed examination of the mutational propensities of these constructs both in vivo and in vitro.
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Structural Basis Of Polymerase Fidelity
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