课题基金 / 基金详情

Structural Basis of Polymerase Fidelity

Structural Basis of Polymerase Fidelity
聚合酶保真度的结构基础
批准号：
7734473
负责人：
JOHN W. DRAKE
金额：
$2.63万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/7734473
关键词：
Active SitesAmino AcidsAutomobile DrivingBacteriophage T4BacteriophagesBindingCongenital AbnormalityDNA biosynthesisDNA-Directed DNA PolymeraseDiseaseEnzymesExonucleaseFamilyGeneticGerm LinesHealthHumanIn VitroInduced MutationMaintenanceMalignant NeoplasmsMutationPlasmidsPolymeraseRateSomatic CellStructureSystemTestingWorkbacteriophage RB69 DNA polymerasebasein vivomitochondrial dysfunctionmutantreplicasespleen exonuclease

项目摘要

项目成果

JOHN W. DRAKE的其他基金

相似基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
The RB69 DNA polymerase binding-pocket residue Tyr567 was shown previously to be a key determinant of fidelity: the replacement Y567A causes a powerful mutator activity. Ser565 is a nearby component of the binding pocket. Unlike Y567A, the replacement S565G has only a small impact on fidelity. However, combining Y567A and S565G reverses most of the mutator activity of Y567A. This is a unique and instructive observation in structural studies of polymerase fidelity, and we are working through a detailed examination of the mutational propensities of these constructs both in vivo and in vitro.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Direct Isolation of Chromosomal Regions for the Study of Human Genes
Structural Basis of Polymerase Fidelity
Replication Repair
Structural Basis Of Polymerase Fidelity
海外基金