Core Rederivation and Barrier Renovation

描述（由申请人提供）：肯塔基州大学（英国）要求6,432,134美元，以翻新5,526平方米。英尺在桑德斯-布朗老年中心的四楼拟议的翻新工程将彻底拆除和拆除现有的陈旧和过时的小型屏障动物设施。新设施将提供三个急需的具体核心能力，目前还没有在英国。将建造和配备一个中央核心胚胎冷冻保存和胚胎移植实验室。该实验室将提供内部储备和进口转基因菌株的胚胎移植再衍生，以消除可能改变研究结果的致病或寄生生物。此外，该实验室将为英国研究人员提供精子和胚胎冷冻保存服务，允许长期储存独特的转基因品系，作为连续育种的替代方案，或在发生意外菌落损失时作为研究资源。该实验室将毗邻一个小型的高安全性无菌屏障，用于在转移到传统动物设施之前对再衍生菌株进行初始扩增。最后，将建造一个供无菌啮齿动物居住的房间，并配备三个柔性薄膜隔离器，以支持对具有明确微生物植物群的转基因菌株的迅速增长的需求。通过将这三个设施集中在同一地点，我们将最大限度地利用现有的支持灭菌设备，提供一系列核心服务，这些服务已被调查人员确定为关键基础设施需求。

DESCRIPTION (provided by applicant): The University of Kentucky (UK) requests $6,432,134 to renovate 5,526 sq. ft. on the fourth floor of the Sanders-Brown Center for Aging. The proposed renovation will completely demolish and remove the present aged and obsolete small barrier animal facility. The new facility will provide three critically needed specific core capabilities that are presently not available at UK. A centralized core embryo cryopreservation and embryo transfer laboratory will be constructed and outfitted. This laboratory will provide embryo transfer rederivation of both in-house stock and imported transgenic strains to eliminate pathogenic or commensal organisms potentially altering research results. Additionally this laboratory will provide both sperm and embryo cryopreservation services to UK investigators permitting the long- term storage of unique transgenic strains as an alternative to continual breeding or as a research resource in the event of unanticipated colony loss. This laboratory will be adjacent to a small high- security sterile barrier for initial expansion of rederived strains prior to movement to conventional animal facilities. Finally, a room for gnotobiotic rodent housing will be constructed and outfitted with three flexible film isolators to support the burgeoning need for transgenic strains with defined microbial flora. By locating these three facilities in this single location we will maximally leverage the available support sterilization equipment to provide a set of core services that have been identified by the investigators as a critical infrastructure need.