The Novel Approach of RNA Based Therapeutic Technologies

基于 RNA 的治疗技术的新方法

• 批准号: 7762800
• 负责人: BA-BIE TENG
• 金额: $ 37.13万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7762800
• 关键词: Adenovirus Vector; Aftercare; Animals; Anti-Inflammatory Agents; Anti-inflammatory; Antiatherogenic; Aorta; Apolipoproteins B; Applications Grants; Arterial Fatty Streak; Arteries; Atherosclerosis; Blood Vessels; Cardiac; Cardiovascular system; Catalytic Domain; Catalytic RNA; Cells; Cessation of life; Cholesterol; Chylomicrons; Cleaved cell; Clinical; Collaborations; Collection; Complement; Consensus Sequence; Coronary; Coronary Arteriosclerosis; Critiques; Data; Defect; Development; Diet; Disease; Elements; Etiology; Event; Familial Hypercholesterolemia; Gene Delivery; Gene Expression; Genes; Genetic; Goals; Greater sac of peritoneum; Health; Heart Diseases; Human; Hyperlipidemia; Hypobetalipoproteinemia; Image; Inflammatory; Inflammatory Response; Ischemic Stroke; LDL Cholesterol Lipoproteins; Laboratories; Lead; Length; Life; Lipoproteins; Liver; Long-Term Effects; Low Density Lipoprotein Receptor; Low Density Lipoprotein oxidation; Low-Density Lipoproteins; Measures; Messenger RNA; Methods; Modality; Modeling; Modification; Molecular; Molecular Conformation; Molecular Weight; Morbidity - disease rate; Morphology; Mus; Nucleotides; Operative Surgical Procedures; Organ; Outcome Study; Oxidative Stress; Pathway interactions; Patients; Peritoneal Macrophages; Pharmacotherapy; Plasma; Prevention; Principal Investigator; Production; Proteins; RB1 gene; RNA; Risk; Role; Safety; Site; Small Intestines; Societies; Sodium Dodecyl Sulfate-PAGE; Staging; Structure; System; Technology; Testing; Therapeutic; Therapeutic Agents; Time; Tissues; Toxic effect; Transfection; Transgenic Mice; Viral Vector; Visual; adeno-associated viral vector; apolipoprotein B-48; atherogenesis; base; cardiovascular risk factor; cerebral artery; dalton; design; experience; gene therapy; gutted adenoviral vector; hammerhead ribozyme; in vitro Assay; in vivo; inflammatory marker; innovation; insight; interest; lipoprotein-associated phospholipase A(2); low density lipoprotein inhibitor; low density lipoprotein triglyceride; mRNA Expression; macrophage; mortality; mouse model; novel; novel strategies; novel therapeutics; particle; patient population; premature atherosclerosis; prevent; programs; research study; small molecule; stem; vector

DESCRIPTION (provided by applicant): The long-term objectives of this proposal are to design and develop novel strategies for regulating the development and progression of atherosclerosis and to provide new insights into the application of target specific hammerhead ribozymes for inhibiting gene expression. Our ultimate goal will be to develop human gene therapy strategies for alleviating atherosclerosis, which is responsible for most clinical manifestations of coronary artery disease and ischemic stroke. Atherosclerosis is recognized as an inflammatory disease, with the presence of LDL in the intima (subendothelial matrix) leading to inflammatory responses, oxidative stress, and plaque formation. Our laboratory has developed a genetically defined mouse model of atherosclerosis (LDb mice), which has a plasma lipoprotein profile that resembles humans with hyperlipidemia and results in diet-independent atherosclerosis. The etiology of atherosclerosis in this model mimics that of humans. In this application, we plan to develop novel strategies using minimal and tertiary stabilized hammerhead ribozymes to target two genes (apolipoprotein B mRNA and lipoprotein-associated phospholipase A2) simultaneously and will test the effect of this strategy on atherosclerosis development in LDb mice. We hypothesize that inhibiting gene expression of apoB and Lp-PLA2 mRNA will decrease oxidation of LDL, reduce the inflammatory response, and inhibit atherosclerosis development in LDb mice. We will use the double-stranded chimeric adeno-associated viral vector AAV2/8 to deliver these hammerhead ribozyme molecules to mice and will examine the persistence of both vectors and ribozyme gene expression in vivo. This study will allow us to understand the molecular mechanisms used by ribozymes to regulate gene expression and the ability of these ribozymes to inhibit atherosclerosis development. In summary, this study will provide a novel therapeutic treatment for coronary atherosclerosis and provide evidence of the efficiency and efficacy of RNA based molecules as potential therapeutic agents.

描述（由申请人提供）：本提案的长期目标是设计和开发用于调节动脉粥样硬化的发展和进展的新策略，并为靶特异性锤头状核酶用于抑制基因表达的应用提供新的见解。我们的最终目标将是开发人类基因治疗策略，以减轻动脉粥样硬化，这是负责冠状动脉疾病和缺血性中风的大多数临床表现。动脉粥样硬化被认为是一种炎症性疾病，内膜（内皮下基质）中LDL的存在导致炎症反应、氧化应激和斑块形成。我们的实验室已经开发了一种遗传定义的动脉粥样硬化小鼠模型（LDb小鼠），其血浆脂蛋白谱类似于患有高脂血症的人类，并导致非饮食依赖性动脉粥样硬化。该模型中动脉粥样硬化的病因学模拟了人类的病因学。在本申请中，我们计划开发新的策略，使用最小和三级稳定锤头状核酶，同时靶向两个基因（载脂蛋白B mRNA和脂蛋白相关磷脂酶A2），并将测试这种策略对LD B小鼠动脉粥样硬化发展的影响。我们推测，抑制apoB和Lp-PLA 2 mRNA的基因表达将减少LDL的氧化，减少炎症反应，并抑制LDb小鼠动脉粥样硬化的发展。我们将使用双链嵌合腺相关病毒载体AAV 2/8将这些锤头状核酶分子传递给小鼠，并将检查载体和核酶基因在体内表达的持久性。这项研究将使我们了解核酶用于调节基因表达的分子机制，以及这些核酶抑制动脉粥样硬化发展的能力。 总之，这项研究将为冠状动脉粥样硬化提供一种新的治疗方法，并提供基于RNA的分子作为潜在治疗剂的效率和功效的证据。