Boston OAIC: A Translational Approach to Function Promoting Therapies

波士顿 OAIC：功能促进疗法的转化方法

• 批准号: 10752906
• 负责人: SHALENDER BHASIN
• 金额: $ 39.37万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10752906
• 关键词: 19 year old; Administrative Supplement; Adult; Aging; American; Area; Behavior Therapy; Biological; Biological Assay; Biological Markers; Biometry; Blood; Boston; Calibration; Capsicum; Cell physiology; Centers for Disease Control and Prevention (U.S.); Chronic Disease; Clinical Trials; Communities; Connecticut; Data Science Core; Development; Disease; Elderly; Ensure; Exposure to; Fostering; Funding; GDF8 gene; Gender; General Hospitals; Generations; Geroscience; Growth Factor; Health; Hormones; Immunoassay; Interdisciplinary Study; Investigation; Laboratories; Leadership; Link; Liquid Chromatography; Measurement; Mentors; Mus; National Institute on Aging; Nicotinamide adenine dinucleotide; Nutritional; Oregon; Organism; Outcome; Oxytocin; Parabiosis; Peptides; Phenotype; Population Heterogeneity; Positioning Attribute; Procedures; Proteins; Reporting; Research; Research Personnel; Resources; Risk Factors; Science; Signal Pathway; Source; Standardization; Talents; Technology; Therapeutic Intervention; Training; Translational Research; United States National Institutes of Health; Universities; Validation; Variant; age related; age stratification; burden of illness; education research; epidemiology study; ethnic diversity; experimental study; ghrelin; morphogens; novel therapeutics; parent grant; parent project; pharmacologic; pre-clinical; prevent; programs; racial diversity; randomized trial; recruit; sex; tandem mass spectrometry; therapeutic candidate; translational approach; translational pipeline

Summary The overall objective of this administrative supplement is to support a developmental project linked to the Translational Discovery Core of the Boston Older Americans Independence Center. The primary aim of the project is to optimize liquid chromatography tandem mass spectrometry (LC-MS/MS) assays for the measurement of biologically active mature forms of GDF11, myostatin, oxytocin, and acyl ghrelin that do not have any interference from respective proproteins or related peptides, and that are calibrated against well- characterized standards generated by the CDC's Hormone Standardization Program. The CDC laboratory will generate high quality reference materials to establish the traceability of procedure to a value that represents the best estimate of the true value. The generation of a commutable reference material by the CDC Laboratory will ensure accuracy, and help reduce inter-assay and inter-laboratory variability. Upon successful completion of this developmental project, these harmonized assays will be made available to the investigators within the Boston OAIC and other OAICs, as well as to the scientific community at large through the Translational Discovery Core of the Boston OAIC. These assays will be of substantial value to NIA- funded investigators within Boston OAIC (Bhasin, Wagers, Lee), other OAICs (Lee at University of Connecticut OAIC, Farmington, CT; investigators associated with the CARGO Project) and investigators outside the Pepper Center Network (Lawson at Mass General Hospital; Orwoll at the University of Oregon, Cawthon at UCSF). The availability of accurate and sensitive assays would be a useful resource to many researchers and would extend the range and quality of this NIH-funded research. The project represents a historical opportunity and an important step towards harmonization of optimized assays for the measurement of geronic factors and their related proteins and peptides.

总结 这份行政补充文件的总体目标是支持一个与 波士顿老年美国人独立中心（Boston Older Americans Independence Center）的主要目的 该项目是优化液相色谱串联质谱（LC-MS/MS）分析， 测量GDF 11、肌肉生长抑制素、催产素和酰基生长素释放肽的生物活性成熟形式， 具有来自相应前蛋白或相关肽的任何干扰，并且针对良好的 由CDC的激素标准化计划产生的特征标准。CDC实验室将 生成高质量的参考材料，以建立程序的可追溯性， 真实价值的最佳估计。CDC实验室生成可交换的参考物质 将确保准确性，并有助于减少试验间和实验室间的变异性。 在成功完成该开发项目后，这些协调的检测试剂盒将提供给 波士顿OAIC和其他OAIC的调查人员以及整个科学界 通过波士顿OAIC的翻译发现核心。这些分析将对NIA具有重大价值- 波士顿OAIC（Bhasin，Wagers，Lee），其他OAIC（康涅狄格大学的Lee） OAIC，法明顿，CT;与CARGO项目相关的调查人员）和Pepper以外的调查人员 中心网络（马萨诸塞州总医院的劳森;俄勒冈州大学的奥沃尔，加州大学旧金山分校的考顿）。 准确和灵敏的测定的可用性将是许多研究人员的有用资源， 扩大NIH资助研究的范围和质量。该项目代表了一个历史机遇， 一个重要的步骤，协调优化测定老年因素及其 相关蛋白质和肽。