CORE--ANIMAL BARRIER FACILITY

核心——动物屏障设施

• 批准号: 6268778
• 负责人: JEFFREY A BLUESTONE
• 金额: $ 10.11万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6268778
• 关键词: animal colony; biomedical facility; germ free condition; laboratory mouse

The goal of the University of Chicago Cancer Research Center (UCCRC) Animal Barrier Facilities is to provide Cancer Center members the ability to conduct studies with rodents free of intercurrent infection with adventitious murine pathogens. The Barriers are supported by the facilities, programs, and staff of the University's Animal Resources Center (ARC). The ARC's Comparative Diagnostic Laboratory(CDL) provides expertise in rodent serology, microbiology, parasitology, and hematology. All biological materials introduced into rodents maintained in these facilities are evaluated by the CDL for contamination with adventitious viruses and mycoplasma by MAP testing. The ARC conducts an extensive Murine Health Monitoring Program to confirm that the pathogen-free status of the barrier colonies are maintained. The program is designed to detect variance in health status at specific time intervals. The program employs sentinel animals monitored monthly, serum banking, evaluation of "index" cases and close communication with investigative staff who may note unexplained variances in their experiments. Additionally, a quarantine program which includes extensive diagnostic evaluation is instituted for all rodents imported from colonies obtained from non-commercial vendors. Quarantine is essential to minimize the likelihood of introducing unwanted murine pathogens into barrier colonies. The rodent barrier facilities serving the UCCRC are located on the top floor of the Franklin McLean Institute (FMI) and in the basement of Biological Sciences Learning Center (BSLC). They are essential components of the Cancer Research Center, providing approximately 7,925 net square feet of animal holding and procedure space. The facilities contain sixteen animal holding rooms, seven of which are equipped with three Illinois cubicles. In addition to animal holding rooms, the barriers are equipped with laboratory and procedure space. Isolator type caging systems are employed throughout the barriers. All caging materials and feed are autoclaved prior to use. Animals are transferred within a separate hood or within a cubicle by animal care specialists, using chemically disinfected forceps between cage transfers. A maximum of four or five mice, dependent on weight, are permitted per cage. A bar coded cage card provides encrypted information including the strain or stock, source, and investigator. Cage cards are routinely scanned for information which is downloaded into the ARC database that is used to maintain accurate and detailed census information.

芝加哥大学癌症研究中心（UCCRC）的目标是 动物屏障设施是为癌症中心成员提供能力， 对没有并发感染的啮齿动物进行研究， 外源性鼠病原体。这些障碍得到了 大学动物资源部的设施、项目和工作人员 中心（ARC）。ARC的比较诊断实验室（CDL）提供 啮齿动物血清学、微生物学、寄生虫学和血液学方面的专业知识。 所有引入啮齿动物体内的生物材料都保存在这些 由CDL评估设施的外源性污染 通过MAP检测病毒和支原体。ARC进行了广泛的 小鼠健康监测计划，以确认无病原体状态 屏障菌落的数量得以维持。该程序旨在检测 在特定时间间隔内的健康状况差异。该方案采用 每月监测哨兵动物，血清库，“指数”评价 调查人员可能注意到， 无法解释的实验差异此外，隔离 一个包括广泛诊断评估的计划被制定， 从非商业供应商处获得的种群进口的所有啮齿动物。 隔离对于最大限度地减少 不需要的鼠病原体进入屏障菌落。 该中心的防鼠屏障设施位于顶层 富兰克林姆克林研究所（FMI）的地板和地下室 生物科学学习中心（BSLC）。它们是必不可少的组件 癌症研究中心，提供约7，925净平方米 动物饲养英尺和手术空间。设施包括 十六个动物收容室，其中七个配备了三个 伊利诺斯州橄榄球队。除了动物收容室外， 配备实验室和手术空间。隔离器式屏蔽 系统在整个屏障中使用。所有笼形材料和 饲料在使用前进行高压灭菌。动物在一个 由动物护理专家使用单独的罩子或在小隔间内 化学消毒的镊子。最多四 或每笼允许5只小鼠（取决于体重）。条形码可 笼卡提供包括菌株或原种的加密信息， 消息来源和调查员。定期扫描笼卡， 下载到ARC数据库的信息，用于 保持准确和详细的普查信息。