Boston OAIC: A Translational Approach to Function Promoting Therapies

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Summary The overall objective of this administrative supplement is to support a developmental project linked to the Translational Discovery Core of the Boston Older Americans Independence Center. The primary aim of the project is to optimize liquid chromatography tandem mass spectrometry (LC-MS/MS) assays for the measurement of biologically active mature forms of GDF11, myostatin, oxytocin, and acyl ghrelin that do not have any interference from respective proproteins or related peptides, and that are calibrated against well- characterized standards generated by the CDC's Hormone Standardization Program. The CDC laboratory will generate high quality reference materials to establish the traceability of procedure to a value that represents the best estimate of the true value. The generation of a commutable reference material by the CDC Laboratory will ensure accuracy, and help reduce inter-assay and inter-laboratory variability. Upon successful completion of this developmental project, these harmonized assays will be made available to the investigators within the Boston OAIC and other OAICs, as well as to the scientific community at large through the Translational Discovery Core of the Boston OAIC. These assays will be of substantial value to NIA- funded investigators within Boston OAIC (Bhasin, Wagers, Lee), other OAICs (Lee at University of Connecticut OAIC, Farmington, CT; investigators associated with the CARGO Project) and investigators outside the Pepper Center Network (Lawson at Mass General Hospital; Orwoll at the University of Oregon, Cawthon at UCSF). The availability of accurate and sensitive assays would be a useful resource to many researchers and would extend the range and quality of this NIH-funded research. The project represents a historical opportunity and an important step towards harmonization of optimized assays for the measurement of geronic factors and their related proteins and peptides.